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The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage

  • Fertility Preservation
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Abstract

Purpose

We evaluated the protective effect of PRP on ovarian function in female rats with cyclophosphamide (Cy)-induced ovarian damage.

Methods

Thirty-two adult female Sprague–Dawley rats were randomly divided into four groups. Group 1 (control-sodium chloride 0.9%; 1 mL/kg, single-dose ip injection), group 2 (Cy); 75 mg/kg, single-dose ip injection and sodium chloride 0.9% (1 mL/kg, single-dose ip injection), group 3 Cy plus PRP, Cy (75 mg/kg, single-dose and PRP (200 μl, single-dose) ip injection), group 4 (PRP, 200 μl, single-dose ip injection). Primordial, antral, and atretic follicle counts; serum anti-Müllerian hormone (AMH) levels; AMH-positive granulosa cells; and gene expression analysis of Ddx4 were assessed.

Results

Serum AMH levels were significantly lower in group 2 compared to groups 1, 3, and 4 (p < 0.01, p < 0.01, and p = 0.04, respectively). A significant difference was found in the primordial, primary, secondary, antral, and atretic follicle counts between all groups (p < 0.01). There was a statistically significant difference in AMH-positive staining primary, secondary, and antral follicles count between the groups (p < 0.01). There was a statistically significant difference in primary, secondary, and antral AMH positive staining follicle intensity score between the groups (p < 0.01). Ddx4 expression in group 4 was highest compared to other groups.

Conclusion

Our study may provide evidence that PRP could protect ovarian function against ovarian damage induced by Cy. It could lead to improved primordial, primary, secondary, and antral follicle numbers.

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Author information

Author notes

  1. Pinar Ozcan

    Present address: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Bezmialem University Faculty of Medicine, İstanbul, Turkey

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Bezmialem University Faculty of Medicine, İskender Paşa Mh Adnan Menderes Bulvarı, Vatan Cad, 34093, Fatih/İstanbul, Turkey

    Pinar Ozcan, Taha Takmaz & Sevde Islek

  2. Department of Histology and Embryology, Faculty of Medicine, Istanbul Medipol University, İstanbul, Turkey

    Olgu Enis Tok

  3. Institute of Biotechnology, Gebze Technical University, Kocaeli, Turkey

    Esra Nur Yigit

  4. Department of Obstetrics and Gynecology, Acibadem Health Group, Istanbul, Turkey

    Cem Ficicioglu

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  1. Pinar Ozcan
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Correspondence to Pinar Ozcan.

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Ozcan, P., Takmaz, T., Tok, O.E. et al. The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage. J Assist Reprod Genet 37, 865–873 (2020). https://doi.org/10.1007/s10815-020-01689-7

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  • DOI: https://doi.org/10.1007/s10815-020-01689-7

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