Abstract
In a comprehensive picture of inclusion complex formation of the highly fluorescent dye coumarin-6 (C6) and betacyclodextrin (beta-CD), which was obtained using various fluorescence spectroscopic methods, it was demonstrated that up to three beta-CD rings can thread on the rod like dye molecule. Interaction of coumarins and modified coumarins with cellular organelles or proteins has been reported in several publications. Especially 7-amino-coumarins are characterized by unique properties like high fluorescence quantum yield and are thus already used successfully in different areas, like staining of fluorescent nanoparticles. We could show that Coumarin-6 made soluble by complexation with beta-cyclodextrin is able to stain eukaryotic cells specifically dependent on their origin and cellular behaviour. The staining reaction is independent from pH, is photo stable, and shows no cross talk with proteins in the cytoplasm and other staining procedures or erythrocytes. Staining with coumarin 6/cyclodextrin complexes can thus be used for fast discrimination of different cell types. Importantly, it could be shown that the ideal staining reaction is dependent on the stoichiometry of the complex-formation.
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Michael Edetsberger and Martin Knapp are the authors contributed equally.
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Edetsberger, M., Knapp, M., Gaubitzer, E. et al. Effective staining of tumor cells by coumarin-6 depends on the stoichiometry of cyclodextrin complex formation. J Incl Phenom Macrocycl Chem 70, 327–331 (2011). https://doi.org/10.1007/s10847-010-9894-1
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DOI: https://doi.org/10.1007/s10847-010-9894-1