Abstract
Effects of two intensities (1 and 5 W m−2) of UV-B radiation on the synthesis of UV-absorbing compounds in a terrestrial cyanobacterium Nostoc flagelliforme were investigated. UV-B radiation resulted in lower biomass. Short period (less than 12 h) of UV-B radiation caused an increase of chlorophyll a content, but subsequent duration of treatment (more than 24 h) resulted in a rapid decrease. N. flagelliforme synthesized UV-absorbing compounds such as scytonemin and mycosporine-like amino acids (MAAs) in response to UV-B radiation. Upon 48 h of exposure to UV-B radiation, scytonemin content in cells increased by 103.8 and 164.0 % at 1 and 5 W m−2, respectively. Oligosaccharide-linked mycosporine-like amino acids increased by 145.5 % after 12 h at 5 W m−2 and 114.5 % after 48 h at 1 W m−2 UV-B radiation. HPLC analysis showed that nine MAAs existed in N. flagelliforme cells both from liquid suspension culture and field colony. But the concentration and kinds of them were different. At the two distinct levels of UV-B radiation, the content of particular MAAs increased, declined, or remained unchanged. Moreover, the appearance of two new MAAs was observed.
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Belnap J, Phillips SL, Miller ME (2004) Response of desert biological soil crusts to alterations in precipitation frequency. Oecologia 141:306–316
Böhm GA, Pfleiderer W, Böger P, Scherer S (1995) Structure of a novel oligosaccharide-mycosporine-amino acid ultraviolet A/B sunscreen pigment from the terrestrial cyanobacterium Nostoc commune. J Biol Chem 270:8536–8539
Bornman JF (1989) New trends in photobiology: target sites of UV-B radiation in photosynthesis of higher plants. J Photochem Photobiol B: Biol 4:145–158
Bowker MA, Reed SC, Belnap J, Phillips SL (2002) Temporal variation in community composition, pigmentation, and Fv/Fm of desert cyanobacterial soil crusts. Microb Ecol 43:13–25
Budel B (2003) Biological soil crusts of Asia including the Don and Volga Region. In: Belnap J, Lange OL (eds) Biological soil crusts: structure, function, and management. Springer, Berlin, pp 87–89
Chen X, Jia S, Wang Y, Wang N (2011) Biological crust of Nostoc flagelliforme (cyanobacteria) on sand bed materials. J Appl Phycol 23:67–71
Ferroni L, Klisch M, Pancaldi S, Häder DP (2010) Complementary UV-absorption of mycosporine-like amino acids and scytonemin is responsible for the UV-insensitivity of photosynthesis in Nostoc flagelliforme. Mar Drugs 8:106–121
Gao K (1998) Chinese studies on the edible blue-green alga, Nostoc flagelliforme: a review. J Appl Phycol 10:37–49
Gao K, Ye C (2007) Photosynthetic insensitivity of the terrestrial cyanobacterium Nostoc flagelliforme to solar UV radiation while rehydrated or desiccated. J Phycol 43:628–635
Garcia-Pichel F, Sherry ND, Castenholz RW (1992) Evidence for an ultraviolet sunscreen role of the extracellular pigment scytonemin in the terrestrial cyanobacterium Chiorogloeopsis sp. Photochem Photobiol 56:17–23
Garcia-Pichel F, Wingard CE, Castenholz RW (1993) Evidence regarding the UV sunscreen role of a mycosporine-like compound in the cyanobacterium Gloeocapsa sp. Appl Environ Microbiol 59:170–176
Han T, Sinha RP, Hader DP (2003) Effects of intense PAR and UV radiation on photosynthesis, growth and pigmentation in the rice-field cyanobacterium Anabaena sp. Photochem Photobiol Sci 2:649–654
Hall DO, Rao KK (1994) Photosynthesis, 5th edn. Cambridge University Press, Cambridge
Klisch M, Sinha RP, Richter PR, Häder DP (2001) Mycosporine-like amino acids (MAAs) protect against UV-B-induced damage in Gyrodinium dorsum Kofoid. J Plant Physiol 158:1449–1454
Latifi A, Ruiz M, Zhang CC (2009) Oxidative stress in cyanobacteria. FEMS Microbiol Rev 33:258–278
Liu Y, Li X, Jia R, Huang L, Zhou Y, Gao Y (2011) Effects of biological soil crusts on soil nematode communities following dune stabilization in the Tengger Desert Northern China. Appl Soil Ecol 49:118–124
Mandal S, Rath J, Adhikary SP (2011) Adaptation strategies of the sheathed cyanobacterium Lyngbya majuscula to ultraviolet-B. J Photochem Photobiol B: Biol 102:115–122
Pu AP, Song QR, Wang L (2005) A preliminary study of ultraviolet B radiation in the plain region of Ningxia. J of Ningxia Med College 27:297–299
Rastogi RP, Sinha R, Singh SP, Hader DP (2010) Photoprotective compounds from marine organisms. J Ind Microbiol Biotechno 37:537–558
Rath J, Adhikary S (2007) Response of the estuarine cyanobacterium Lyngbya aestuarii to UV-B radiation. J Appl Phycol 19:529–536
Scherer S, Chen TW, Boger P (1988) A New UV-A/B protecting pigment in the terrestrial cyanobacterium Nostoc commune. Plant Physiol 88:1055–1057
Singh S, Sinha R, Klisch M, Hader DP (2008) Mycosporine-like amino acids (MAAs) profile of a rice-field cyanobacterium Anabaena doliolum as influenced by PAR and UVR. Planta 229:225–233
Vass I, Sass L, Spetea C, Bakou A, Ghanotakis DF, Petrouleas V (1996) UV-B-induced inhibition of photosystem II electron transport studied by EPR and chlorophyll fluorescence. Impairment of donor and acceptor side components. Biochem 35:8964–8973
Wang FX, Song QR (2007) Monitoring on the intensity of UVB radiation on different underlying ground in Yinchuan. Modern Preventive Medicine 34:4207–4208
Wang W, Liu Y, Li D, Hu C, Rao B (2009) Feasibility of cyanobacterial inoculation for biological soil crusts formation in desert area. Soil Biol Biochem 41:926–929
Whitehead K, Hedges JI (2005) Photodegradation and photosensitization of mycosporine-like amino acids. J Photochem Photobiol B: Biol 80:115–121
Xie Z, Wang Y, Liu Y, Liu Y (2009) Ultraviolet-B exposure induces photo-oxidative damage and subsequent repair strategies in a desert cyanobacterium Microcoleus vaginatus Gom. Eur J Soil Biol 45:377–382
Yu H, He Y. (2011) The microstructure and formation of artificial biological crusts of cyanobacteria Nostoc flagelliforme on fine sands. Int. Conf. Bioinformatics Biomedical Eng., ICBBE:1–3
Yu HF, Jia SR, Dai YJ (2009) Growth characteristics of the cyanobacterium Nostoc flagelliforme in photoautotrophic, mixotrophic and heterotrophic cultivation. J Appl Phycol 21:127–133
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This work was supported by the National Natural Science Foundation of China (no. 20806047), China Postdoctoral Science Foundation (no. 20090450116), and Excellent Middle-Aged and Young Scientist Award Foundation of Shandong Province (no. BS2011SW029)
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Yu, H., Liu, R. Effect of UV-B radiation on the synthesis of UV-absorbing compounds in a terrestrial cyanobacterium, Nostoc flagelliforme . J Appl Phycol 25, 1441–1446 (2013). https://doi.org/10.1007/s10811-013-9978-6
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DOI: https://doi.org/10.1007/s10811-013-9978-6