Abstract
In this study, silver nanoparticle was green synthesized using the leaf extract of Solanum nigrum (Sn-AgNPs) and bio-physically characterized by UV–Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), High resolution transmission electron microscopy (HR-TEM), Zeta potential analysis and Energy dispersive X-ray (EDX) analysis. The ecotoxicity of silver nanoparticle (Sn-AgNPs) were tested against both invertebrate (Ceriodaphnia cornuta and Paramecium sp.) and vertebrate aquatic animal models (Guppy fish, Poecilia reticulata) in comparison with bare silver nitrate. Sn-AgNPs were observed to be less toxic than ionic silver (silver nitrate). The ecotoxicity levels of Sn-AgNPs were found to be varied between tested organisms. Sn-AgNPs caused 100% mortality of freshwater crustacean, C. cornuta at 50 µg mL−1. At concentration below 50 µg mL−1 (10–30 µg mL−1), abnormality in the swimming behavior of C. cornuta was noticed. The ingestion and accumulation of Sn-AgNPs in the intestine of C. cornuta neonates were visualized under light and confocal laser scanning microscopic images. The ecotoxicity of Sn-AgNPs to the freshwater protozoan ciliate, Paramecium sp. showed that 30 µg mL−1 were lethal and produced 100% mortality at the same concentration. The study concludes that Sn-AgNPs was less toxic to both invertebrate and vertebrate models compared to ionic silver nitrate.
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Acknowledgements
The authors thank the RUSA phase 2.0 grant [Ref-24-51-2014-U policy] TN Multi-Gen. Department of Education, Government of India. The corresponding author Dr. B. Vaseeharan thanks the Department of Biotechnology (DBT), New Delhi, India, for financial assistance under the Project Grants Code: BT/PR7903/AAQ/3/638/2013. The third author S. Vijayakumar (SRF) thanks the DST, New Delhi, India for financial support under INSPIRE programme (INSPIRE Fellow-IF140145). The authors gratefully acknowledge the University Scientific Instrumentation Centre (USIC) for providing Confocal laser scanning microscopy, XRD and FTIR instrumental facilities to this research.
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Jenifer, A.A., Malaikozhundan, B., Vijayakumar, S. et al. Green Synthesis and Characterization of Silver Nanoparticles (AgNPs) Using Leaf Extract of Solanum nigrum and Assessment of Toxicity in Vertebrate and Invertebrate Aquatic Animals. J Clust Sci 31, 989–1002 (2020). https://doi.org/10.1007/s10876-019-01704-7
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DOI: https://doi.org/10.1007/s10876-019-01704-7