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The effect of Ascophyllum nodosum (Ochrophyta) extract powder on the epibiosis of Kappaphycus alvarezii (Rhodophyta) commercially cultivated on floating rafts

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Abstract

Acadian marine plant extract powder (AMPEP), a product derived from Ascophyllum nodosum, has been widely used as a plant growth stimulant and recently has been tested on seaweeds with successful results on increasing growth and preventing epibiosis in Kappaphycus alvarezii cultivation. Although, the AMPEP ability to deal with epibiosis on seedlings cultivated in tubular nets and its commercial implications are still unknown. Between July 2012 and September 2013, three sampling periods were randomly selected at a Brazilian commercial farm in order to evaluate the effect of AMPEP on K. alvarezii daily growth rate, carrageenan yield and quality, and epibiont settling. Ten tubular nets containing three 100 g seedlings each (five treated with AMPEP and five not) were tied on floating rafts. Control samples displayed higher biomass of epibionts [0.76 ± 0.52 g (dry mass) tubular net−1] when compared to the AMPEP-treated samples [0.26 ± 0.27 g (dry mass) tubular net−1]. Carrageenan yield (44.65 ± 7.09 %) showed no significant difference between treatments (t = 0.46; p = 0.64), with larger percentages by the end of the third sampling period (F = 130.96, p < 0.001). When comparing AMPEP treatment with the control, carrageenan gel strength (252.14 ± 82.4 g cm−3) and viscosity (106.72 ± 45.11 cP) showed opposite results. The gel strength of AMPEP-treated samples was higher than their controls (t = 2.88; p = 0.007), while the gel viscosity of samples treated with AMPEP was lower than samples from their control (t = −2.4; p = 0.02). K. alvarezii daily growth rates between treatments were not different (t = 0.92; p = 0.36), exhibiting low commercial values probably due to herbivory. It was concluded that AMPEP was efficient against epibiont settlement on K. alvarezii cultivated on tubular nets tied on floating rafts maintaining favorable commercial results for carrageenan yield.

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References

  • Borlongan IAG, Tibudos KR, Yunque DAT, Hurtado AQ, Critchley AT (2011) Impact of AMPEP on the growth and occurrence of epiphytic Neosiphonia infestation on two varieties of commercially cultivated Kappaphycus alvarezii grown at different depths in the Philippines. J Appl Phycol 23:615–621

    Article  Google Scholar 

  • Craigie JS (2011) Seaweed extract stimuli in plant science and agriculture. J Appl Phycol 23:371–393

    Article  CAS  Google Scholar 

  • Creed JC, Ramos RA, Casares FA, Oliveira AES (2007) Características ambientais: substrato da orla costeira. In: Creed JC, Pires DO, Figueredo MAO (eds) Biodiversidade Marinha da Baía da Ilha Grande. Ministério do MeioAmbiente, Brasília, pp 133–151

    Google Scholar 

  • FAO (2014) The state of world fisheries and aquaculture 2014. FAO, Rome, 223p

    Google Scholar 

  • Góes HG, Reis RP (2011) An initial comparison of tubular netting versus tie–tie methods of cultivation for Kappaphycus alvarezii (Rhodophyta, Solieriaceae) on the south coast of Rio de Janeiro State, Brazil. J Appl Phycol 23:607–613

    Article  Google Scholar 

  • Góes HG, Reis RP (2012) Temporal variation of the growth, carrageenan yield and quality of Kappaphycus alvarezii (Rhodophyta, Gigartinales) cultivated at Sepetiba Bay, southeastern Brazilian coast. J Appl Phycol 24:173–180

    Article  Google Scholar 

  • Hayashi L, Paula EJ, Chow F (2007) Growth rate and carrageenan analyses in four strains of Kappaphycus alvarezii (Rhodophyta, Gigartinales) farmed in the subtropical waters of São Paulo State, Brazil. J Appl Phycol 19:393–399

    Article  CAS  Google Scholar 

  • Hayashi L, Santos AA, Faria GSM, Nunes BG, Souza MS, Fonseca ALD, Barreto PLM, Oliveira EC, Bouzon ZL (2011) Kappaphycus alvarezii (Rhodophyta, Areschougiaceae) cultivated in subtropical waters in Southern Brazil. J Appl Phycol 23:337–343

    Article  Google Scholar 

  • Hurtado AQ, Joe M, Sanares RC, Fan D, Prithiviraj B, Critchley AT (2012) Investigation of the application of Acadian Marine Plant Extract Powder (AMPEP) to enhance the growth, phenolic content, free radical scavenging, and iron chelating activities of Kappaphycus Doty (Solieriaceae, Gigartinales, Rhodophyta). J Appl Phycol 24:601–611

    Article  CAS  Google Scholar 

  • Hurtado AQ, Reis RP, Loureiro RR, Crichley AT (2014) Kappaphycus (Rhodophyta) cultivation: problems and impacts of Acadian Marine Plant Extract Powder. In: Pereira L, Neto JM (eds) Marine algae biodiversity, taxonomy, environmental assessment, and biotechnology. CRC Press, Boca Raton, pp 251–299

    Google Scholar 

  • Jayaraman J, Norrie J, Punja ZK (2011) Commercial extract from the brown seaweed Ascophyllum nodosum reduces diseases in green house cucumber. J Appl Phycol 23:353–361

    Article  Google Scholar 

  • Khan W, Rayirath UP, Subramanian UP, Jitesh MN, Rayorath P, Hodges DM, Critchley DM, Craigie JS, Norrie J, Prithiviraj B (2009) Seaweed extracts as biostimulant of plant growth and development. J Plant Growth Regul 28:386–399

    Article  CAS  Google Scholar 

  • Krug PJ (2006) Defense of benthic invertebrates against surface colonization by larvae: a chemical arms race. In: Fusetani N, Claire AS (eds) Molecular and subcellular biology subseries marine molecular biotechnology: antifouling compounds. Springer, Berlin, pp 1–54

    Google Scholar 

  • Loureiro RR, Reis RP, Critchley AT (2010) In vitro cultivation of three Kappaphycus alvarezii (Rhodophyta, Areschougiaceae) variants (green, red and brown) exposed to a commercial extract of the brown alga Ascophyllum nodosum (Fucaceae, Ochrophyta). J Appl Phycol 22:101–104

    Article  Google Scholar 

  • Loureiro RR, Reis RP, Berrogain FD, Critchley AT (2012) Extract powder from the brown alga Ascophyllum nodosum (Linnaeus) Le Jolis (AMPEP): a vaccine-like effect on Kappaphycus alvarezii (Doty) Doty ex P.C. Silva. J Appl Phycol 24:427–432

    Article  Google Scholar 

  • Loureiro RR, Reis RP, Berrogain FD, Critchley AT (2014a) Effects of a commercial extract of the brown alga Ascophyllum nodosum on the biomass production of Kappaphycus alvarezii (Doty) Doty ex P.C. Silva and its carrageenan yield and gel quality cultivated in Brazil. J Appl Phycol 24:427–432

    Article  Google Scholar 

  • Loureiro RR, Reis R, Marroig R (2014b) Effect of the commercial extract of the brown alga Ascophyllum nodosum Mont. on Kappaphycus alvarezii (Doty) Doty ex P.C. Silva in situ submitted to lethal temperatures. J Appl Phycol 26:629–634

    Article  Google Scholar 

  • Marroig R, Reis RP (2011) Does biofouling influence Kappaphycus alvarezii (Doty) Doty ex Silva farming production in Brazil? J Appl Phycol 23:925–931

    Article  Google Scholar 

  • Mendoza WG, Ganzon-Fortes ET, Villanueva RD, Romero JB, Montaño MNE (2006) Tissue age as a factor affecting carrageenan quantity and quality in farmed Kappaphycus striatum (Schmitz) Doty ex Silva. Bot Mar 49:57–64

    Article  CAS  Google Scholar 

  • Nylund GM, Pavia H (2005) Chemical versus mechanical inhibition of fouling in the red alga Dilsea carnosa. Mar Ecol Prog Ser 299:111–121

    Article  Google Scholar 

  • Pereira L, Critchley AT, Amado AM, Ribeiro-Claro PJA (2009) A comparative analysis of phycocolloids produced by underutilized versus industrially utilized carragenophytes (Gigartinales, Rhodophyta). J Appl Phycol 21:599–605

    Article  CAS  Google Scholar 

  • Reis RP, Yoneshigue-Valentin Y, Santos CP (2008) Spatial and temporal variation of Hypnea musciformis carrageenan (Rhodophyta - Gigartinales) from natural beds in Rio de Janeiro State, Brazil. J Appl Phycol 20:1–8

    Article  Google Scholar 

  • Reis RP, Pereira RRC, Góes HG (2015) The efficiency of tubular netting method of cultivation for Kappaphycus alvarezii (Rhodophyta, Gigartinales) on the southeastern Brazilian coast. J Appl Phycol 27:421–426

    Article  CAS  Google Scholar 

  • Sharma HSS, Fleming C, Selby C, Rao JR, Martin T (2014) Plant biostimulants: a review on the processing of macroalgae and use of extracts for crop management to reduce abiotic and biotic stresses. J Appl Phycol 26:465–490

    Article  CAS  Google Scholar 

  • Terada R, Vo TD, Nishihara G, Shioya K, Shimada S, Kawagushi S (2015) The effect of irradiance and temperature on the photosynthesis and growth of a cultivated red alga Kappaphycus alvarezii (Solieriaceae) from Vietnam, based on in situ and in vitro measurements. J Appl Phycol 27:1–11. doi:10.1007/s10811-015-0557-x

    Article  Google Scholar 

  • Vairappan CS, Chung CS, Matsunaga S (2014) Effect of epiphyte infection on physical and chemical properties of carrageenan produced by Kappaphycus alvarezii Doty (Soliericeae, Gigartinales, Rhodophyta). J Appl Phycol 26:923–931

    Article  Google Scholar 

  • Villanueva RD, Romero JB, Montaño MNE, de la Peña PO (2011) Harvest optimization of four Kappaphycus species from the Philippines. Biomass Bioenergy 35:1311–1316

    Article  CAS  Google Scholar 

  • Yong YS, Yong WTL, Anton A (2013) Analysis of formulae for determination of seaweed growth rate. J Appl Phycol 25:1831–1834

    Article  Google Scholar 

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Acknowledgments

This research was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação de Pessoal de Aperfeiçoamento de Nível Superior (CAPES). The authors would like to thank Henrique Geromél de Góes for providing access to the K. alvarezii farm and his assistance on the experiments in the field and to Dr. Beatriz Castelar for the abiotic data readings.

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Authors and Affiliations

  1. Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rua Pacheco Leão, 915, Rio de Janeiro, RJ, CEP 22460-030, Brazil

    Rafael Guedes Marroig & Renata Perpetuo Reis

  2. Biology Department, Ave Maria University, 5050 Ave Maria Blvd, Ave Maria, FL, 34142, USA

    Rafael Rodrigues Loureiro

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  1. Rafael Guedes Marroig
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  2. Rafael Rodrigues Loureiro
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  3. Renata Perpetuo Reis
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Correspondence to Renata Perpetuo Reis.

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Guedes Marroig, R., Rodrigues Loureiro, R. & Perpetuo Reis, R. The effect of Ascophyllum nodosum (Ochrophyta) extract powder on the epibiosis of Kappaphycus alvarezii (Rhodophyta) commercially cultivated on floating rafts. J Appl Phycol 28, 2471–2477 (2016). https://doi.org/10.1007/s10811-015-0770-7

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  • DOI: https://doi.org/10.1007/s10811-015-0770-7

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