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Expression of a Heat Shock Protein 70 from the Brown Alga Ectocarpus sp. Imparts Salinity Stress Tolerance in Arabidopsis thaliana

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Abstract

The brown alga Ectocarpus sp. belongs to Phaeophyceae, a class of macroalgae in the phylum Heterokonts, which is also known as the Stramenopiles lineage. Ectocarpus is a dominant seaweed in temperate regions around the globe, abundant mostly in the intertidal zone, a habitat with harsh environmental conditions resulting from tidal cycles. Analysis of previously generated transcriptomic data of brown alga showed the up-regulation of the Esi0379_0027 gene, encoding a HSP70 protein, in response to various abiotic stresses. Bioinformatics study demonstrated that the HSP70 protein is soluble, monomeric and well conserved compared to other organisms. Subcellular localization of EsHSP70 revealed that the protein is localized in the nucleus. To explore the role of HSP70 in stress tolerance, it was expressed in Arabidopsis under constitutive and stress inducible promoters. Transgenic Arabidopsis plants generated using both promoters exhibited higher tolerance to salinity stress compared to wild type plant. Transcript analysis of various abiotic stress-responsive genes showed that genes participating in Na+ efflux and sequestration, and in ABA-mediated stress tolerance were remarkably upregulated in the EsHSP70-expressing transgenic Arabidopsis. Overall, our results showed that the expression of the EsHSP70 promotes salt stress tolerance by upregulating stress responsive genes in Arabidopsis.

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Data are available for the authors upon reasonable request.

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Funding

B.P.’s lab was supported by NSERC-DG grant 1177546, MITACS and France-Canada Research Fund (FCRF). P.R. was supported by an NSERC Industrial Postgraduate Scholarship and a MITACS Globalink Research Award. P.P. and T.T. were also supported by the Agence Nationale de la Recherche via the investment expenditure program IDEALG Grant ANR-10-BTBR-04.

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  1. Marine Bio-Products Research Laboratory, Department of Plant, Food and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Nova Scotia, NS, B2N5E3, Canada

    Pramod Rathor, Tudor Borza, Ramin Bahmani & Balakrishnan Prithiviraj

  2. Department of Biology, Dalhousie University, Halifax, NS, B3H 4R2, Canada

    Sophia Stone

  3. Centre for Novel Agricultural Products, Department of Biology, University of York, Heslington, YO10 5DD, York, UK

    Thierry Tonon

  4. Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff (SBR), Sorbonne Université, CNRS, UMR 8227, 29680, Roscoff, France

    Thierry Tonon & Philippe Potin

  5. Grain Legume Genetics and Physiology Research Unit, USDA-ARS, Prosser, WA, 99350, USA

    Svetlana Yurgel

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Contributions

Pramod Rathor: Conceptualization, methodology, validation, formal analysis, investigation, data curation, writing—original draft preparation, writing—review and editing, visualization. Tudor Borza: Conceptualization, methodology, validation, formal analysis, investigation, data curation, writing—original draft preparation, writing—review and editing, visualization. Ramin Bahmani: formal analysis, investigation, validation, visualization, writing – review & editing. Sophia Stone: writing – review & editing. Thierry Tonon: resources, writing—review and editing, funding acquisition. Svetlana Yurgel: Writing – review & editing. Philippe Potin: resources, writing—review and editing, funding acquisition. Balakrishnan Prithiviraj: Conceptualization, methodology, supervision, project administration, writing—review and editing, funding acquisition, resources.

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Correspondence to Balakrishnan Prithiviraj.

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Rathor, P., Borza, T., Bahmani, R. et al. Expression of a Heat Shock Protein 70 from the Brown Alga Ectocarpus sp. Imparts Salinity Stress Tolerance in Arabidopsis thaliana. J Appl Phycol 35, 803–819 (2023). https://doi.org/10.1007/s10811-022-02897-7

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