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Cutaneous mitochondrial respirometry: non-invasive monitoring of mitochondrial function

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Abstract

The recently developed technique for measuring cutaneous mitochondrial oxygen tension (mitoPO2) by means of the Protoporphyrin IX—Triplet State Lifetime Technique (PpIX-TSLT) provides new opportunities for assessing mitochondrial function in vivo. The aims of this work were to study whether cutaneous mitochondrial measurements reflect mitochondrial status in other parts of the body and to demonstrate the feasibility of the technique for potential clinical use. The first part of this paper demonstrates a correlation between alterations in mitochondrial parameters in skin and other tissues during endotoxemia. Experiments were performed in rats in which mitochondrial dysfunction was induced by a lipopolysaccharide-induced sepsis (n = 5) and a time control group (n = 5). MitoPO2 and mitochondrial oxygen consumption (mitoVO2) were measured using PpIX-TSLT in skin, liver and buccal mucosa of the mouth. Both skin and buccal mucosa show a significant mitoPO2-independent decrease (P < 0.05) in mitoVO2 after LPS infusion (a decrease of 37 and 39 % respectively). In liver both mitoPO2 and mitoVO2 decreased significantly (33 and 27 % respectively). The second part of this paper describes the clinical concept of monitoring cutaneous mitochondrial respiration in man. A first prototype of a clinical PpIX-TSLT monitor is described and its usability is demonstrated on human skin. We expect that clinical implementation of this device will greatly contribute to our understanding of mitochondrial oxygenation and oxygen metabolism in perioperative medicine and in critical illness. Our ultimate goal is to develop a clinical monitor for mitochondrial function and the current results are an important step forward.

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Acknowledgments

This work was financially supported by a Life Sciences Pre-Seed Grant (Grant No. 40-41300-98-9037) from the Netherlands Organization for Health Research and Development (ZonMW).

Conflict of interest

Dr. E.G. Mik is founder and shareholder of Photonics Healthcare B.V., a company aimed at making the delayed fluorescence lifetime technology clinically available. Photonics Healthcare B.V. holds the exclusive licenses to several patents regarding this technology, filed and owned by the Academic Medical Center in Amsterdam and the Erasmus Medical Center in Rotterdam, The Netherlands.

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Authors and Affiliations

  1. Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC – University Medical Center Rotterdam, ‘s-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands

    Floor A. Harms, Sander I. A. Bodmer, Nicolaas J. H. Raat & Egbert G. Mik

  2. Department of Intensive Care, Erasmus MC – University Medical Center Rotterdam, ‘s-Gravendijkwal 230, 3015 CE, Rotterdam, The Netherlands

    Egbert G. Mik

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  1. Floor A. Harms
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  2. Sander I. A. Bodmer
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  3. Nicolaas J. H. Raat
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  4. Egbert G. Mik
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Correspondence to Floor A. Harms.

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Harms, F.A., Bodmer, S.I.A., Raat, N.J.H. et al. Cutaneous mitochondrial respirometry: non-invasive monitoring of mitochondrial function. J Clin Monit Comput 29, 509–519 (2015). https://doi.org/10.1007/s10877-014-9628-9

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  • DOI: https://doi.org/10.1007/s10877-014-9628-9

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