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Combined delivery of PDGF-BB and BMP-6 for enhanced osteoblastic differentiation

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Natural microenvironment during bone tissue regeneration involves integration of multiple biological growth factors which regulate mitogenic activities and differentiation to induce bone repair. Among them platelet derived growth factor (PDGF-BB) and bone morphogenic protein-6 (BMP-6) are known to play a prominent role. The aim of this study was to investigate the benefits of combined delivery of PDGF-BB and BMP-6 on proliferation and osteoblastic differentiation of MC3T3-E1 preosteoblastic cells. PDGF-BB and BMP-6 were loaded in gelatin and poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) particles, respectively. The carrier particles were then loaded into 3D chitosan matrix fabricated by freeze drying. The fast release of PDGF-BB during 7 days was accompanied by slower and prolonged release of BMP-6. The premising release of mitogenic factor PDGF-BB resulted in an increased MC3T3-E1 cell population seeded on chitosan scaffolds. Osteogenic markers of RunX2, Col 1, OPN were higher on chitosan scaffolds loaded with growth factors either individually or in combination. However, OCN expression and bone mineral formation were prominent on chitosan scaffolds incorporating PDGF-BB and BMP-6 as a combination.

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Acknowledgments

This study is supported by Ankara University Research Foundation (Project No: 11B4343006).

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    Authors and Affiliations

    1. Bioengineering Department, Hacettepe University, Beytepe, 06800, Ankara, Turkey

      T. Tolga Demirtaş & Menemşe Gümüşderelioğlu

    2. Chemical Engineering Department, Faculty of Engineering, Ankara University, 06100, Ankara, Turkey

      Eda Göz & Ayşe Karakeçili

    3. Chemical Engineering Department, Hacettepe University, Beytepe, 06800, Ankara, Turkey

      Menemşe Gümüşderelioğlu

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    1. T. Tolga Demirtaş
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    2. Eda Göz
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    Correspondence to Ayşe Karakeçili.

    Additional information

    T. Tolga Demirtaş and Eda Göz have equally contributed to this study.

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    10856_2015_5626_MOESM1_ESM.jpg

    Supplementary material 1 SEM images of crosslinked gelatin microparticles prepared by water-in-oil emulsion method a) × 2000, b) x750 and c) x500. The diameter of the particles was 50-100 μm (JPEG 35 kb)

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    Demirtaş, T.T., Göz, E., Karakeçili, A. et al. Combined delivery of PDGF-BB and BMP-6 for enhanced osteoblastic differentiation. J Mater Sci: Mater Med 27, 12 (2016). https://doi.org/10.1007/s10856-015-5626-9

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    • DOI: https://doi.org/10.1007/s10856-015-5626-9

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