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Downregulation of Inflammatory Responses by Novel Caffeic Acid Ester Derivative by Inhibiting NF-kappa B

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Journal of Clinical Immunology Aims and scope Submit manuscript

Abstract

Introduction

Considering anti-tumorigenic activity of caffeic acid phenyl ester, synthesis of several esterified form of caffeic acid is a novel approach in designing for potent drugs.

Results

Our study demonstrates that esterified caffeic acid with methyl vanillate, termed as caffeic acid methyl vanillate ester (CAMVE), blocked inflammatory stimuli-induced inflammatory responses. It decreased amounts of iNOS, Cox-2, and ICAM1 by inhibiting NF-κB through inhibition of IKK activity, IκBα degradation, and p65 nuclear translocation.

Conclusion

Overall, our data suggest that novel caffeic acid ester down-regulates inflammatory responses through inhibition of NF-κB and dependent several gene expressions, further suggesting its efficacy as a promising therapeutic agent.

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Abbreviations

CAMVE:

methyl 4-[{(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoyl}oxy]-3-methoxybenzoate and/or caffeic acid methyl vanillate ester

Cox:

cyclooxygenase

FITC:

fluorescein isothiocyanate

ICAM:

intercellular adhesion molecules

IκBα:

inhibitory subunit of NF-kappaB

IKK:

IκBα kinase

LPS:

lipopolysaccharaide

MFI:

mean fluorescence intensity

NF-κB:

nuclear transcription factor kappa B

Cox:

cyclooxygenase

NE:

nuclear extract

ROI:

reactive oxygen intermediate

SEAP:

secretory alkaline phosphatase

TBARS:

thiobarbituric-acid reactive substances

TNF:

tumor necrosis factor

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Acknowledgments

This work was supported by the Reliance Life Sciences and core grant of Centre for DNA Fingerprinting and Diagnostics (CDFD). We duly thank Dr. J. V. Raman, Research Director and Head, Chemical Research Technology Group, Reliance Life Sciences for valuable comments on the work.

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Authors and Affiliations

  1. Reliance Life Sciences Pvt. Ltd., Mumbai, India

    Julie S. Bose & Vijay Gangan

  2. Department of Biotechnology, Jamia Hamdard, New Delhi, India

    Swatantra Kumar Jain

  3. Laboratory of Immunology, Centre for DNA Fingerprinting and Diagnostics (CDFD), ECIL Road, Nacharam, Hyderabad, India

    Sunil K. Manna

Authors
  1. Julie S. Bose
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  2. Vijay Gangan
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Correspondence to Sunil K. Manna.

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Bose, J.S., Gangan, V., Jain, S.K. et al. Downregulation of Inflammatory Responses by Novel Caffeic Acid Ester Derivative by Inhibiting NF-kappa B. J Clin Immunol 29, 90–98 (2009). https://doi.org/10.1007/s10875-008-9230-3

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  • DOI: https://doi.org/10.1007/s10875-008-9230-3

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