Skip to main content
SpringerLink
Log in

Piper betle: Augmented Synthesis of Gold Nanoparticles and Its In-vitro Cytotoxicity Assessment on HeLa and HEK293 Cells

  • Original Paper
  • Published:
Journal of Cluster Science Aims and scope Submit manuscript

Abstract

Plant-based photo fabrication of noble metal nanoparticles is a dominating research area in nanobiotechnology as they offer immense applications in biomedicine, drug delivery and bioimaging. In this study, we report the synthesis of gold nanoparticles (GNPs) using Piper betle leaf extract. Extraction of secondary metabolites was performed by both acetonic and ethanolic extract to establish a comparative assessment. UV–Visible spectroscopic, Transmission Electron Microscopy, Energy Dispersive X-ray and X-ray diffraction characterization confirmed the synthesis of GNPs. The results thus suggested that the metabolites of P. betle L. leaf can effectively catalyze the conversion of Au3+ to Au0 ions and serve as excellent stabilizing agents. Morphological modulation was found with varying tetrachloroauric acid concentration and the solvent medium used. Cytotoxicity assessment of GNPs on HeLa and HEK293 signified the selective toxic behaviour of GNPs towards cancerous cell lines. Further, biocompatibility towards HEK293 cells signified that the GNPs can be used for drug delivery as well as bio-imaging applications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. P. Boomi, G. P. Poorani, S. Palanisamy, S. Selvam, G. Ramanathan, S. Ravikumar, H. Barabadi, H. G. Prabhu, J. Jeyakanthan, and M. Saravanan (2019). J. Clust. Sci.. https://doi.org/10.1007/s10876-019-01530-x).

    Article  Google Scholar 

  2. H. Barabadi, M. A. Mahijoub, B. Tajani, A. Ahmadi, Y. Junejo, and M. Saravanan (2019). J. Clust. Sci.30, 259. https://doi.org/10.1007/s10876-018-01491-7.

    Article  CAS  Google Scholar 

  3. D. Thompson (2007). Gold Bull.40, 4.

    Google Scholar 

  4. D. Mukundan, R. Mohankumar, and R. Vasanthakumari (2017). Bull. Mater. Sci.40, 2.

    Google Scholar 

  5. P. Suchomel, L. Kvitek, R. Prucek, A. Panacek, A. Halder, S. Vajda, and R. Zboril (2018). Sci. Rep.8, 4589. https://doi.org/10.1038/s41598-018-22976-5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. P. Logeswari, S. Silambarasan, and J. Abraham (2012). J. Saudi Chem. Soc.4, 23.

    Google Scholar 

  7. G. F. Paciotti, D. G. I. Kingstone, and L. Tamarkin (2006). Drug Dev. Res.67, 47.

    CAS  Google Scholar 

  8. P. Mohanpuria, N. K. Rana, and S. K. Yadav (2008). J. Nanopart. Res.10, 507.

    CAS  Google Scholar 

  9. V. Kumar and S. K. Yadav (2009). J. Chem. Technol. Biotechnol.84, 151.

    CAS  Google Scholar 

  10. J. Y. Song and B. S. Kim (2009). Korean J. Chem. Eng.25, 808.

    Google Scholar 

  11. S. Narayan, R. S. Devi, and C. S. S. Devi (2007). Chem. Biol. Interact.170, 67.

    CAS  PubMed  Google Scholar 

  12. R. Sathyavathi, M. B. Krishna, S. V. Rao, R. Saritha, and D. N. Rao (2010). Adv. Sci. Lett.3, 6.

    Google Scholar 

  13. M. Keshavamurthy, B. S. Srinath, and R. Ravishankar (2018). Part. Sci. Technol.36, 7.

    Google Scholar 

  14. B. Patra, S. N. Pradhan, M. T. Das, and S. K. Dey (2018). Int. J. Recent Sci. Res.9, 4.

    Google Scholar 

  15. B. Patra and S. N. Pradhan (2018). J. Exp. Sci.9, 4.

    Google Scholar 

  16. R. S. Patil, P. M. Harale, K. V. Shivangekar, P. P. Kumbhar, and R. R. Desai (2015). J. Chem. Pharm. Res.7, 5.

    Google Scholar 

  17. H. Katas, N. Z. Moden, C. S. Lim, T. Celesistinus, J. Y. Chan, P. Ganasan, and S. S. I. Abdalla (2018). J. Nanotechnol.. https://doi.org/10.1155/2018/4290705.

    Article  Google Scholar 

  18. R. Karthik, S. Mingchen, A. Elangovan, P. Muthukrishnan, R. Shanmugam, and B. Showlou (2016). J.Colloid Interface Sci.468, 163.

    CAS  PubMed  Google Scholar 

  19. A. Jafarizad, K. Safaee, S. Gharibian, Y. Omidi, and D. Ekinci (2015). Procedia Mater. Sci.11, 224.

    CAS  Google Scholar 

  20. C. Jayaseelan, R. Ramkumar, A. A. Rahman, and P. Perumal (2013). Ind. Crop. Prod.45, 423.

    CAS  Google Scholar 

  21. R. Majumdar, B. G. Bag, and N. Maity (2013). Int. Nano Lett.3, 53.

    Google Scholar 

  22. J. K. Andeani, H. Kazemi, S. Mohsenzadeh, and A. Safavi (2011). Dig. J. Nanomater. Biostruct.6, 1011.

    Google Scholar 

  23. R. Kirubha and G. Alagumuthu (2014). Int. J. Pharm.4, 195.

    Google Scholar 

  24. S. P. Chandran, M. Chaudhary, R. Pasricha, and M. A. Ahmed (2006). Biotechnol. Prog.22, 577.

    CAS  PubMed  Google Scholar 

  25. D. S. Sheny, J. Mathew, and D. Philip (2011). Spectrochim. Acta A79, 254.

    CAS  Google Scholar 

  26. N. Basavegowda, A. Sobczak-Kupiec, D. Malina, H. S. Yathirajan, V. R. Keerthi, and N. Chandrashekar (2013). Adv. Mater. Lett.4, 332.

    CAS  Google Scholar 

  27. N. Basavegowda, G. D. Kumar, B. Tyliszczak, Z. Wzorek, and A. Sobczak-Kupiec (2015). Ann. Agric. Environ. Med.22, 84.

    CAS  PubMed  Google Scholar 

  28. R. Radha, M. Murugalakshmi, and S. Kokila Rani (2016). Int. J. Interdiscip. Res.2, 306.

    Google Scholar 

  29. M. N. Nadagouda, N. Iyanna, J. Lalley, C. Han, D. D. Dionysiou, and R. S. Varma (2014). ACS Sustain. Chem. Eng.2, 1717.

    CAS  Google Scholar 

  30. P. Kumar, P. Singh, K. Kumari, S. Mozumdar, and R. Chandra (2011). Mater. Lett.65, 595.

    CAS  Google Scholar 

  31. A. R. Vilchis-Nestor, V. Sánchez-Mendieta, R. M. Gómez-Espinosa, M. Camacho-López, and J. A. Arenas-Alatorre (2008). Mater. Lett.62, 3103.

    CAS  Google Scholar 

  32. R. K. Das, B. B. Borthakur, and U. Bora (2010). Mater. Lett.64, 1445.

    CAS  Google Scholar 

  33. A. D. Dwivedi and K. Gopal (2010). Colloids Surf. A369, 27.

    CAS  Google Scholar 

  34. Q. Liu, H. Liu, Z. Yuan, D. Wei, and Y. Ye (2012). Colloids Surf. B92, 348.

    CAS  Google Scholar 

  35. S. L. Smitha, D. Philip, and K. G. Gopchandran (2009). Spectrochim. Acta A74, 735.

    CAS  Google Scholar 

  36. M. V. Sujitha and S. Kannan (2013). Spectrochim. Acta A102, 15.

    CAS  Google Scholar 

  37. N. K. Reddy Bogireddy, L. Martinez Gomez, I. Osorio-Roman, and V. Agarval (2017). Adv. Nano Res.5, 253.

    Google Scholar 

  38. K. B. Narayanan and N. Sakthivel (2010). Mater. Charact.61, 1232.

    CAS  Google Scholar 

  39. K. B. Narayanan and N. Sakthivel (2008). Mater. Lett.62, 4588.

    CAS  Google Scholar 

  40. L. Olenic and I. Chiorean (2015). Int. J. Latest Res. Sci. Technol.4, 16.

    Google Scholar 

  41. G. Sathishkumar, K. J. Pradeep, V. Vignesh, C. Rajkuberan, M. Jeyaraj, and M. Selvakumar (2016). J. Mol. Liq.215, 229.

    Google Scholar 

  42. R. Vijayakumar, V. Devi, K. Adavallan, and D. Saranya (2011). Phys. E44, 665.

    CAS  Google Scholar 

  43. T. I. Shalaby, R. S. Shams El-Dine, and S. A. Abd El-Gaber (2015). Nanosci. Nanotechnol.5, 89.

    CAS  Google Scholar 

  44. K. Sneha, M. Sathish Kumar, S. Y. Lee, M. A. Bae, and Y. S. Yun (2011). J. Nanosci. Nanotechnol.11, 1811.

    CAS  PubMed  Google Scholar 

  45. S. S. Shankar, A. Rai, A. Ahmad, and M. Sastry (2005). Chem. Mater.17, 566.

    CAS  Google Scholar 

  46. S. Ghosh, S. Patil, M. Ahire, R. Kitture, A. Jabgunde, and S. Kale (2011). J. Nanomater. 45, 1. https://doi.org/10.1155/2011/354793.

    Article  CAS  Google Scholar 

  47. Y. Song, H. K. Jang, and B. S. Kim (2009). Process Biochem.44, 1133.

    CAS  Google Scholar 

  48. A. Annamalai, V. L. Christina, D. Sudha, M. Kalpana, and P. T. Lakshmi (2013). Colloids Surf. B108, 60.

    CAS  Google Scholar 

  49. A. Rajan, M. Meena Kumari, and D. Philip (2014). Spectrochim. Acta A118, 793.

    CAS  Google Scholar 

  50. K. X. Lee, K. Shameli, M. Miyake, N. Kuwano, N. B. Khairudin, and S. E. B. Mohamad (2016). J. Nanomater.1, 2.

    Google Scholar 

  51. B. Kumar, K. Smita, L. Cumbal, J. Camacho, Gallegos E. Hernández-, M. De Guadalupe Chávez-López, and M. Grijalva (2016). Mater. Sci. Eng. C62, 725.

    CAS  Google Scholar 

  52. S. Lokina and V. Narayanan (2013). Chem. Sci. Trans.2, 105.

    Google Scholar 

  53. J. Kasthuri, S. Veerapandian, and N. Rajendiran (2009). Colloids Surf. B68, 55.

    CAS  Google Scholar 

  54. G. Barman, S. Maiti, and J. Konar Laha (2013). Nanoscale Res. Lett.8, 181.

    PubMed  PubMed Central  Google Scholar 

  55. S. A. Aromal, V. K. Vidhu, and D. Philip (2012). Spectrochim. Acta A85, 99.

    CAS  Google Scholar 

  56. S. Lokina and V. Narayanan (2017). Int. J. Pharm. Bio. Sci.8, 203.

    CAS  Google Scholar 

  57. D. Philip (2010). Spectrochim. Acta A77, 807.

    Google Scholar 

  58. T. Muralikrishna, R. Malothu, M. Pattanayak, and P. L. Nayak (2014). World J. NanoSci. Technol.3, 66.

    Google Scholar 

  59. L. Gardea-Torresdey, J. G. Persons, E. Gomez, J. Peralta-Videa, H. E. Troiani, and P. Santiago (2002). Nano Lett.2, 397.

    CAS  Google Scholar 

  60. T. Elavazhagan and K. D. Arunachalam (2011). Int. J. Nanomed.6, 1265.

    CAS  Google Scholar 

  61. D. M. Ali, N. Thajuddin, K. Jeganathan, and M. Gunasekaran (2011). Colloids Surf. B85, 360.

    Google Scholar 

  62. P. S. Vankar and D. Bajpai (2010). Ind. J. Biochem. Biophys.47, 157.

    CAS  Google Scholar 

  63. D. Philip, C. Unni, S. A. Aromal, and V. K. Vidhu (2011). Spectrochim. Acta A78, 899.

    Google Scholar 

  64. M. S. Rad, J. Sharifi, G. A. Heshmati, A. Miri, and D. Jyoti Sen (2013). Am. J. Adv. Drug Deliv.1, 174.

    Google Scholar 

  65. D. Philip (2011). Phys. E43, 1318.

    CAS  Google Scholar 

  66. M. M. H. Khalil, E. H. Ismail, and F. El-Magdoub (2012). Arab. J. Chem.5, 431.

    CAS  Google Scholar 

  67. P. Elia, R. Zach, S. Hazan, S. Kolusheva, Z. Porat, and Y. Zeiri (2014). Int. J. Nanomed.9, 4007.

    Google Scholar 

  68. B. Ankamwar, C. Damle, A. Ahmad, and M. Sastry (2005). J. Nanosci. Nanotechnol.5, 1665.

    CAS  PubMed  Google Scholar 

  69. P. J. Babu, P. Sharma, M. C. Kalita, and U. Bora (2011). Front. Mater. Sci.5, 379.

    Google Scholar 

  70. P. Dauthal and M. Mukhopadhyay (2012). Ind. Eng. Chem. Res.51, 13014.

    CAS  Google Scholar 

  71. D. Raghunandan, S. Basavaraja, B. Mahesh, S. Balaji, S. Manjunath, and A. Venkataraman (2009). Nanobiotechnology5, 34.

    CAS  Google Scholar 

  72. M. Ganeshkumar, M. Sathishkumar, T. Ponrasu, M. G. Dinesh, and L. Suguna (2013). Colloids Surf. B106, 208.

    CAS  Google Scholar 

  73. G. S. Ghodake, N. G. Deshpande, Y. P. Lee, and E. S. Jin (2010). Colloids Surf. B75, 584.

    CAS  Google Scholar 

  74. M. Naruzi, D. Zare, K. Khoshnevisan, and D. Davoodi (2011). Spectrochim. Acta A79, 1461.

    Google Scholar 

  75. S. P. Dubey, M. Lahtinen, and M. Sillanpää (2010). Colloids Surf. A364, 34.

    CAS  Google Scholar 

  76. P. J. Babu, P. Sharma, S. Saranya, R. Tamuli, and U. Bora (2013). Nanomater. Nanotechnol.3, 1.

    Google Scholar 

  77. S. P. Dubey, M. Lahtinen, H. Särkkä, and M. Sillanpää (2010). Colloids Surf. B80, 26.

    CAS  Google Scholar 

  78. S. P. Dubey, M. Lahtinen, and M. Sillanpää (2010). Process Biochem.45, 1065.

    CAS  Google Scholar 

  79. K. Gopinath, S. Gowri, V. Karthika, and A. Arumugam (2014). J. Nanostruct. Chem.4, 115.

    Google Scholar 

  80. K. M. Kumar, B. K. Mandal, M. Sinha, and V. Krishnakumar (2012). Spectrochim. Acta A86, 490.

    Google Scholar 

  81. R. Geethalakshmi and D. L. Sarada (2012). Int. J. Nanomed.7, 5375.

    CAS  Google Scholar 

  82. S. A. Aromal and D. Philip (2012). Spectrochim. Acta A97, 1.

    Google Scholar 

  83. R. Kirubha and G. Alagumuthu (2014). World J. Pharm. Sci.2, 146.

    Google Scholar 

  84. N. Gogna, N. Hamid, and K. Dorai (2015). J.Pharm. Biomed. Anal.10, 115.

    Google Scholar 

  85. J. B. Punuri, P. Sharma, S. Sibyala, R. Tamuli, and U. Bora (2012). Int. Nano Lett.18, 2.

    Google Scholar 

  86. E. Priyadarshini and K. Rawat (2017). J. Mater. Chem. B.5, 27.

    Google Scholar 

  87. M. I. Husseiny, M. A. E. Aziz, Y. Badr, and M. A. Mahmoud (2007). Spectrochim. Acta A Mol. Biomol. Spectrosc.67, 3.

    Google Scholar 

  88. M. Kaasalainen, V. Aseyev, E. Haartman, D. Ş. Karaman, E. Mäkilä, H. Tenhu, J. Rosenholm, and J. Salonen (2017). Nanoscale Res. Lett.12, 74.

    PubMed  PubMed Central  Google Scholar 

  89. X. Xie, J. Liao, X. Shao, Q. Li, and Y. Lin (2017). Sci. Rep.7, 3827.

    PubMed  PubMed Central  Google Scholar 

  90. M. J. Hostetler, E. Wingate, C. J. Zhong, E. Harris, W. Vachet, M. R. Clark, J. D. Londono, S. J. Green, J. J. Stokes, D. Wignall, L. Glish, M. D. Porter, N. D. Evans, and W. Murray (1998). Langmuir82, 1.

    Google Scholar 

  91. J. M. David and X. Hang (2017). NPJ Sci. Food6, 1.

    Google Scholar 

  92. P. Kuppusamy, M. M. Yusoff, G. P. Maniam, and N. Govindan (2016). Saudi Pharm. J.24, 4.

    Google Scholar 

  93. T. L. Botha, E. E. Elemike, S. Horn, D. C. Onwudiwe, J. P. Giesy, and V. Wepener (2019). Sci. Rep.9, 4169.

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors are thankful to Advanced Instrumentation Research Facility (AIRF) and Central Instrumentation Facility (CIF), Jawaharlal Nehru University, New Delhi for providing research facilities. Authors also thank to University Grants Commission (No. F.30-433/2018) for the financial assistance.

Author information

Authors and Affiliations

  1. School of Life Sciences, Sambalpur University, Jyoti Vihar, Odisha, 768019, India

    Biswajit Patra & Surya Narayan Pradhan

  2. School of Environmental Science, Jawaharlal Nehru University, New Delhi, 110067, India

    Biswajit Patra, Rohit Gautam, Eepsita Priyadarsini, Paulraj Rajamani & Ramovatar Meena

  3. Department of Medical Microbiology and Immunology, Division of Biomedical Sciences, School of Medicine, College of Health Sciences, Mekelle University, Tigray, Ethiopia

    Muthupandian Saravanan

Authors
  1. Biswajit Patra
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rohit Gautam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eepsita Priyadarsini
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Paulraj Rajamani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Surya Narayan Pradhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muthupandian Saravanan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ramovatar Meena
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Muthupandian Saravanan or Ramovatar Meena.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Patra, B., Gautam, R., Priyadarsini, E. et al. Piper betle: Augmented Synthesis of Gold Nanoparticles and Its In-vitro Cytotoxicity Assessment on HeLa and HEK293 Cells. J Clust Sci 31, 133–145 (2020). https://doi.org/10.1007/s10876-019-01625-5

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10876-019-01625-5

Keywords

Search

Navigation