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Glucosinolate Profiles Change During the Life Cycle and Mycorrhizal Colonization in a Cd/Zn Hyperaccumulator Thlaspi praecox (Brassicaceae)

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Abstract

Thlaspi praecox Wulfen (Brassicaceae) is a perennial Cd/Zn hyperaccumulating plant species that forms functional arbuscular mycorrhizal (AM) symbiosis. Glucosinolates (GS) were studied in different organs of field-collected T. praecox at differing plant developmental stages. Additionally, AM colonization was recorded. Total GS concentrations and profiles of nine individual GS varied during the plant life cycle. Novel individual GS that were related to specific developmental phases, mainly to flowering and seed production, were identified. The highest total GS and sinalbin concentrations in rosette leaves were found in the vegetative phase, possibly contributing to protection of young, palatable leaves. The lowest were found in roots during the flowering and the seeding phases. Increased total GS concentrations in roots and enhanced aliphatic GS, especially glucobrassicanapin, in the senescence phase may protect roots from herbivory during winter and early spring. The presence of glucotropaeolin and the absence of glucobrassicanapin in the flowering phase coincided with peak AM colonization. This is the first report on GS profiles in an AM and metal-hyperaccumulating plant.

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Abbreviations

GS:

Glucosinolates

AM:

Arbuscular mycorrhiza

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Acknowledgments

The work was supported by the following projects: MSZS P1-0212 Biology of Plants research program, “Young researchers”, EU COST 859, Spanish and Catalonian Governments (DGICYT, BFU 2004-02237-CO2-01, Group de Recerca 2005GR 0078) and Spanish Ministry of Education and Science (BFU 2007-60332/BFI). The grant by COST Action 859 to P. Pongrac for her research stay at the Autonomous University of Barcelona is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, 1000, Ljubljana, Slovenia

    Paula Pongrac, Katarina Vogel-Mikuš & Marjana Regvar

  2. Laboratorio de Fisiología Vegetal, Facultad de Ciencias, Universidad Autónoma de Barcelona, 08193, Bellaterra, Spain

    Roser Tolrà, Charlotte Poschenrieder & Juan Barceló

Authors
  1. Paula Pongrac
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  2. Katarina Vogel-Mikuš
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  3. Marjana Regvar
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  4. Roser Tolrà
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  5. Charlotte Poschenrieder
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  6. Juan Barceló
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Correspondence to Marjana Regvar.

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Pongrac, P., Vogel-Mikuš, K., Regvar, M. et al. Glucosinolate Profiles Change During the Life Cycle and Mycorrhizal Colonization in a Cd/Zn Hyperaccumulator Thlaspi praecox (Brassicaceae). J Chem Ecol 34, 1038–1044 (2008). https://doi.org/10.1007/s10886-008-9502-7

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  • DOI: https://doi.org/10.1007/s10886-008-9502-7

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