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Embryonic poly(A)-binding protein interacts with translation-related proteins and undergoes phosphorylation on the serine, threonine, and tyrosine residues in the mouse oocytes and early embryos

  • Embryo Biology
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Abstract

Expression of the embryonic poly(A)-binding protein (EPAB) in frog, mouse, and human oocytes and early-stage embryos is maintained at high levels until embryonic genome activation (EGA) after which a significant decrease occurs in EPAB levels. Studies on the vertebrate oocytes and early embryos revealed that EPAB plays key roles in the translational regulation, stabilization, and protection of maternal mRNAs during oocyte maturation and early embryogenesis. However, it remains elusive whether EPAB interacts with other cellular proteins and undergoes phosphorylation to perform these roles. For this purpose, we identified a group of Epab-interacting proteins and its phosphorylation status in mouse germinal vesicle (GV)- and metaphase II (MII)-stage oocytes, and in 1-cell, 2-cell, and 4-cell preimplantation embryos. In the oocytes and early preimplantation embryos, Epab-interacting proteins were found to play roles in the translation and transcription processes, intracellular signaling and transport, maintenance of structural integrity, metabolism, posttranslational modifications, and chromatin remodeling. Moreover, we discovered that Epab undergoes phosphorylation on the serine, threonine, and tyrosine residues, which are localized in the RNA recognition motifs 2, 3, and 4 or C-terminal. Conclusively, these findings suggest that Epab not only functions in the translational control of maternal mRNAs through binding to their poly(A) tails but also participates in various cellular events through interacting with certain group proteins. Most likely, Epab undergoes a dynamic phosphorylation during the oocyte maturation and the early embryo development to carry out these functions.

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Data availability

The datasets generated during the current study are available from the corresponding author on request.

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Funding

This study was supported by TUBITAK (grant no. 218S612).

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Authors and Affiliations

  1. Department of Histology and Embryology, Akdeniz University School of Medicine, Campus, Antalya, 07070, Turkey

    Saffet Ozturk, Esra Gozde Kosebent, Gunel Talibova, Yesim Bilmez & Betul Tire

  2. Department of Histology and Embryology, Ankara University School of Medicine, Ankara, 06410, Turkey

    Alp Can

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  1. Saffet Ozturk
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Correspondence to Saffet Ozturk.

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All experimental protocols were performed in accordance with relevant guidelines and regulations approved by the Akdeniz University Institutional Animal Care and Use Committee (protocol number: 1286/2021.04.011).

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Ozturk, S., Kosebent, E.G., Talibova, G. et al. Embryonic poly(A)-binding protein interacts with translation-related proteins and undergoes phosphorylation on the serine, threonine, and tyrosine residues in the mouse oocytes and early embryos. J Assist Reprod Genet 40, 929–941 (2023). https://doi.org/10.1007/s10815-023-02746-7

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  • DOI: https://doi.org/10.1007/s10815-023-02746-7

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