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Model-based asessment of an in-vivo predictive relationship from CA1 to CA3 in the rodent hippocampus

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Abstract

Although an anatomical connection from CA1 to CA3 via the Entorhinal Cortex (EC) and through backprojecting interneurons has long been known it exist, it has never been examined quantitatively on the single neuron level, in the in-vivo nonpatholgical, nonperturbed brain. Here, single spike activity was recorded using a multi-electrode array from the CA3 and CA1 areas of the rodent hippocampus (N = 7) during a behavioral task. The predictive power from CA3→CA1 and CA1→CA3 was examined by constructing Multivariate Autoregressive (MVAR) models from recorded neurons in both directions. All nonsignificant inputs and models were identified and removed by means of Monte Carlo simulation methods. It was found that 121/166 (73 %) CA3→CA1 models and 96/145 (66 %) CA1→CA3 models had significant predictive power, thus confirming a predictive ’Granger’ causal relationship from CA1 to CA3. This relationship is thought to be caused by a combination of truly causal connections such as the CA1→EC→CA3 pathway and common inputs such as those from the Septum. All MVAR models were then examined in the frequency domain and it was found that CA3 kernels had significantly more power in the theta and beta range than those of CA1, confirming CA3’s role as an endogenous hippocampal pacemaker.

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Acknowledgments

This work was supported by NIH grant P41-EB001978 to the Biomedical Simulations Resource at the University of Southern California and DARPA contract N66601-09-C-2081.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Southern California, DRB 367, 1042 Downey Way Los Angeles, Los Angeles, CA, 90089, USA

    Roman A. Sandler, Dong Song, Robert E. Hampson, Sam A. Deadwyler, Theodore W. Berger & Vasilis Z. Marmarelis

  2. Department of Physiology and Pharmacology, Wake Forest School of Medicine, Winston-Salem, NC, USA

    Robert E. Hampson & Sam A. Deadwyler

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  1. Roman A. Sandler
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  2. Dong Song
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  3. Robert E. Hampson
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  4. Sam A. Deadwyler
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  5. Theodore W. Berger
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  6. Vasilis Z. Marmarelis
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Correspondence to Roman A. Sandler.

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Sandler, R.A., Song, D., Hampson, R.E. et al. Model-based asessment of an in-vivo predictive relationship from CA1 to CA3 in the rodent hippocampus. J Comput Neurosci 38, 89–103 (2015). https://doi.org/10.1007/s10827-014-0530-8

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