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Toxic effect on the membrane system and cell proliferation of Prorocentrum donghaiense caused by the novel algicidal fungus Talaromyces purpurogenus YL13

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Abstract

A fungal strain YL13 with algicidal activity against the dinoflagellate Prorocentrum donghaiense was isolated from Wild Fox Island, Zhuhai, China, and identified as Talaromyces purpurogenus YL13 on the basis of 18S ribosomal RNA (rRNA) sequence. Strain YL13 exhibited algicidal activity through the mechanism of indirect attack, and its algicidal activity was improved from 80.3 to 96.8 % by optimization of environmental and nutrient factors with response surface methodology (RSM). Effects of strain YL13 on the membrane system and cell proliferating of P. donghaiense were investigated to elucidate the algicidal mechanism. The increase in both ATPase activities and malondialdehyde (MDA) contents suggested that the membrane in algal cells was damaged. Damage was observed in atomic force microscopy (AFM) images and the surface morphology of cells. Real-time PCR assay showed changes in the transcript abundance of proliferating cell nuclear antigen (PCNA) gene. The release of nucleic acids and changes of PCNA gene expression indicated that DNA synthesis was affected, and this influenced cell proliferation and the membrane system of P. donghaiense. The fungal supernatant might be potentially used as a bioagent for controlling harmful algae. Strain YL13 is the first record of a T. purpurogenus being algicidal to the harmful dinoflagellate P. donghaiense, and this is the first report to explore the mechanism of toxic effects on membrane system and cell proliferation of the marine T. purpurogenus against harmful P. donghaiense.

The toxicity mechanism of algicidal fungus Talaromyces purpurogenus YL13 against Prorocentrum donghaiense

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Acknowledgments

This work was financially supported by the Joint Fund of National Natural Science Foundation of China-Guangdong (Project No. U11330003) and the National Natural Science Foundation of China (Project No. 41076068) and Science and Technology Program of Guangdong, China (Project No. 2014A020217007).

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Authors and Affiliations

  1. Key Laboratory of Water and Soil Pollution Control and Bioremediation of Guangdong Higher Education Institutes, School of Environment, Jinan University, Guangzhou, 510632, China

    Wanjiao Shu, Ling Zhao & Shaoling Hou

  2. Guangzhou Key Laboratory of Environmental Exposure and Health, School of Environment, Jinan University, No. 601, Huangpu Da Dao Xi, Guangzhou, 510632, China

    Wanjiao Shu, Ling Zhao & Shaoling Hou

  3. Griffith School of Engineering, Griffith University, Brisbane, Queensland, 4111, Australia

    Qiming Jimmy Yu

  4. Department of Chemistry, Jinan University, No. 601, Huangpu Da Dao Xi, Guangzhou, 510632, China

    Shuo Tan & Pinghe Yin

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  1. Wanjiao Shu
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  2. Ling Zhao
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  3. Shaoling Hou
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  4. Qiming Jimmy Yu
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  5. Shuo Tan
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  6. Pinghe Yin
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Correspondence to Ling Zhao or Pinghe Yin.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Esm 1

Growth curve of Strain YL13 and the algicidal percentage treated by each growth phase of strain YL13 supernatant. All error bars indicate SE of three replicates. (PDF 67 kb)

Esm 2

The effect of different culture temperature on the fungal growth rate and algicidal percentage. All error bars indicate SE of three replicates. (PDF 66 kb)

Esm 3

The effect of different culture pH on the fungal growth rate and algicidal percentage. All error bars indicate SE of three replicates. (PDF 52 kb)

Esm 4

The effect of different culture salinity on the fungal growth rate and algicidal percentage. All error bars indicate SE of three replicates. (PDF 54 kb)

Esm 5

The effect of different culture rotation speed on the fungal growth rate and algicidal percentage. All error bars indicate SE of three replicates. (PDF 64 kb)

Esm 6

The effect of different carbon sources on the fungal growth rate and algicidal percentage. All error bars indicate SE of three replicates. (PDF 55 kb)

Esm 7

The effect of different nitrogen sources on the fungal growth rate and algicidal percentage. All error bars indicate SE of three replicates. (PDF 64 kb)

Esm 8

The effect of different phosphorus sources on the fungal growth rate and algicidal percentage. All error bars indicate SE of three replicates. (PDF 54 kb)

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Shu, W., Zhao, L., Hou, S. et al. Toxic effect on the membrane system and cell proliferation of Prorocentrum donghaiense caused by the novel algicidal fungus Talaromyces purpurogenus YL13. J Appl Phycol 29, 275–284 (2017). https://doi.org/10.1007/s10811-016-0878-4

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  • DOI: https://doi.org/10.1007/s10811-016-0878-4

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