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A simple method for electroanalytical determination of ceftiofur in UHT milk samples using square-wave voltammetry technique

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Abstract

A simple electroanalytical method was developed to determine the antibiotic ceftiofur (CF) in milk. The method is based on the adsorptive accumulation of the drug on a hanging mercury-drop electrode (HMDE) and the accompanying initiation of a negative square wave, which yielded well-defined cathodic peaks at −0.60 V (1C) and −0.91 V (2C) vs. Ag/AgCl. Calibration graphs were constructed and statistical parameters were evaluated. At pH 2.5, the square-wave voltammetry method revealed linearity from 52.4 to 524 ng mL−1 (r = 0.997), which is in accordance with the tolerance level of 100 ng mL−1 for CF as a residue in bovine milk established by the Food and Drug Administration (FDA) and the European Union. The limits of detection and quantification were 1.86 and 6.20 ng mL−1, respectively. The method was tested to determine CF in spiked milk samples using HPLC as reference method.

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Acknowledgments

The authors gratefully acknowledge the financial support provided by CAPES, CNPq, and FUNDECT and the generous gift by Bayer of the ceftiofur drug.

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Authors and Affiliations

  1. Department of Chemistry, Universidade Federal de Mato Grosso do Sul (UFMS), C.P. 549, Campo Grande, MS, 79074-460, Brazil

    Antonio Marcos Jacques Barbosa, Tatiane Alfonso de Araújo, Magno Aparecido Gonçalves Trindade & Valdir Souza Ferreira

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  1. Antonio Marcos Jacques Barbosa
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  2. Tatiane Alfonso de Araújo
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  3. Magno Aparecido Gonçalves Trindade
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  4. Valdir Souza Ferreira
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Correspondence to Magno Aparecido Gonçalves Trindade.

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Barbosa, A.M.J., de Araújo, T.A., Trindade, M.A.G. et al. A simple method for electroanalytical determination of ceftiofur in UHT milk samples using square-wave voltammetry technique. J Appl Electrochem 41, 249–255 (2011). https://doi.org/10.1007/s10800-010-0232-5

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  • DOI: https://doi.org/10.1007/s10800-010-0232-5

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