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Changes on the intestinal bacterial community of white shrimp Penaeus vannamei fed with green seaweeds

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Abstract

In recent years, development of sustainable and ecological food production has gained worldwide interest. It seems clear that this phenomenon is causing changes in aquaculture-focused research, with the development of new integration systems. However, it is still necessary to understand different aspects involved in integrated systems, including co-culture systems such as shrimp and seaweed. This study evaluated the effect of green seaweeds as food source on white shrimp Penaeus vannamei intestinal bacterial communities. Shrimp were evaluated after a 4-week experimental trial under different diet treatments: fed with only pellet (P), only Ulva clathrata (UC), U. clathrata + pellet (UCP), only Ulva lactuca (UL), and U. lactuca + pellet (ULP). In terms of growth and survival, no significant differences (P > 0.05) were found between ULP and UCP treatments compared with the control (P). Analysis of the bacterial biota of shrimp intestine revealed significant differences on community composition in ULP, UL, and UC compared with the control (P) (P < 0.05). We found that Proteobacteria is the most abundant phylum in all treatments, followed by Bacteroidetes for UC, UCP, and UL and Actinobacteria for P and ULP treatments. Shrimp fed only with seaweed U. lactuca (UL, ULP) had a significantly higher abundance of Rubritalea, Lysinibacillus, Acinetobacter, and Blastopirellula, and for U. clathrata treatments (UC, UCP), it was Litoreibacter. Relative abundance of Vibrio was higher in the control (P), showing a decrease in UC and UL treatments. Our findings provide a better understanding of integrated aquaculture systems, specifically those utilizing seaweed as natural feed source.

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Acknowledgments

We are thankful to Gran Mahr laboratory, especially Samuel Echeverria and Omar Camorlinga for kindly donating the shrimp juveniles; Armando León from Algal Tech SAPI de CV for providing U. clathrata seaweed; Sandra de la Paz-Reyes from Laboratory of Aquaculture Nutrition, Pablo Monsalvo-Spencer, and Gabriel Robles Villegas from the laboratory for acclimatization and maintenance of aquatic organisms; and Gabriela Mendoza-Carrión from Genomics and Bioinformatics Laboratory at CIBNOR for all the facilities and technical support during the experiment development. This work was performed within the framework of a UBO/CIBNOR agreement to receive Master’s degree candidates from UBO to complete their MS thesis project.

Data availability statement

Data available from corresponding author on request.

Funding

This work was supported by a grant to Maxence Gemin by the “Laboratoire d’Excellence” LabexMER (ANR-10-LABX-19) and co-funded by a grant from the French government under the program “Investissements d’Avenir”.

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Authors and Affiliations

  1. CONACYT, Centro de Investigaciones Biológicas del Noroeste, S.C., Calle IPN 195, 23096, La Paz, B.C.S., Mexico

    Regina Elizondo-González, Eduardo Quiroz-Guzmán & Alberto Peña-Rodríguez

  2. Department of Agricultural and Biosystems Engineering, Iowa State University, Ames, IA, 50011, USA

    Adina Howe, Fan Yang & Jared Flater

  3. Institut Universitaire Européen de la Mer (IUEM), Université de Bretagne Occidentale (UBO), 29280, Plouzané, France

    Maxence Gemin

  4. Centro de Investigaciones Biológicas del Noroeste, S.C., Calle IPN 195, 23096, La Paz, B.C.S., Mexico

    Maxence Gemin & Elena Palacios

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  1. Regina Elizondo-González
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  2. Eduardo Quiroz-Guzmán
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  3. Adina Howe
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  4. Fan Yang
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  8. Alberto Peña-Rodríguez
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Correspondence to Alberto Peña-Rodríguez.

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Online Resources 1

Box plot of richness and bacterial diversity by individual replicates. Observed OTUs, Chao1, Shannon and InvSimpson indices were estimated for treatments with only pellet (P), only U. clathrata (UC), U. clathrata + pellet (UCP), only U. lactuca (UL) and U. lactuca + pellet (ULP) (PNG 70 kb)

Online Resources 2

Relative read abundance of different bacterial phyla by individual replicate for each treatment (only pellet (P), only U. clathrata (UC), U. clathrata + pellet (UCP), only U. lactuca (UL) and U. lactuca + pellet (ULP) (PNG 81 kb)

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Elizondo-González, R., Quiroz-Guzmán, E., Howe, A. et al. Changes on the intestinal bacterial community of white shrimp Penaeus vannamei fed with green seaweeds. J Appl Phycol 32, 2061–2070 (2020). https://doi.org/10.1007/s10811-020-02072-w

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