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Preventive and Therapeutic Effects of a Beta Adrenoreceptor Agonist, Dobutamine, in Carrageenan-Induced Inflammatory Nociception in Rats

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Abstract

We hypothesized that locally administrated β-adrenoreceptor agonist can modulate the inflammatory nociceptive parameters in carrageenan (CG)-induced peripheral inflammatory pain. This study was therefore aimed to assess the preventive and therapeutic effects of a β-agonist, dobutamine, by investigating its pretreatment and posttreatment actions on the inflammation-induced hypersensitivities (thermal hyperalgesia, mechanical allodynia) to cutaneous stimulation, edema, and several biochemical oxidant and anti-oxidant parameters in a rat model of CG-induced hind paw inflammation. Effects of dobutamine were compared with those of esmolol, a β-adrenoreceptor antagonist. CG injection to healthy rats lowered the thermal latencies (from 10.1 ± 0.2 to 4.9 ± 0.1 s) and mechanical thresholds (from 32.9 ± 0.5 to 18.9 ± 1.3 g) and caused the hyperalgesia and allodynia. In CG-induced inflamed paws, while intraplantar esmolol treatment (1 mg) produced significant decreases in latencies (4.1 ± 0.1 s) and thresholds (15.2 ± 2.4 g), dobutamine (1 mg) increased the latencies (11.3 ± 0.5 s) and thresholds (26.3 ± 2.8 g). In contrast to esmolol, dobutamine increased the superoxide dismutase level and decreased the myeloperoxidase, malondialdehyde, and nitric oxide levels in CG-induced inflamed paws. The present results can reveal that β-adrenoreceptors may play a role in inflammatory nociceptive processes, and locally treated β-adrenoreceptor agonists such as dobutamine can be a preferable, appropriate choice for the management of inflammatory nociception due to their preventive and therapeutic effects on CG-induced peripheral inflammatory nociception.

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Acknowledgments

This study was supported by the Kahramanmaras Sutcu Imam University Research Foundation (2012/2-12 M).

Conflict of Interest

The authors state no conflict of interest.

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Authors and Affiliations

  1. Department of Biophysics, School of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey

    Tufan Mert

  2. Department of Anatomy, School of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey

    Berin Tugtag

  3. Department of Biochemistry, School of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey

    Metin Kilinc & Elif Sahin

  4. Anaesthesiology and Reanimation, School of Medicine, Kahramanmaras Sutcu Imam University, 46050, Kahramanmaras, Turkey

    Hafize Oksuz

  5. Anaesthesiology and Reanimation, School of Medicine, Cukurova University, 01330, Adana, Turkey

    Yasemin Gunes

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  1. Tufan Mert
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Correspondence to Tufan Mert.

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Mert, T., Tugtag, B., Kilinc, M. et al. Preventive and Therapeutic Effects of a Beta Adrenoreceptor Agonist, Dobutamine, in Carrageenan-Induced Inflammatory Nociception in Rats. Inflammation 37, 1814–1825 (2014). https://doi.org/10.1007/s10753-014-9912-3

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