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NMR structure of the HIV-1 reverse transcriptase thumb subdomain

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A Correction to this article was published on 12 December 2017

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Abstract

The solution NMR structure of the isolated thumb subdomain of HIV-1 reverse transcriptase (RT) has been determined. A detailed comparison of the current structure with dozens of the highest resolution crystal structures of this domain in the context of the full-length enzyme reveals that the overall structures are very similar, with only two regions exhibiting local conformational differences. The C-terminal capping pattern of the αH helix is subtly different, and the loop connecting the αI and αJ helices in the p51 chain of the full-length p51/p66 heterodimeric RT differs from our NMR structure due to unique packing interactions in mature RT. Overall, our data show that the thumb subdomain folds independently and essentially the same in isolation as in its natural structural context.

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Change history

  • 12 December 2017

    In the original publication of the article, the given name and family name of the author P. Andrew Karplus was published incorrectly. The name should read as "P. Andrew" – Given name and "Karplus" – Family name.

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Acknowledgements

This work was supported by National Institutes of Health Grants P50GM082251 (to AMG) and R01GM083136 (to PAK). N.G.S was the recipient of a Graduate Research Fellowship, 1247842, from the National Science Foundation.

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Authors and Affiliations

  1. Department of Structural Biology and Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh, School of Medicine, Biomedical Science Tower 3, 3501 Fifth Avenue, Pittsburgh, PA, 15260, USA

    Naima G. Sharaf, In-Ja L. Byeon & Angela M. Gronenborn

  2. Department of Biochemistry and Biophysics, 2011 Ag & Life Sciences Bldg, Oregon State University, Corvallis, OR, 97331, USA

    Andrew E. Brereton & P. Andrew Karplus

Authors
  1. Naima G. Sharaf
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  2. Andrew E. Brereton
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  3. In-Ja L. Byeon
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  4. P. Andrew Karplus
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  5. Angela M. Gronenborn
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Corresponding author

Correspondence to Angela M. Gronenborn.

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A correction to this article is available online at https://doi.org/10.1007/s10858-017-0139-0.

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Sharaf, N.G., Brereton, A.E., Byeon, IJ.L. et al. NMR structure of the HIV-1 reverse transcriptase thumb subdomain. J Biomol NMR 66, 273–280 (2016). https://doi.org/10.1007/s10858-016-0077-2

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  • DOI: https://doi.org/10.1007/s10858-016-0077-2

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