Skip to main content
Log in

Mutants of Scenedesmus sp. for purifying highly concentrated cellulosic ethanol wastewater and producing biomass simultaneously

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

To purify cellulosic ethanol wastewater (CEW) and obtain algae simultaneously, culturing microalgae using CEW as the medium is a potentially promising technology. In this study, mutation breeding of Scenedesmus sp. induced by ultraviolet (UV) and ethyl methanesulfonate (EMS) was performed, and three mutant strains were screened using undiluted CEW. Among them, the mutant MU1 showed a high biomass yield of 1075 mg L−1 and biomass productivity of 39.81 mg L−1 day−1, and the lipid and protein content of MU1 can reach up to 21.4 and 41.9% (dry weight of biomass), respectively. Meanwhile, the chemical oxygen demand (COD), total ammonia nitrogen (TAN), total phosphorus (TP) and chroma (CHR) removal percentages in CEW reached to 33.5, 71.2, 46.4 and 17.8%, respectively. These results indicated that MU1 is suitable for purifying CEW and producing biomass simultaneously, providing a prospective way for CEW treatment and low-cost microalgae cultivation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Amini H, Wang L, Shahbazi A (2016) Effects of harvesting cell density, medium depth and environmental factors on biomass and lipid productivities of Chlorella vulgaris grown in swine wastewater. Chem Eng Sci 152:403–412

    Article  CAS  Google Scholar 

  • Baer S, Heining M, Schwerna P, Buchholz R, Hübner H (2016) Optimization of spectral light quality for growth and product formation in different microalgae using a continuous photobioreactor. Algal Res 14:109–115

    Article  Google Scholar 

  • Cabanelas ITD, Ruiz J, Arbib Z, Chinalia FA, Garrido-Pérez C, Rogalla F, Nascimento IA, Perales JA (2013) Comparing the use of different domestic wastewaters for coupling microalgal production and nutrient removal. Bioresour Technol 131:429–436

    Article  CAS  PubMed  Google Scholar 

  • Chiu S, Kao C, Chen T, Chang Y, Kuo C, Lin C (2015) Cultivation of microalgal Chlorella for biomass and lipid production using wastewater as nutrient resource. Bioresour Technol 184:179–189

    Article  CAS  PubMed  Google Scholar 

  • de Mooij T, de Vries G, Latsos C, Wijffels RH, Janssen M (2016) Impact of light color on photobioreactor productivity. Algal Res 15:32–42

    Article  Google Scholar 

  • Di Pippo F, Ellwood NTW, Guzzon A, Siliato L, Micheletti E, De Philippis R, Albertano PB (2012) Effect of light and temperature on biomass, photosynthesis and capsular polysaccharides in cultured phototrophic biofilms. J Appl Phycol 24:211–220

    Article  CAS  Google Scholar 

  • Duong VT, Ahmed F, Thomas-Hall SR, Quigley S, Nowak E, Schenk PM (2015) High protein- and high lipid-producing microalgae from northern Australia as potential feedstock for animal feed and biodiesel. Front Bioeng Biotech 3:53

    Article  Google Scholar 

  • Dwivedi P, Alavalapati JRR, Lal P (2009) Cellulosic ethanol production in the United States: conversion technologies, current production status, economics, and emerging developments. Energy Sust Dev 13:174–182

    Article  CAS  Google Scholar 

  • Fu F, Sui Z, Zhou W, Wang J, Chang L, Ci S (2014) UV-irradiation mutation of tetraspores of Gracilariopsis lemaneiformis and screening of thermotolerant strains. J Appl Phycol 26:647–656

    Article  CAS  Google Scholar 

  • Garcia-Moscoso JL, Obeid W, Kumar S, Hatcher PG (2013) Flash hydrolysis of microalgae (Scenedesmus sp.) for protein extraction and production of biofuels intermediates. J Supercrit Fluid 82:183–190

    Article  CAS  Google Scholar 

  • Gour RS, Chawla A, Singh H, Chauhan RS, Kant A (2016) Characterization and screening of native Scenedesmus sp. isolates suitable for biofuel feedstock. PLoS One 11(5):e0155321

    Article  PubMed  PubMed Central  Google Scholar 

  • Guerrero-Cabrera L, Rueda JA, García-Lozano H, Navarro AK (2014) Cultivation of Monoraphidium sp., Chlorella sp. and Scenedesmus sp. algae in batch culture using Nile tilapia effluent. Bioresour Technol 161:455–460

    Article  CAS  PubMed  Google Scholar 

  • Han S, Jin W, Tu R, Abomohra AE, Wang Z (2016) Optimization of aeration for biodiesel production by Scenedesmus obliquus grown in municipal wastewater. Bioprocess Biosyst Eng 39:1073–1079

    Article  CAS  PubMed  Google Scholar 

  • Hintz HF, Heitman H, Weir WC, Torrell DT, Myers JH (1966) Nutritive value of algae grown on sewage. J Anim Sci 25:675–681

    Article  Google Scholar 

  • Huo S, Wang Z, Zhu S, Zhou W, Dong R, Yuan Z (2012) Cultivation of Chlorella zofingiensis in bench-scale outdoor ponds by regulation of pH using dairy wastewater in winter, South China. Bioresour Technol 121:76–82

    Article  CAS  PubMed  Google Scholar 

  • Jia Q, Xiang W, Yang F, Hu Q, Tang M, Chen C, Wang G, Dai S, Wu H, Wu H (2016) Low-cost cultivation of Scenedesmus sp. with filtered anaerobically digested piggery wastewater: biofuel production and pollutant remediation. J Appl Phycol 28:727–736

    Article  CAS  Google Scholar 

  • Knothe G (2008) “Designer” biodiesel: optimizing fatty ester composition to improve fuel properties. Energ Fuel 22:1358–1364

    Article  CAS  Google Scholar 

  • Lavoie A, de la Noüe J (1985) Hyperconcentrated culture of Scenedesmus obliquus: a new approach for wastewater tertiary treatment? Water Res 19:1437–1442

    Article  CAS  Google Scholar 

  • Leite GB, Paranjape K, Hallenbeck PC (2016) Breakfast of champions: fast lipid accumulation by cultures of Chlorella and Scenedesmus induced by xylose. Algal Res 16:338–348

    Article  Google Scholar 

  • Li Y, Chen YF, Chen P, Min M, Zhou W, Martinez B, Zhu J, Ruan R (2011) Characterization of a microalga Chlorella sp. well adapted to highly concentrated municipal wastewater for nutrient removal and biodiesel production. Bioresour Technol 102:5138–5144

    Article  CAS  PubMed  Google Scholar 

  • Liu S, Zhao Y, Liu L, Ao X, Ma L, Wu M, Ma F (2015) Improving cell growth and lipid accumulation in green microalgae Chlorella sp. via UV irradiation. Appl Biochem Biotech 175:3507–3518

    Article  CAS  Google Scholar 

  • Luo L, He H, C2 Y, Wen S, Zeng G, Wu M, Zhou Z, Lou W (2016) Nutrient removal and lipid production by Coelastrella sp. in anaerobically and aerobically treated swine wastewater. Bioresour Technol 216:135–141

  • Mabee WE, McFarlane PN, Saddler JN (2011) Biomass availability for lignocellulosic ethanol production. Biomass Bioenergy 35:4519–4529

    Article  Google Scholar 

  • Rasdi NW, Qin JG (2015) Effect of N:P ratio on growth and chemical composition of Nannochloropsis oculata and Tisochrysis lutea. J Appl Phycol 27:2221–2230

    Article  CAS  Google Scholar 

  • Ryckebosch E, Muylaert K, Foubert I (2012) Optimization of an analytical procedure for extraction of lipids from microalgae. J Am Oil Chem Soc 89:189–198

    Article  CAS  Google Scholar 

  • Shan L, Liu J, Yu Y, Ambuchi JJ, Feng Y (2016) Characterization of persistent colors and decolorization of effluent from biologically treated cellulosic ethanol production wastewater. Environ Sci Pollut Res 23:10215–10222

    Article  CAS  Google Scholar 

  • Slade R, Bauen A (2013) Micro-algae cultivation for biofuels: cost, energy balance, environmental impacts and future prospects. Biomass Bioenergy 53:29–38

    Article  Google Scholar 

  • Wang H, Pampati N, McCormick WM, Bhattacharyya L (2016) Protein nitrogen determination by Kjeldahl digestion and ion chromatography. J Pharm Sci 105:1851–1857

    Article  CAS  PubMed  Google Scholar 

  • Wen S, Liu H, He H, Luo L, Li X, Zeng G, Zhou Z, Lou W, Yang C (2016) Treatment of anaerobically digested swine wastewater by Rhodobacter blasticus and Rhodobacter capsulatus. Bioresour Technol 222:33–38

    Article  CAS  PubMed  Google Scholar 

  • Wu HL, Hu ZB, Chai XS, Wang C, Yang OM (2016) Rapid detection of ammonia nitrogen in water with dual-wavelength spectroscopy. Spectrosc Spectr Anal 36:1396–1399

    CAS  Google Scholar 

  • Wu X, Tong Y, Shankar K, Baumgardner JN, Kang J, Badeaux J, Badger TM, Ronis MJ (2011) Lipid fatty acid profile analyses in liver and serum in rats with nonalcoholic steatohepatitis using improved gas chromatography−mass spectrometry methodology. J Agric Food Chem 59:747–754

    Article  CAS  PubMed  Google Scholar 

  • Xu X, Shen Y, Chen J (2015) Cultivation of Scenedesmus dimorphus for C/N/P removal and lipid production. Electron J Biotechnol 18:46–50

    Article  CAS  Google Scholar 

  • Yang L, Tan X, Li D, Chu H, Zhou X, Zhang Y, Yu H (2015) Nutrients removal and lipids production by Chlorella pyrenoidosa cultivation using anaerobic digested starch wastewater and alcohol wastewater. Bioresour Technol 181:54–61

    Article  CAS  PubMed  Google Scholar 

  • Zhan J, Zhang Q, Qin M, Hong Y (2016) Selection and characterization of eight freshwater green algae strains for synchronous water purification and lipid production. Front Env Sci Eng 10:548–558

    Article  CAS  Google Scholar 

  • Zhang Y, He M, Zou S, Fei C, Yan Y, Zheng S, Rajper AA, Wang C (2016) Breeding of high biomass and lipid producing Desmodesmus sp. by ethylmethane sulfonate-induced mutation. Bioresour Technol 207:268–275

    Article  CAS  PubMed  Google Scholar 

  • Zhao YB (2013) Study on treatment of cellulose fuel ethanol wastewater and application. Adv Mater Res 777:365–369

    Article  Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the financial support from the Natural Science Foundation of Henan Province of China (162300410247); the Foundation and Frontier Technology Research Project of Henan Province of China (162300410007), the Sino-Thai project of National Natural Science Foundation of China (51561145015), and the Key project of Natural Science Foundation of Guangdong Province (No. 2015A030311022).

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to Chun Chang or Jing Bai.

Electronic supplementary material

ESM 1

(PDF 258 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, Q., Chang, C., Bai, J. et al. Mutants of Scenedesmus sp. for purifying highly concentrated cellulosic ethanol wastewater and producing biomass simultaneously. J Appl Phycol 30, 969–978 (2018). https://doi.org/10.1007/s10811-017-1311-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-017-1311-3

Keywords

Navigation