Skip to main content
SpringerLink
Log in

Short-term effect of magnesium implantation on the osteomyelitis modeled animals induced by Staphylococcus aureus

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Pure magnesium (Mg) granules were implanted into the tibial medullary cavity of osteomyelitis modeled animals after debridement, and the animals without implant were taken as the control group. The antibacterial and osteogenic effects on bone tissue during Mg degradation were evaluated through detecting Mg ions, counting bacteria culture in peripheral blood, histology and iconography. The results showed that there was no significant difference for the concentration of serum Mg between the preoperative and postoperative animals within 5 weeks, maintaining in the normal range, and the number of bacteria in bone tissue of the Mg implant group was significantly lower than that of the control group. Mg implantation showed good biocompatibility no harmful to the liver, spleen, kidney and other organs in the modeled animals. In addition, the formation rate of new bone tissues around the implanted Mg was faster, indicating that the degradation of Mg could also promote the osteogenic process with good biocompatibility.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10

Similar content being viewed by others

References

  1. Schmidt-Rohlfing B, Lemmen SW, Pfeifer R, et al. Osteomyelitis in adults: diagnostic principles and therapeutic strategies. Unfallchirurg. 2012;115:55–66.

    Article  CAS  Google Scholar 

  2. Geng F, Tan LL, Zhang BC, et al. Study on β-TCP coated porous Mg as a bone tissue engineering scaffold material. J Mater Sci Technol. 2009;25:123–9.

    CAS  Google Scholar 

  3. Ren YB, Huang JJ, Yang K, et al. Study of bio-corrosion of pure magnesium. Acta Metall Sin. 2005;41:1228–32.

    CAS  Google Scholar 

  4. Saris NE, Mervaala E, Karppanen H, et al. Magnesium: an update on physiological, clinical and analytical aspects. Clin Chim Acta. 2000;294:1–26.

    Article  CAS  Google Scholar 

  5. Witte F, Kaese V, Haferkamp H, et al. In vivo corrosion of four magnesium alloys and the associated bone response. Biomaterials. 2005;26:3557–63.

    Article  CAS  Google Scholar 

  6. Witte F, Ulrich H, Rudert M, et al. Biodegradable magnesium scaffolds part I: appropriate inflammatory response. J Biomed Mater Res A. 2007;81:748–56.

    CAS  Google Scholar 

  7. Witte F, Ulrich H, Palm C, et al. Biodegradable magnesium scaffolds part II: peri-implant bone remodeling. J Biomed Mater Res A. 2007;81:757–65.

    CAS  Google Scholar 

  8. Janning C, Willbold E, Vogt C, et al. Magnesium hydroxide temporarily enhancing osteoblast activity and decreasing the osteoclast number in peri-implant bone remodelling. Acta Biomater. 2010;6:l861–8.

    Article  Google Scholar 

  9. Abed E, Moreau R. Importance of melastatin-like transient receptor potential 7 and magnesium in the stimulation of osteoblast proliferation and migration by platelet-derived growth factor. Am J Physiol Cell Physiol. 2009;297:C360–8.

    Article  CAS  Google Scholar 

  10. Wolf FI, Cittadini A. Chemistry and biochemistry of magnesium. Mol Aspects Med. 2003;24:3–9.

    Article  CAS  Google Scholar 

  11. Sacks FM, Willett WC, Smith A, et al. Effect on blood pressure of potassium, calcium and magnesium in women with low habitual intake. Hypertension. 1998;31:131–8.

    Article  CAS  Google Scholar 

  12. Vormann J. Magnesium: nutrition and metabolism. Mol Aspects Med. 2003;24:27–37.

    Article  CAS  Google Scholar 

  13. Gao JC, Wu S, Qiao LY, et al. Corrosion behavior of Mg and Mg-Zn alloys in simulated body fluid. Transactions of Nonferrous Metals Society of China. 2008;18:588–92.

    Article  CAS  Google Scholar 

  14. Xin YC, Jiang J, Huo KF, et al. Corrosion resistance and cytocompatibility of biodegradable surgical magnesium alloy coated with hydrogenated amorphous silicon. J Biomed Mater Res Part A. 2009;89:717–26.

    Article  Google Scholar 

  15. Slottow TL, Pakala R, Okabe T, et al. Optical coherence tomography and intravascular ultrasound imaging of bioabsorbable magnesium stent degradation in porcine coronary arteries. Cardiovasc Revasc Med. 2008;9:248–54.

    Article  Google Scholar 

  16. Robinson DA, Griffith RW, Shechtman D, et al. In vitro antibacterial properties of magnesium metal against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Acta Biomater. 2010;6:1869–77.

    Article  CAS  Google Scholar 

  17. Ren Ling, Lin Xiao, Tan Lili, et al. Effect of surface coating on antibacterial behavior of magnesium based metals. Mater Lett. 2011;65:3509–11.

    Article  CAS  Google Scholar 

  18. White D. Membrane bioenergetics: the proton potential. In: The physiology and biochemistry of prokaryotes, 3rd ed. New York: Oxford University Press; 2007.p.83–119.

  19. Padan E, Bibi E, Ito M, et al. Alkaline pH homeostasis in bacteria: new insights. Biochim Biophys Acta. 2005;1717:67–88.

    Article  CAS  Google Scholar 

  20. Hallab NJ, Vermes C, Messina C, et al. Concentration- and composition-dependent effects of metal ions on human MG-63 osteoblasts. J Biomed Res. 2002;A60:420–33.

    Article  Google Scholar 

  21. Kuwahara H, Al-Abdullat Y, Mazald N, Tsutsumi S, et al. Precipitation of magnesium apatite on pure magnesium surface during immersing in Hank’s solution. Materials Transactions. 2001;42:1317–21.

    Article  CAS  Google Scholar 

  22. Witte F, Feyerabend F, Maier P, et al. Biodegradable magnesium-hydroxyapatite metal matrix composites. Biomaterials. 2007;28:2163–74.

    Article  CAS  Google Scholar 

  23. Chen ZH, Yan HG, Chen JH, et al. Magnesium Alloys[M]. Beijing: Chemical Industry Press; 2004. p. 386.

    Google Scholar 

  24. Mader JT, Stevens CM, Stevens JH, et al. Treatment of experimental osteomyelitis with a fibrin sealant antibiotic implant. Clin Orthop Relat Res. 2002;403:58–72.

    Article  Google Scholar 

  25. Minpeng Lu, Jiang Dianming, Quan Zhengxue, et al. The establishment of the rabbit tibia chronic osteomyelitis animal model. Chin J Exp Surg. 2010;27:664–6.

    Google Scholar 

  26. Liu Feng, Wang Baogen, Zhu Shutao, et al. Experimental animals and trace elements I: Determination of the six elements in the New Zealand white rabbit serum. Shanghai Lab Anim Sci. 1987;7:234.

    Google Scholar 

  27. Zhang Guangdao, Huang Jingjing, Yang Ke, et al. Experimental study of in vivo implantation of a magnesium alloy at early stage. Acta Metall Sin. 2007;43:1186–90.

    CAS  Google Scholar 

  28. Chen HW, Huang HB, Zhao GS, et al. Treatment of chronic osteomyelitis with antibiotic-loaded calcium phosphate cement. Zhongguo Gu Shang. 2008;21:79–81.

    CAS  Google Scholar 

  29. Tang H, Xu Y, Li G, et al. Treatment of chronic osteomyelitis of rabbit with liposomal gentamicin-impregnated allogeneic cortical bone. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010;24:482–6.

    Google Scholar 

  30. Shi P, Zuo Y, Li X, et al. Gentamicin-impregnated chitosan/nanohydroxyapatite/ethyl cellulose microspheres granules for chronic osteomyelitis therapy. J Biomed Mater Res A. 2010;93:1020–31.

    Google Scholar 

  31. Jiang JL, Li YF, Fang TL, et al. Vancomycin-loaded nano-hydroxyapatite pellets to treat MRSA-induced chronic osteomyelitis with bone defect in rabbits. Inflamm Res. 2012;61:207–15.

    Article  CAS  Google Scholar 

  32. Chen D, Harris MA, Rossini G, et al. Bone morphogentic protein 2 enhances BMP-3, BMP-4, and bone cell differentiation maker gene expression during the induction of mineralized bone matrix formation in cultures of fetal rat calvarial osteoblasts. Calcif Tissue Int. 1997;60:283–90.

    Article  CAS  Google Scholar 

  33. Urist MR, Strates BS. The classic: bone morphogenetic protein. Clin Orthop Relat Res. 2009;467:3051–62.

    Article  Google Scholar 

  34. Chen S, Guttridge DC, Tang E, et al. Suppression of Tumor Necrosis Factor-mediated Apoptosis by Nuclear Factor kB-independent Bone Morphogenetic Protein/Smad Signaling. J Biol Chem. 2001;276:39259–63.

    Article  CAS  Google Scholar 

  35. Liu Z, Shi W, Ji X, et al. Molecules mimicking Smad1 interacting with Hox stimulate bone formation. J Biol Chem. 2004;279:11313–9.

    Article  CAS  Google Scholar 

  36. Tachi K, Takami M, Sato H, et al. Enhancement of bone morphogenetic protein-2-induced ectopic bone formation by transforming growth factor-β1. Tissue Eng Part A. 2011;17:597–606.

    Article  CAS  Google Scholar 

  37. Zhang E, Xu L, Yu G, et al. In vivo evaluation of biodegradable magnesium alloy bone implant in the first 6 months implantation. J Biomed Mater Res A. 2009;90:882–93.

    Google Scholar 

  38. Chen D, He Y, Tao H, et al. Biocompatibility of magnesium-zinc alloy in biodegradable orthopedic implants. Int J Mol Med. 2011;28:343–8.

    CAS  Google Scholar 

  39. Kraus T, Fischerauer SF, Hänzi AC, et al. Magnesium alloys for temporary implants in osteosynthesis: in vivo studies of their degradation and interaction with bone. Acta Biomater. 2012;8:1230–8.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by funds from Higher Excellent Talents Support Program of Liaoning Province (NO. LJQ2011089) and a National Basic Research Program (No.2012CB619101).

Author information

Authors and Affiliations

  1. Department of Orthopedics, First Affiliated Hospital of Liaoning Medical University, No. 5-2, Renmin Street, Guta District, Jinzhou, 121000, China

    Jinhao Zeng, Yajiang Yuan, Yansong Wang, Jianbo Zhao & Xifan Mei

  2. Institute of Metal Research, Chinese Academy of Sciences, 72 Wenhua Road, Shenyang, 110016, China

    Ling Ren & Ke Yang

  3. Shenzhen Prevention and Treatment Center for Occupational Diseases, 70 Guiyuan North Road, Shenzhen, 518001, China

    Ruming Zeng

Authors
  1. Jinhao Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ling Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yajiang Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yansong Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jianbo Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ruming Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ke Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xifan Mei
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Ke Yang or Xifan Mei.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zeng, J., Ren, L., Yuan, Y. et al. Short-term effect of magnesium implantation on the osteomyelitis modeled animals induced by Staphylococcus aureus . J Mater Sci: Mater Med 24, 2405–2416 (2013). https://doi.org/10.1007/s10856-013-4982-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-013-4982-6

Keywords

Search

Navigation