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Metabolomics analysis in pterygium tissue

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Abstract

Purpose

The aim of the study was to measure amino acid levels with the metabolomics analysis in pterygium tissue and normal conjunctiva tissue.

Materials and methods

In this prospective, randomized, clinical study, a comparison of the amino acid profile of pterygium tissue and normal conjunctiva tissue taken during autograft pterygium surgery was made. After homogenization of the tissues, amino acid levels were measured with chromatography–mass spectrometry (LC–MS/MS) in the biochemistry laboratory. Statistical analysis was made using the Wilcoxon signed-rank test.

Results

Evaluation of pterygium and normal conjunctiva tissues of 29 patients, comprising 16 females and 13 males with a mean age of 54.75 ± 11.25 years (range 21–78 years) was made. While a dramatic increase was observed in all the amino acid levels in the pterygium tissue compared to the normal conjunctiva (p > 0.05), only the increases in arginine, methionine, glycine and tyrosine amino acids were determined to be statistically significant (p < 0.01), (p = 0.028), (p = 0.038), (p = 0.046).

Conclusion

Pterygium is known to be degenerative inflammatory fibrovascular tissue. When the aetiology is examined in depth, several metabolic processes are seen to have an effect. Further studies of the amino acid profile with more extensive patient series could confirm the data obtained in the current study and contribute to the clarification of the pathogenesis of pterygium.

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Funding

This work was supported by the research fund of Harran University (HUBAK). Project Number: 17244.

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Authors and Affiliations

  1. Department of Ophthalmology, Harran University Faculty of Medicine, 63300, Haliliye, Şanlıurfa, Turkey

    Ayhan Saglik, Fatih Mehmet Adibelli & Muslum Toptan

  2. Department of Biochemistry, Harran University Faculty of Medicine, Haliliye, Şanlıurfa, Turkey

    Ismail Koyuncu & Ataman Gonel

  3. Unit of Biometry and Genetics, Harran University Faculty of Agriculture, Haliliye, Şanlıurfa, Turkey

    Hamza Yalcin

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  1. Ayhan Saglik
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  2. Ismail Koyuncu
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Correspondence to Ayhan Saglik.

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All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership or other equity interest; and expert testimony or patent-licensing arrangements) or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

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Saglik, A., Koyuncu, I., Gonel, A. et al. Metabolomics analysis in pterygium tissue. Int Ophthalmol 39, 2325–2333 (2019). https://doi.org/10.1007/s10792-018-01069-2

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  • DOI: https://doi.org/10.1007/s10792-018-01069-2

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