Skip to main content
SpringerLink
Log in

Fucose-containing sulfated polysaccharides from brown macroalgae Lobophora variegata with antioxidant, anti-inflammatory, and antitumoral effects

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

This study analyzed sulfated polysaccharides, such as fucans, from the brown alga Lobophora variegata to verify their antioxidant activity in vitro, antitumoral effect on human colon adenocarcinoma cell line HT-29, and anti-inflammatory activity. Sulfated polysaccharide fractions containing fucans were obtained after fractionation with increasing volumes (v) of acetone (0.3–2.0 v). The polysaccharide was eluted with 1.5 v of acetone and named F1.5. The results showed that F1.5 contained a high yield. Chemical and structure analyses were performed by infrared spectroscopy, electrophoresis in agarose gel, and chemical dosages (sugar, protein, phenolic compounds, and sulfate). We observed that this sulfated polysaccharide had antioxidant activity and antitumoral effect. Anti-inflammatory activity in vivo of F1.5 was observed in the croton oil mouse-ear model at 75 mg kg-1. The results were correlated with histopathological analysis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Alban S, Schauerte A, Franz G (2002) Anticoagulant sulfated polysaccharides: Part I. Synthesis and structure-activity relationships of new pullulan sulfates. Carbohydr Polym 47:267–276

    Article  CAS  Google Scholar 

  • Alves MGFC, Nobre LTDB, Monteiro NKV, Moura GEDD, Dore CMPG, Medeiros VP, Leite EL (2011) Effects of heparinoids from algae on hemostasis and their action on the cycle cell. Biomed Prev Nutr 2:163–168

    Google Scholar 

  • Alves MGFC, Dore CMPG, Castro AJG, Nascimento MS, Cruz AKM, Soriano EM, Benevides NMB, Leite EL (2012) Antioxidant, cytotoxic and hemolytic effects of sulfated galactans from edible red alga Hypnea musciformis. J Appl Phycol 24:1217–1227

    Article  CAS  Google Scholar 

  • Arnold TM, Targett NM, Tanner CE, Hatch WI, Ferrari KE (2001) Evidence for methyl jasmonate-induced phlorotannin production in Fucus Vesiculosus (Phaeophyceae). J Phycol 37:1026–1029

    Google Scholar 

  • Athukorala Y, Kim K, Jeon Y (2006) Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem Toxicol 44:1065–1074

    Article  CAS  PubMed  Google Scholar 

  • Azevedo TCG, Bezerra MEB, Santos MGL, Souza LA, Marques CT, Benevides NMB, Leite EL (2009) Heparinoids algal and their anticoagulant, hemorrhagic activities and platelet aggregation. Biomed Pharmacother 63:477–483

    PubMed  Google Scholar 

  • Bernardi G, Springer GF (1962) Properties of highly purified fucan. J Biol Chem 237:75–80

    CAS  PubMed  Google Scholar 

  • Berteau O, Mulloy B (2003) Sulfated fucans, fresh perspective: structures, functions and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide. Glycobiology 13:29–40

    Article  Google Scholar 

  • Bilan MI, Grachev AA, Ustuzhanina NE, Shashkov AS, Nifantiev NE, Usov AI (2007) Structure of a highly pyruvylated galactan sulfate from the Pacific green alga Codium yezoense (Bryopsidales, Chlorophyta). Carbohydr Res 342:586–596

    Article  CAS  PubMed  Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Chattopadhyay N, Ghosh T, Sinha S, Chattopadhyay K, Karmakar P, Ray B (2010) Polysaccharides from Turbinaria conoides: structural features and antioxidant capacity. Food Chem 118/3:823–829

    Article  CAS  Google Scholar 

  • Cumashi A, Ushakov NA, Preobrazhenskaya ME, D'Incecco A, Piccoli A, Totani L, Tinari N, Morozevich GE, Berman, AE, Bilan MI, Usov, AI, Ustyuzhanina NE, Grachev AA, Sanderson, CJ, Kelly M, Rabinovich GA, Iacobelli S, Nifantiev NE (2007) A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology 17541–1552

  • Dietrich CP, Farias GGM, Abreu LRD, Silva LF, Leite EL, Nader HB (1995) A new approach for characterization of polysaccharides from algae: presence of four main acidic polysaccharides in three species of the class Phaeophyceae. Plant Sci 108:143–153

    Article  CAS  Google Scholar 

  • Dodgson KS, Price RG (1962) A note on the determination of ester sulphate content of sulphated polysaccharides. Biochem J 84:106–110

    CAS  PubMed Central  PubMed  Google Scholar 

  • Dore CM, Faustino d C, Alves MG, Will LS, Costa TG, Sabry DA, de Souza Rêgo LA, Accardo CM, Rocha HA, Filgueira LG, Leite EL (2013) A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects. Carbohydr Polym 91:467–475

    Article  CAS  PubMed  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Gomez-Ordonez E, Ruperez P (2011) FTIR-ATR Spectroscopy as a tool for polysaccharide identification in edible brown and red seaweeds. Food Hydrocolloids 25:1514–1520

    Article  CAS  Google Scholar 

  • Jiao G, Yu G, Zhang J, Ewart HS (2011) Chemical structures and bioactivities of sulfated polysaccharides from marine algae. Mar Drugs 9:196–223

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Karmakar P, Ghosh T, Sinha S, Saha S, Pinak M (2009) Polysaccharides from the brown seaweed Padina tetrastromatica: characterization of a sulfated fucan. Carbohydr Polym 78:416–421

    Article  CAS  Google Scholar 

  • Kuda T, Kunaii T, Gota H, Suziki T, Yano T (2007) Varieties of antioxidant and antibacterial properties of Ecklonia stolonifera and Ecklonia kurome products harvested and processed in the Noto peninsula. Jap Food Chem 103:900–905

    Article  CAS  Google Scholar 

  • Leite EL, Medeiros MGL, Rocha HAO, Farias GGM, Silva LF, Chavante SF, Abreu LD, Dietrich CP, Nader HB (1998) Structure and pharmacological activities of a sulfated xylofucoglucuronan from the alga Spatoglossum schroederi. Plant Sci 132:215–228

    Article  CAS  Google Scholar 

  • Marques CT, Azevedo TCG, Nascimento MS, Medeiros VP, Alves LG, Benevides NMB, Rocha HAO, Leite EL (2012) Sulfated fucans extracted from algae Padina gymnospora have anti-inflammatory effect. Braz J Pharm 122:115–122

    Google Scholar 

  • Medeiros VP, Queiroz KC, Cardoso ML, Monteiro GR, Oliveira FW, Chavante SF, Guimaraes LA, Rocha HA, Leite EL (2008) Sulfated galactofucan from L. variegata: anticoagulant and anti-inflammatory properties. Biochemistry (Mosc) 73:1018–1024

    Article  CAS  Google Scholar 

  • Mišurcová L, Ambrožová J, Samek D (2011) Seaweed lipids as nutraceuticals. Adv Food Nutr Res 64:339–355

    Article  PubMed  Google Scholar 

  • Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63

    Article  CAS  PubMed  Google Scholar 

  • Paiva OAA, Castro AJG, Nascimento MS, Will LSEP, Santos ND, Araújo RM, Xavier CAC, Rocha FA, Leite EL (2011) Antioxidant and anti-inflammatory effect of polysaccharides from Lobophora variegata on zymosan-induced arthritis in rats. Int Immunopharm 11:1241–1250

    Article  CAS  Google Scholar 

  • Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 269:337–341

    Article  CAS  PubMed  Google Scholar 

  • Satarelli R, Ceccaroli P, Lotti M, Zambonelli A, Buffalini M, Casadei L, Vallorani L, Stocchi V (2009) Biochemical characterization and antioxidant activity of mycelium of Ganoderma lucidum from Central Italy. Food Chem 116:143–151

    Article  Google Scholar 

  • Silva TMA, Alves LG, Queiroz KCS, Santos MGL, Marques CT, Chavante SF, Rocha HAO, Leite EL (2005) Partial characterization and anticoagulant activity of a heterofucan from the brown seaweed Padina gymnospora. Braz J Med Biol 38:523–533

    Google Scholar 

  • Smirnoff N, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28:1057–1060

    Article  CAS  Google Scholar 

  • Souza MCR, Marques CT, Dore CMPG, Silva FRF, Rocha HAO, Leite EL (2007) Antioxidant activities of sulfated polysaccharides from brown and red seaweeds. J Appl Phycol 19:153–160

    Article  Google Scholar 

  • Swain T, Hills WE (1959) The phenolic constituents of Prunus domestica: I. The quantitative analysis of phenolic constituents. J Sci Food Agr 10:63–68

    Article  CAS  Google Scholar 

  • Targett NM, Arnold TM (1998) Predicting the effects of brown algal phlorotannins on marine herbivores in tropical and temperate oceans. J Phycol 34:195–205

    Google Scholar 

  • Targett NM, Boettcher AA, Targett TE, Vrolijk NH (1995) Tropical marine herbivore assimilation of phenolic-rich plants. Oecologia 103:170–179

    Google Scholar 

  • Wang J, Zhang Q, Zhang Z, Li Z (2008) Antioxidant activity of sulfated polysaccharide fractions extracted from Laminaria japonica. Int J Biol Macromol 42:127–132

    Article  PubMed  Google Scholar 

  • Wijesekara I, Kim S (2010) Angiotensin-I-converting enzyme (ACE) inhibitors from marine resources: Prospects in the pharmaceutical industry. Mar Drugs 8:1080–1093

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Wijesekara I, Pangestuti R, Kim S (2011) Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carbohydr Polym 84:14–21

    Article  CAS  Google Scholar 

  • Wolfe K, Wu X, Liu RH (2003) Antioxidant activity of apple peels. J Agric Food Chem 21:609–614

    Article  Google Scholar 

  • Ye H, Wang K, Zhou C, Liu J, Zeng X (2008) Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chem 111:428–432

    Article  CAS  Google Scholar 

Download references

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Laboratory of Glicobiology, Department of Biochemistry, Federal University of Rio Grande do Norte, Av. Sen. Salgado Filho, 3000, Lagoa Nova, Natal, Rio Grande do Norte, Brazil

    Luiza Sheyla E. P. Will Castro, Thuane S. Pinheiro, Allisson J. G. Castro, Celina M. P. G. Dore, Monique Gabriela das C. Faustino Alves, Marilia S. Nascimento Santos & Edda Lisboa Leite

  2. Department of Histology, Federal University of Rio Grande do Norte, Av. Sen. Salgado Filho, 3000, Lagoa Nova, Natal, Rio Grande do Norte, Brazil

    Naissandra B. da Silva

  3. Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte, 59082-970, Natal, Rio Grande do Norte, Brazil

    Edda Lisboa Leite

Authors
  1. Luiza Sheyla E. P. Will Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thuane S. Pinheiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Allisson J. G. Castro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Celina M. P. G. Dore
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Naissandra B. da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Monique Gabriela das C. Faustino Alves
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Marilia S. Nascimento Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Edda Lisboa Leite
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Edda Lisboa Leite.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Castro, L.S.E.P.W., Pinheiro, T.S., Castro, A.J.G. et al. Fucose-containing sulfated polysaccharides from brown macroalgae Lobophora variegata with antioxidant, anti-inflammatory, and antitumoral effects. J Appl Phycol 26, 1783–1790 (2014). https://doi.org/10.1007/s10811-013-0217-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10811-013-0217-y

Keywords

Search

Navigation