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Gold (III) bioreduction by cyanobacteria with special reference to in vitro biosafety assay of gold nanoparticles

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Abstract

Bioreduction of Au3+ to Au0 and subsequent synthesis of gold nanoparticles using three cyanobacterial strains Leptolyngbya tenuis, Coleofasciculus chthonoplastes, and Nostoc ellipsosporum was investigated. The optimized condition for maximum gold nanoparticle synthesis was determined as 20 mg cyanobacterial biomass per 100 mL of 15 mg L−1 Au3+ solution as inoculum size at pH 5. At the onset of nanoparticle synthesis (up to 3 h) increased activity of catalase, ascorbate peroxidase, super oxide dismutase, and malondialdehyde content along with rapid loss of pigments and protein content (1–72 h) indicated gold toxicity at cellular level. Associated changes in thallus morphology were also significant. Nostoc biomass produced spherical- to irregular-shaped nanoparticles with diverse sizes and small number of nanorods. On the other hand, Coleofasciculus showed nanoparticle synthesis at extracellular medium which was either absent in Nostoc or very low in Leptolyngbya. Biosafety analysis by MTT assay using peripheral blood mononuclear cells (PBMC), two cancer cell lines viz. T cell acute (T-ALL) and human acute lymphoblastic leukemia (MOLT-4) and antibacterial effect against Pseudomonas aeruginosa and Staphylococcus aureus showed no toxic effect of synthesized gold nanoparticles.

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Acknowledgments

We would like to acknowledge the financial assistance of University Grants Commission (UGC-RFSMS). We sincerely acknowledge Prof. Anjan Kumar Dasgupta of the Department of Biochemistry, University of Calcutta, for his cooperation during the work. We acknowledge Dr. Sagarmoy Ghosh, Department of Microbiology, C.U., for his help and support. We are also thankful to Ms. Urmila Goswami for her help during the HRTEM studies and Mr. Tridib Das for the ESEM studies.

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Authors and Affiliations

  1. Phycology Laboratory, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, West Bengal, 700019, India

    Dipannita Parial & Ruma Pal

  2. Laboratory of Cell and Molecular Biology, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Kolkata, 700019, India

    Priya K. Gopal & Santanu Paul

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  1. Dipannita Parial
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Correspondence to Ruma Pal.

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Parial, D., Gopal, P.K., Paul, S. et al. Gold (III) bioreduction by cyanobacteria with special reference to in vitro biosafety assay of gold nanoparticles. J Appl Phycol 28, 3395–3406 (2016). https://doi.org/10.1007/s10811-016-0880-x

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