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Accurate micro-computed tomography imaging of pore spaces in collagen-based scaffold

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
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Abstract

In this work we have used X-ray micro-computed tomography (μCT) as a method to observe the morphology of 3D porous pure collagen and collagen-composite scaffolds useful in tissue engineering. Two aspects of visualizations were taken into consideration: improvement of the scan and investigation of its sensitivity to the scan parameters. Due to the low material density some parts of collagen scaffolds are invisible in a μCT scan. Therefore, here we present different contrast agents, which increase the contrast of the scanned biopolymeric sample for μCT visualization. The increase of contrast of collagenous scaffolds was performed with ceramic hydroxyapatite microparticles (HAp), silver ions (Ag+) and silver nanoparticles (Ag-NPs). Since a relatively small change in imaging parameters (e.g. in 3D volume rendering, threshold value and μCT acquisition conditions) leads to a completely different visualized pattern, we have optimized these parameters to obtain the most realistic picture for visual and qualitative evaluation of the biopolymeric scaffold. Moreover, scaffold images were stereoscopically visualized in order to better see the 3D biopolymer composite scaffold morphology. However, the optimized visualization has some discontinuities in zoomed view, which can be problematic for further analysis of interconnected pores by commonly used numerical methods. Therefore, we applied the locally adaptive method to solve discontinuities issue. The combination of contrast agent and imaging techniques presented in this paper help us to better understand the structure and morphology of the biopolymeric scaffold that is crucial in the design of new biomaterials useful in tissue engineering.

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Acknowledgments

This research was carried out under the project CEITEC 2020 (LQ1601) with financial support from the Ministry of Education, Youth and Sports of the Czech Republic under the National Sustainability Programme II.

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Authors and Affiliations

  1. CEITEC-Central European Institute of Technology, Brno University of Technology, Purkynova 123, 61200, Brno, Czech Republic

    Jan Zidek, Lucy Vojtova, A. M. Abdel-Mohsen, Tomas Zikmund, Jana Brtnikova, Lukas Zubal & Jozef Kaiser

  2. Institute of Biomedical Engineering, FEEC, Brno University of Technology, Technicka 12, 61600, Brno, Czech Republic

    Jiri Chmelik, Roman Jakubicek & Jiri Jan

  3. SCITEG, a.s., Brno, Czech Republic

    Lucy Vojtova

  4. Textile Research Division, National Research Centre, El-Buhouth St, P.O. Box 12311, Cairo, Egypt

    A. M. Abdel-Mohsen

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  1. Jan Zidek
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Zidek, J., Vojtova, L., Abdel-Mohsen, A.M. et al. Accurate micro-computed tomography imaging of pore spaces in collagen-based scaffold. J Mater Sci: Mater Med 27, 110 (2016). https://doi.org/10.1007/s10856-016-5717-2

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