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Cudraflavanone B Isolated from the Root Bark of Cudrania tricuspidata Alleviates Lipopolysaccharide-Induced Inflammatory Responses by Downregulating NF-κB and ERK MAPK Signaling Pathways in RAW264.7 Macrophages and BV2 Microglia

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Abstract

A prenylated flavonoid, cudraflavanone B, is isolated from Cudrania tricuspidata. In this study, we investigated its anti-inflammatory and anti-neuroinflammatory effects in lipopolysaccharide (LPS)-induced RAW264.7 and BV2 cells. In our initial study of the anti-inflammatory effects of cudraflavanone B the production of nitric oxide and prostaglandin E2 was attenuated in LPS-stimulated RAW264.7 and BV2 cells. These inhibitory effects were related to the downregulation of inducible nitric oxide synthase and cyclooxygenase-2. In addition, cudraflavanone B suppressed the production of pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-α in LPS-induced RAW264.7 and BV2 cells. Moreover, the evaluation of the molecular mechanisms underlying the anti-inflammatory effects of cudraflavanone B revealed that the compound attenuated the nuclear factor-kappa B signaling pathway in LPS-induced RAW264.7 and BV2 cells. In addition, cudraflavanone B inhibited the phosphorylation of extracellular signal-regulated kinase mitogen-activated protein kinase signaling pathways in these LPS-stimulated cells. Thus, cudraflavanone B suppressed nuclear factor-κB, and extracellular signal-regulated kinase mitogen-activated protein kinase mediated inflammatory pathways, demonstrating its potential in the treatment of neuroinflammatory conditions.

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The data and material used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

The current study was supported by a research fund from Chosun University in 2016 (K207334002).

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  1. Wonmin Ko and Kwan-Woo Kim contributed equally to this work.

Authors and Affiliations

  1. College of Pharmacy, Chosun University, Dong-gu, Gwangju, 61452, Republic of Korea

    Wonmin Ko, Nayeon Kim, Hwan Lee, Eun-Rhan Woo & Dong-Sung Lee

  2. College of Pharmacy, Wonkwang University, Iksan, 54538, Republic of Korea

    Kwan-Woo Kim, Youn-Chul Kim & Hyuncheol Oh

  3. Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam

    Tran Hong Quang

  4. Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju-si, 28116, Republic of Korea

    Chi-Su Yoon

  5. Department of Family Practice and Community Medicine, Chosun University College of Medicine, Dong-gu, Gwangju, 61452, Republic of Korea

    Sam-Cheol Kim

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  1. Wonmin Ko
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Ko, W., Kim, KW., Quang, T.H. et al. Cudraflavanone B Isolated from the Root Bark of Cudrania tricuspidata Alleviates Lipopolysaccharide-Induced Inflammatory Responses by Downregulating NF-κB and ERK MAPK Signaling Pathways in RAW264.7 Macrophages and BV2 Microglia. Inflammation 44, 104–115 (2021). https://doi.org/10.1007/s10753-020-01312-y

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