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Downregulation of Aquaporins (AQP1 and AQP5) and Na,K-ATPase in Porcine Reproductive and Respiratory Syndrome Virus-Infected Pig Lungs

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Abstract

Aquaporins (AQPs) and Na,K-ATPase control water transport across the air space–capillary barrier in the distal lung and play an important role in the formation and resolution of lung edema. Porcine reproductive and respiratory syndrome virus (PRRSV) infection usually causes pulmonary inflammation and edema in the infected pig lungs. To investigate the possibility that PRRSV infection may cause altered expression of AQPs and Na,K-ATPase messenger RNA (mRNA) levels and protein expression of AQP1, AQP5, and Na,K-ATPase in the PRRSV-infected pig lungs were detected. Quantitative real-time PCR (qRT-PCR) analysis showed markedly decreased mRNA levels of AQP1 and AQP5 and Na,K-ATPase in the PRRSV-infected pig lungs compared to those of uninfected pig lungs. Western blot studies also revealed significantly reduced levels of AQP1, AQP5, and Na,K-ATPase proteins in the PRRSV-infected pig lungs. In addition, immunohistochemical (IHC) analysis showed decreased protein expression of AQP1 and AQP5 in the endothelial cells of the capillaries and venules and secretory cells of terminal bronchiole and the alveolar type I cells, respectively. The expression of Na,K-ATPase in the basolateral membrane of alveolar type II cells presented great reduction in the PRRSV-infected pig lungs. To further understand the reduction of these proteins, the ubiquitination of AQP1 and Na,K-ATPase was examined in uninfected and PRRSV-infected pig lungs. The results showed that there is no difference of ubiquitination for these proteins. Thus, our results suggest that PRRSV infection may induce downregulation of these proteins and cause impairment of edema resolution by failed water clearance in the infected pig lungs.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC) Grants 31372418 (G. Liu) and 31460563 (J. Zhang), Huazhong Agricultural University Scientific and Technology Self-Innovation Foundation Program 2012RC011 (G. Liu), and the 948 Project of Chinese Ministry of Agriculture (2015-Z33, GL).

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Authors and Affiliations

  1. Department of Basic Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People’s Republic of China

    Jianping Zhang, Wei Gu, Ao Chen, Jie Liu, Lexing Li & Guoquan Liu

  2. College of Life Science, Tarim University, Alar, Xinjiang Province, 843300, People’s Republic of China

    Jianping Zhang

  3. Key Lab of Biological Resources Protection and Utilization in Tarim Basin, Tarim University, Alar, Xinjiang Province, 843300, People’s Republic of China

    Jianping Zhang

  4. Key Lab of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan Province, 646000, People’s Republic of China

    Meiping Yan

  5. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou, Shandong Province, 256600, People’s Republic of China

    Songlin Zhang

  6. Key Lab of Swine Genetics and Breeding and Agricultural Animal Breeding and Reproduction, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province, 430070, People’s Republic of China

    Guoquan Liu

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  1. Jianping Zhang
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Correspondence to Guoquan Liu.

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The animal experiments were approved by the Animal Care and Use Committee of Hubei Province, China, in accordance with guidelines developed by the China Council on Animal Care and protocol.

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Zhang, J., Yan, M., Gu, W. et al. Downregulation of Aquaporins (AQP1 and AQP5) and Na,K-ATPase in Porcine Reproductive and Respiratory Syndrome Virus-Infected Pig Lungs. Inflammation 41, 1104–1114 (2018). https://doi.org/10.1007/s10753-018-0762-2

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