Abstract
The effects of organic carbon sources on cell growth and exopolysaccharide (EPS) production of dissociated Nostoc flagelliforme cells under mixotrophic batch culture were investigated. After 7 days of cultivation, glycerol, acetate, sucrose, and glucose increased the final cell density and final EPS concentrations, and mixotrophic growth achieved higher biomass concentrations. The increase in cell growth was particularly high when glucose was added as the sole carbon source. On the other hand, EPS production per dry cell weight was significantly enhanced by adding acetate. For more effective EPS production, the effects of the mixture of glucose and acetate were investigated. Increasing the ratio of glucose to acetate resulted in higher growth rate with BG-11 medium and higher EPS productivity with BG-110 medium (without NaNO3). When the medium was supplemented with a mixture of glucose (4.0 g L−1) and acetate (2.0 g L−1), 1.79 g L−1 biomass with BG-11 medium and 879.6 mg L−1 of EPS production with BG-110 medium were achieved. Adopting this optimal ratio of glucose to acetate established in flask culture, the culture was also conducted in a 20-L photobioreactor with BG-11 medium for 7 days. A maximum biomass of 2.32 g L−1 was achieved, and the EPS production was 634.6 mg L−1.
Similar content being viewed by others
References
Allen MM, Stanier RY (1968) Growth and division of some unicellular blue-green algae. J Gen Microbiol 51:199–202
Bai XJ, Su JY, Zhao SX, Jia SR (2004) Study on the determination methods of extracellular polysaccharide in culture medium of Nostoc flagelliforme cells. Sci Techno Food Industry 25:146–148 (in Chinese with English abstract)
Bi YH, Hu ZY (2004) Influence of temperature, nutrients and light intensity on the growth of Nostoc flagelliforme. Chin J Process Eng 4:245–249 (in Chinese with English abstract)
Chen TF, Zheng WJ, Fang Y, Bai Y, Wang YS (2006) Mixotrophic culture of high selenium-enriched Spirulina platensis on acetate and the enhanced production of photosynthetic pigments. Enzyme Microb Technol 39:103–107
Dai ZJ (1992) Review of Nostoc flagelliforme research. J Ningxia Univ Nat Sci Ed 1:71–77 (in Chinese with English abstract)
Gao KS, Ye CP (2003) Culture of the terrestrial cyanobacterium, Nostoc flagelliforme (Cyanophyceae), under aquatic conditions. J Phycol 39:617–623
Jia SR, Yu HF, Lin YX, Dai YJ (2007) Characterization of extracellular polysaccharides from Nostoc flagelliforme cells in liquid suspension culture. Biotecnol Bioprocess Eng 12:271–275
Kanekiyo K, Le JB, Hayashi K, Takenaka H, Hayakawa Y, Endo S, Hayashi T (2005) Isolation of an antiviral polysaccharide, Nostoflan, from a terrestrial cyanobacterium, Nostoc flagelliforme. J Nat Prod 68:1037–1041
Kanekiyo K, Hayashi K, Takenaka H, Lee JB, Hayashi T (2007) Anti-herpes simplex virus target of an acidic polysaccharide, Nostoflan, from the edible blue-green alga Nostoc flagelliforme. Biol Pharm Bull 30:1573–1575
Kanekiyo K, Hayashi K, Lee JB, Takenaka H, Hayashi T (2008) Structure and antiviral activity of an acidic polysaccharide from an edible blue-green alga Nostoc flagelliforme Yakugaku Zasshi 128:725–731
Kang RJ, Wang J, Shi DJ, Cong W, Cai ZL, Ouyang F (2004) Interactions between organic and inorganic carbon sources during mixotrophic cultivation of Synechococcus sp. Biotechnol Lett 26:1429–1432
Kumar AS, Mody K, Jha B (2007) Bacterial exopolysaccharides—a perception. J Basic Microb 47:103–117
Liu XJ, Chen F (2003) Cell differentiation and colony alteration of an edible terrestrial cyanobacterium Nostoc flagelliforme, in liquid suspension cultures. Folia Microbiol 48:619–626
Pereira S, Zille A, Micheletti E, Moradas FP, De Philippis R, Tamagnini P (2009) Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiol Rev 33:917–941
Su JY, Jia SR, Chen XF, Yu HF (2008) Morphology, cell growth, and polysaccharide production of Nostoc flagelliforme in liquid suspension culture at different agitation rates. J Appl Phycol 20:213–217
Sutherland IW (1998) Novel and established applications of microbial polysaccharides. Trends Biotechnol 16:41–46
Takenaka H, Sumiya T, Ito H (1997) Effects of hot water extract prepared from Nostoc flagelliforme on macrophage activities in tumor-bearing mice. Med Biol 135:231–234
Tamaru Y, Takani Y, Yoshida T, Sakamoto T (2005) Crucial role of extracellular polysaccharides in desiccation and freezing tolerance in the terrestrial cyanobacterium Nostoc commune. Appl Environ Microbiol 71:7327–7333
Yu HF, Jia SR, Dai YJ (2009) Growth characteristics of the cyanobacterium Nostoc flagelliforme in photoautotrophic, mixotrophic and heterotrophic cultivation. J Appl Phycol 1:127–133
Zhao XM, Bi YH, Hu ZY (2005) Effects of different medium on the growth and photosynthetic activity of Nostoc flagelliforme. J Wuhan Bot Res 23:332–335
Acknowledgment
This work was supported by the National Natural Science Foundation of China (no. 20806047) and by China Postdoctoral Science Foundation (no. 20090450116).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yu, H. Effect of mixed carbon substrate on exopolysaccharide production of cyanobacterium Nostoc flagelliforme in mixotrophic cultures. J Appl Phycol 24, 669–673 (2012). https://doi.org/10.1007/s10811-011-9684-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10811-011-9684-1