Abstract
In this study, we investigated the effects of arachidonic acid, a PLA2-produced lipid metabolite, on the lysosomal permeability, osmotic sensitivity and stability. Through the measurements of lysosomal β-hexosaminidase free activity, membrane potential, intralysosomal pH, and lysosomal latency loss in hypotonic sucrose medium, we established that arachidonic acid could increase the lysosomal permeability to both potassium ions and protons, and enhance the lysosomal osmotic sensitivity. As a result, the fatty-acid-promoted entry of potassium ions into the lysosomes via K+/H+ exchange, which could produce osmotic imbalance across their membranes and osmotically destabilize the lysosomes. In addition, the enhancement of lysosomal osmotic sensitivity caused the lysosomes to become more liable to destabilization in osmotic shock. The results suggest that arachidonic acid may play a role in the lysosomal destabilization.
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Abbreviations
- CCCP:
-
Carbonyl cyanide m-Chlorophenylhydrazone
- FITC-dextran:
-
Fluorescein isothiocyanate-dextran
- AA:
-
Arachidonic acid
- PLA2 :
-
Phospholipase A2
REFERENCES
Abok K, Hirth T, Ericsson JLE, et al (1983) Virchows Arch (Cell Pathol.) 43: 85–101
Bird SJ, Forster S, Lloyd JB (1987) Biochem J 245: 929–931
Brunk UT, Dalen H, Roberg K, et al (1997) Free Radic Biol Med 23: 616–626
Bruno B, Barbara M, Isabella P, et al (2002) Am J Physiol Cell Physiol 283: C1461–C1468
Casey RP, Hollemans M, Tager JM (1978). Biochim Biophys Acta 508: 15–26
Decker RS, Poole AR, Wildenthal K (1980) Circ Res 46: 485–494
Deter RL, de Duve C (1967) J Cell Biol 33: 437–449
Fell HB, Dingle JT (1963) Biochem J 87: 403–408
Gentili C, Morelli, Susana, et al (2004) J Cell Biochem 93: 312–326
Guskey LE, Smith PC, Wolff DA (1970) J Gen Virol 6: 151–161
Harikumar P, Reeves JP (1983) J Biol Chem 258: 10403–10410
Hatherill JR, Stephens KE, Nagao K, et al (1989) J Biol Resp Mod 8: 614–624
John G (1988) J Membr Biol 106: 83–93
Jonas AJ, Smith ML, Allison WS, et al (1983) J Biol Chem 258: 11727–11730
Laszlo L, Lowe J, Self T, et al (1992) J Pathol 166: 333–341
Lloyd JB, Forster S (1986) Trends Biol Sci 11: 365–368
Loh YP, Tam WWH, Russell JT (1984) J Biol Chem 259: 8238–8245
Lowry OH, Rosebrough NJ, Farr AL, et al (1951) J Biol Chem 193: 265–275
Marchi B, Burlando B, Moore MN, et al (2004) Aquat Toxicol 66: 197–204
Marone G, Fimiani B, Torella G, et al (1983) J Clin Lab Immunol 12: 111–116
Nixon RA, Cataldo AM, Paskevich PA, et al (1992) Ann NY Acad Sci 674: 65–88
Ohkuma S, Moriyama Y, Takano T (1982) Proc Natl Acad Sci USA 79: 2758–2762
Orme FW, Moronne MM, Macey RI (1988) J Membr Biol 104: 57–68
Paula S, Volkov AG, Van Hoek AN, et al (1996) Biophys J 70: 339–348
Piper HM, Sezer O, Schwartz P, et al (1983) Biochim Biophys Acta 732: 193–203
Pjura WJ, Kleinfeld AM, Karnovsky MJ (1984) Biochemistry 23: 2039–2043
Rao AM, Hatcher JF, Kindy MS, et al (1999) Neurochem Res 24: 1225–1232
Reijngoud DJ, Tager JM (1977) Biochim Biophys Acta 472: 419–449
Ruth RC, Weglicki WB (1982) Am J Physiol 242: C192–C199
Takenaka T, Horie H, Hori H, et al (1988) J Membr Biol 106: 141–147
Trauble H (1971) J Membr Biol 4: 193–208
Wan F-Y, Wang Y-N, Zhang G-J (2001) Biochem J 360: 355–362
Wan F-Y, Yang L, Zhong Y-G, et al (2002) Photochem Photobiol 75: 134–139
Wu D, Cederbaum AI (2002) Hepatology 35(6): 1420–1430
Yamada H, Hayashi H, Natori Y (1984) J Biochem 95: 1155–1160
Yang L, Zhang G-J, Zhong Y-G, et al (2000) Cell Biol Int 24: 699–704
Yoshihiro H (2004) J Cell Mol Med 8: 455–464
Zhang G-J, Yao J (1997) Biochim Biophys Acta 1326: 75–82
Zhang G-J, Liu H-W, Yang L, et al (2000) J Membr Biol 175: 53–62
Zhao M, Brunk UT, Eaton JW (2001) FEBS Lett 509: 399–404
Zhong Y-G, Zhang G-J, Yang L, et al (2000) Photochem. Photobiol 71: 627–633
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Zhang, G., Yi, YP. & Zhang, GJ. Effects of Arachidonic Acid on the Lysosomal Ion Permeability and Osmotic Stability. J Bioenerg Biomembr 38, 75–82 (2006). https://doi.org/10.1007/s10863-006-9008-3
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DOI: https://doi.org/10.1007/s10863-006-9008-3