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Laser pulse dependent micro textured calcium phosphate coatings for improved wettability and cell compatibility

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Abstract

Surface wettability of an implant material is an important criterion in biological response as it controls the adsorption of proteins followed by attachment of cells to its surface. Hence, micro-textured calcium phosphate coatings with four length scales were synthesized on Ti–6Al–4V substrates by a laser cladding technique and their effects on wettability and cell adhesion were systematically evaluated. Microstructure and morphological evolutions of the coatings were studied using scanning electron and light optical microscopes respectively. The surface texture of coating defined in terms of a texture parameter was correlated to its wetting behavior. The contact angle of simulated body fluid measured by a static sessile drop technique, demonstrated an increased hydrophilicity with decreasing value of texture parameter. The influence of such textures on the in vitro bioactivity and in vitro biocompatibility were studied by the immersion of the samples in simulated body fluid and mouse MC3T3-E1 osteoblast-like cell culture respectively.

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Acknowledgments

The authors would like to acknowledge Dr. John Dunlop of University of Tennessee, Knoxville in helping us preparing the samples for SEM observations, Lu Huang for cell culture, and Dr. Peter Liaw of University of Tennessee, Knoxville in providing the cell line.

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Authors and Affiliations

  1. Department of Materials Science and Engineering, The University of Tennessee, Knoxville, TN, 37996, USA

    Sameer R. Paital, Wei He & Narendra B. Dahotre

  2. Department of Mechanical, Aerospace and Biomedical Engineering, The University of Tennessee, Knoxville, TN, 37996, USA

    Wei He

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  1. Sameer R. Paital
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  2. Wei He
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Correspondence to Narendra B. Dahotre.

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Paital, S.R., He, W. & Dahotre, N.B. Laser pulse dependent micro textured calcium phosphate coatings for improved wettability and cell compatibility. J Mater Sci: Mater Med 21, 2187–2200 (2010). https://doi.org/10.1007/s10856-010-4085-6

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  • DOI: https://doi.org/10.1007/s10856-010-4085-6

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