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Genetic Polymorphisms of X-ray Repair Cross-Complementing Group 1 and Apurinic/Apyrimidinic Endonuclease-1 in Chronic Obstructive Pulmonary Disease

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Abstract

Chronic obstructive pulmonary disease (COPD) is a heterogeneous collection of conditions characterized by irreversible expiratory airflow limitation. The disease is interspersed with exacerbations; periods of acute symptomatic, physiological, and functional deterioration. The present study was designed to investigate the role of X-ray cross-complementing group 1 (XRCC1) and apurinic/apyrimidinic endonuclease 1 (APE1) polymorphisms and the risk of COPD. Blood samples from 354 unrelated subject (age range 18–60 years; 156 with COPD, 198 healthy controls) were collected. Genomic DNA was isolated and genotyped for XRCC1 Arg399Gln and APE1 Asp148Glu using a confronting two pair primers polymerase chain reaction. GA genotype of XRCC1 gene was found to be predominant in the COPD group compared to controls with 1.86-fold increased risk for COPD (OR 1.86, 95 % CI 1.20–2.88, p = 0.0013). TG genotype of APE1 was found to be predominant in COPD group compared to controls with the difference being statistically significant (OR 1.68, 95 % CI 1.08–2.61, p = 0.0043). The GA haplotype was found to be predominant in COPD than controls with a 2.19-fold significant increase (OR 2.19, 95 % CI 1.46–3.28, p = 0.003). Polymorphism in XRCC1 and APE1 gene is associated with an increased risk of COPD.

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Acknowledgments

The study was not supported by any intramural or extramural funds.

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Authors and Affiliations

  1. Central Laboratory for Stem Cell Research & Translational Medicine, Centre for Liver Research and Diagnostics, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500 058, Andhra Pradesh, India

    Avinash Bardia, Sandeep Kumar Vishwakarma, Chandrakala Lakki Reddy, N. Raju, Shaik Iqbal, Gallapalli Sravani, Narneni Lavanya, Nazima Begum & Aleem A. Khan

  2. Department of Physiology, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500 058, Andhra Pradesh, India

    Naziya Usma

  3. Department of Genetics, Osmania University, Hyderabad, 500 007, Andhra Pradesh, India

    Pratibha Nallari

  4. Department of Biochemistry, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500 058, Andhra Pradesh, India

    Baderuzzaman

  5. Department of Pulmonology, Owaisi Hospital & Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Hyderabad, 500 058, Andhra Pradesh, India

    Syed Mehmood Ahmed & Asfaq Hasan

Authors
  1. Avinash Bardia
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  2. Sandeep Kumar Vishwakarma
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Correspondence to Aleem A. Khan.

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Bardia, A., Vishwakarma, S.K., Reddy, C.L. et al. Genetic Polymorphisms of X-ray Repair Cross-Complementing Group 1 and Apurinic/Apyrimidinic Endonuclease-1 in Chronic Obstructive Pulmonary Disease. Inflammation 39, 1198–1204 (2016). https://doi.org/10.1007/s10753-016-0355-x

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  • DOI: https://doi.org/10.1007/s10753-016-0355-x

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