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Effects of methanolic extract from leaves of Rubus imperialis in DSS-induced colitis in mice

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Abstract

This study investigated the effects of Rubus imperialis, a berry known as “amora-branca”, in colitis dextran sulfate sodium (DSS)-induced in mice. Animals were treated orally with vehicle (water), 5-aminosalicylic acid (100 mg/kg) or methanolic extract from leaves of R. imperialis (MERI, 100 mg/kg), once a day during seven days. The disease activity index (DAI) was observed daily. Colons were collected for histological, histochemical and biochemical analysis. The administration of MERI exacerbated colitis, as indicated by DAI heightened weight loss and increased histological colonic injury. MERI also decreased the colon mucin levels and increased colonic TNF content. The colonic levels of reduced glutathione and the superoxide dismutase activity in colitic group treated with MERI were decreased. Despite the worsening of colitis, MERI not altered the intestinal transit, body weight, colon length or organs weight in normal mice. Tormentic acid (TA) and 2β,3β,19α-trihydroxyursolic acid (THA), compounds isolated from MERI, reduced the L929 cells viability. Thus, MERI may have aggravated the DSS-induced colitis through intense intestinal mucus barrier impairment, which would lead to inflammatory responses, TA and THA contribute to the intestinal damage verified suggesting caution about the use of R. imperialis preparations, particularly in inflammatory bowel diseases.

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Acknowledgements

LBS and TB acknowledge the fellowships from CNPq/Brazil, and UNIVALI/Brazil. This work was supported by CNPq, FAPESC, and CAPES.

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Authors and Affiliations

  1. Programa de Pós-Graduação em Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade do Vale do Itajaí, Rua Uruguai, 458, Centro, Itajaí, SC, 88302-901, Brazil

    Luisa Mota da Silva, Lincon Bordignon Somensi, Thaise Boeing, Cristiane Barp, Valdir Cechinel-Filho, Rivaldo Niero & Sérgio Faloni de Andrade

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  1. Luisa Mota da Silva
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  2. Lincon Bordignon Somensi
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  3. Thaise Boeing
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  4. Cristiane Barp
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  5. Valdir Cechinel-Filho
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  6. Rivaldo Niero
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  7. Sérgio Faloni de Andrade
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Correspondence to Luisa Mota da Silva.

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The authors declare that they have no conflict of interest.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted.

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da Silva, L.M., Somensi, L.B., Boeing, T. et al. Effects of methanolic extract from leaves of Rubus imperialis in DSS-induced colitis in mice. Inflammopharmacol 24, 403–409 (2016). https://doi.org/10.1007/s10787-016-0293-0

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  • DOI: https://doi.org/10.1007/s10787-016-0293-0

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