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Micro-RNAs involved in cellular proliferation have altered expression profiles in granulosa of young women with diminished ovarian reserve

  • REPRODUCTIVE PHYSIOLOGY AND DISEASE
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

The study aims to determine differences in micro-RNA (miRNA) expression in granulosa (GC) and cumulus cells (CC) between young women with diminished ovarian reserve (DOR) or normal ovarian reserve (NOR). Secondary objective was to identify downstream signaling pathways that could ultimately indicate causes of lower developmental competence of oocytes from young women with DOR.

Methods

The method of the study is prospective cohort study.

Results

Of the miRNA, 125 are differentially expressed in GC between DOR and NOR. Only nine miRNA were different in CC; therefore, we focused analysis on GC. In DOR GC, miR-100-5p, miR-16-5p, miR-30a-3p, and miR-193a-3p were significantly downregulated, while miR-155-5p, miR-192-5p, miR-128-3p, miR-486-5p, miR130a-3p, miR-92a-3p, miR-17-3p, miR-221-3p, and miR-175p were increased. This pattern predicted higher cell proliferation in the DOR GC. The primary pathways include MAPK, Wnt, and TGFbeta.

Conclusions

The miRNA pattern identified critical functions in cell proliferation and survival associated with DOR. GC in women with DOR seems to respond differently to the LH surge.

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Acknowledgements

The research was supported by NIH grant HD082484 awarded to LKM and LKC.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, Keck School of Medicine, University of Southern California, 2020 Zonal Avenue, IRD 534, Los Angeles, CA, 90033, USA

    Irene Woo, Sue Ann Ingles, Semara Thomas, Ali Ahmady, Karine Chung, Kristin Bendikson, Richard Paulson & Lynda K. McGinnis

  2. Department of Molecular and Integrative Physiology, University of Kansas Medical Center, Kansas City, KS, USA

    Lane K. Christenson & Sumedha Gunewardena

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  1. Irene Woo
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  2. Lane K. Christenson
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  3. Sumedha Gunewardena
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  10. Lynda K. McGinnis
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Correspondence to Lynda K. McGinnis.

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Woo, I., Christenson, L.K., Gunewardena, S. et al. Micro-RNAs involved in cellular proliferation have altered expression profiles in granulosa of young women with diminished ovarian reserve. J Assist Reprod Genet 35, 1777–1786 (2018). https://doi.org/10.1007/s10815-018-1239-9

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  • DOI: https://doi.org/10.1007/s10815-018-1239-9

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