Abstract
The mechanical properties of bone are dictated by the size, shape and organization of the mineral and matrix phases at multiple levels of hierarchy. While much is known about structure–function relations at the macroscopic level, less is known at the nanoscale, especially for trabecular bone. In this study, high resolution transmission electron microscopy (HRTEM) was carried out to analyze shape and orientation of apatite crystals in murine femoral trabecular bone. The distribution and orientation of mineral apatites in trabecular bone were different from lamellar bone and the c-axis of the tablet-like mineral apatite crystals in trabecular bone was arranged with no preferred orientation. The difference in the orientation distribution of apatite crystals of trabecular bone in the present study compared with that of lamellar bone found in the literature can be attributed to the more complex local stress state in trabecular bone. Apatite crystals were also found to be multi-crystalline, not single crystalline, from dark field image analysis. From the observations of this study, it is suggested that Wolff’s law can be applicable to the nanostructural orientation and distribution of apatite crystals in trabecular bone. It was also found that small round crystalline particles observed adjacent to collagen fibrils were similar in size and shape to the apatite crystals in biomimetically nucleated synthetic amorphous calcium phosphate, which suggests that they are bone mineral apatite nuclei.
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References
Hancox NM. Biology of bone. Cambridge: Cambridge University Press; 1972. p. 3–48.
Landis WJ, Song MJ, Leith A, McEwen L, McEwen BF. Mineral and organic interaction in normally calcifying tendon visualized in three dimensions by high voltage electron microscopic tomography and graphic imaging reconstruction. J Struct Biol. 1993;110:39–54. doi:10.1006/jsbi.1993.1003.
Rho JY, Kuhn-Spearing L, Zioupos P. Mechanical properties and the hierarchial structure of bone. Med Eng Phys. 1998;20:92–102.
Su X, Sun K, Cui FZ, Landis WJ. Organization of apatite crystals in human woven bone. Bone. 2003;32:150–62.
Rohanizadeh R, LeGeros RZ, Bohie S, Pilet P, Barbier A, Daculsi G. Ultrastructural properties of bone mineral of control and tiludronate-treated osteoporotic rat. Calcif Tissue Int. 2000;67:330–6. doi:10.1007/s002230001141.
Rubin MA, Jasiuk I, Taylor J, Rubin J, Ganey T, Apkarian RP. TEM analysis of the nanostructure of normal and osteoporotic human trabecular bone. Bone. 2003;33:270–82. doi:10.1016/S8756-3282(03)00194-7.
Martin RB, Burr DB, Sharkey NA. Skeletal tissue mechanics. New York: Springer; 1998. p. 227–30.
Rindby A, Voglis P, Engstrom P. Microdiffraction studies of bone tissues using synchrotron radiation. Biomaterials. 1998;19:2083–92. doi:10.1016/S0142-9612(98)00120-3.
Chen QZ, Wong CT, Lu WW, Cheung KMC, Leong JCY, Luk KDK. Strengthening mechanism of bone bonding to crystalline hydroxyapatite in vivo. Biomaterials. 2004;25:4234–54. doi:10.1016/j.biomaterials.2003.11.017.
Hohling HJ. Collagen mineralization in bone, dentine, cementum and cartilage. Naturwissenschaften. 1969;56:466–76. doi:10.1007/BF00601080.
Weiner S, Wagner HD. The material bone: structure-mechanical function relations. Annu Rev Mater Sci. 1998;28:271–98. doi:10.1146/annurev.matsci.28.1.271.
Landis WJ. The strength of a calcified tissue depends in part on the molecular structure and organization of its constituent mineral crystals in their organic matrix. Bone. 1995;16:533–44. doi:10.1016/8756-3282(95)00076-P.
Sahar ND, Hong SI, Kohn DH. Micro- and nano-structural analyses in bone. Micron. 2005;36:617–29. doi:10.1016/j.micron.2005.07.006.
Khan K, McKay H, Kannus P, Bailey D, Wark J, Bennel K. Physical activity and bone health. Champaign: Human Kinetics; 2001. p. 16.
Jena AK, Chaturvedi MC. Phase transformation in materials. Englewood Cliffs: Prentice Hall; 1992. p. 151, 302.
Hong SI, Gray GT, Wang Z. Microstructure and stress-strain response of Al-Mg-Si alloy composites reinforced with 15% Al2O3. Mater Sci Eng. 1996;221:38–47. doi:10.1016/S0921-5093(96)10483-4.
Hong SI, Lee KH, Outslay M, Kohn DH. Ultrastructural analyses of nanoscale apatite biomimetically grown on organic template. J Mater Res. 2008;23:478–85. doi:10.1557/jmr.2008.0051.
Sasaki N, Sudoh Y. X-ray pole figure analysis of apatite crystals and collagen molecules in bone. Calcif Tissue Int. 1997;60:361–7. doi:10.1007/s002239900244.
Luong LN, Hong SI, Patel RJ, Outslay ME, Kohn DH. Spatial control of protein within biomimetically nucleated mineral. Biomaterials. 2006;27:1175–84. doi:10.1016/j.biomaterials.2005.07.043.
Cuisinier FJG, Steuer P, Brisson A, Voegel JC. High resolution electron microscopy of crystal growth mechanisms in chicken bone composites. J Cryst Growth. 1995;156:443–53. doi:10.1016/0022-0248(95)00237-5.
Acknowledgment
We acknowledge the support from US DoD/Dept. of the Army DAMD17-03-1-0556. SIH is grateful for the support from Korea Research Foundation (D00318).
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Hong, S.I., Hong, S.K. & Kohn, D.H. Nanostructural analysis of trabecular bone. J Mater Sci: Mater Med 20, 1419–1426 (2009). https://doi.org/10.1007/s10856-009-3708-2
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DOI: https://doi.org/10.1007/s10856-009-3708-2