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Algicidal potential of cultivable bacteria from pelagic waters against the toxic dinoflagellate Pyrodinium bahamense (Dinophyceae)

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Abstract

Associated and algicidal bacteria play roles in the succession and decline of phytoplankton blooms, including those of harmful algal bloom (HAB)-forming species. Limited studies on HAB-associated bacterial ecology have resulted in our incomplete understanding of HABs dynamics. Diverse phytoplankton-bacterial interactions have also led to studies on their potential as biocontrol tools for HABs mitigation. Here, we tested 48 cultivable pelagic bacteria from three HAB-affected areas in the Philippines (Bolinao, Sorsogon, and Matarinao) against non-axenic cultures of the toxic, thecate dinoflagellate Pyrodinium bahamense. Co-incubation with live cultures of these isolates exhibited varying levels of algicidal activities suggesting that it may not be a phylogenetically conserved property. Furthermore, majority of the isolates elicited activity against the thecate test species P. bahamense, which was not present in the area where the bacteria were isolated, implying non-specificity of action. Exposure to bacterial cells resulted in Pyrodinium pellicle cyst formation. Despite this, however, cell lysis and decline in total cell abundance were still observed, indicating strong algicidal potency of the isolates. In depth understanding of the interplay between environmental factors and algicidal bacteria-microalgal interactions may provide significant insights on the management of HABs.

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Acknowledgments

This study is part of the research projects: Ecology and Oceanography of Harmful Algal Blooms in the Philippines (PhilHABs) Project 2. Microbial Community (HAB Species and Associated Bacteria) Composition and Succession and HAB Genomics Project 1. Molecular Toxins and Other National Products from HAB Causative Organisms and Bacteria for Standards and Tools. We would also like to thank Dr. Windell L. Rivera, Dr. Lilibeth Salvador-Reyes and Dr. Mary Ann G. Santos for their constructive comments, Sammy Santos and Estrelita Flores for their help in maintaining the algal cultures, and Jenelyn Mendoza for some technical assistance.

Funding

PhilHABs Project 2 and HAB Genomics Project 1 were  funded and supported by the Department of Science Technology (DOST) through the Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (PCAARRD). Additional support was given by an MSI in-house grant to DFLO for the CLSM work.

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Authors and Affiliations

  1. The Marine Science Institute, College of Science, University of the Philippines, Diliman, 1101, Quezon City, Philippines

    Jenelle Clarisse R. Dungca-Santos, Frenchly Joyce O. Caspe, Francis A. Tablizo, Daniel John E. Purganan, Rhodora V. Azanza & Deo Florence L. Onda

  2. Philippine Genome Center, University of the Philippines, Diliman, 1101, Quezon City, Philippines

    Francis A. Tablizo

  3. Department of Science and Technology, National Academy of Science and Technology, 3/F, Science Heritage Building, DOST Complex, General Santos Ave, 4044, Taguig City, Philippines

    Rhodora V. Azanza

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  1. Jenelle Clarisse R. Dungca-Santos
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  2. Frenchly Joyce O. Caspe
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  3. Francis A. Tablizo
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  4. Daniel John E. Purganan
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  5. Rhodora V. Azanza
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  6. Deo Florence L. Onda
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Correspondence to Deo Florence L. Onda.

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Dungca-Santos, J.C.R., Caspe, F.J.O., Tablizo, F.A. et al. Algicidal potential of cultivable bacteria from pelagic waters against the toxic dinoflagellate Pyrodinium bahamense (Dinophyceae). J Appl Phycol 31, 3721–3735 (2019). https://doi.org/10.1007/s10811-019-01839-0

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  • DOI: https://doi.org/10.1007/s10811-019-01839-0

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