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Ozonotherapy in an Induced Septic Shock. I. Effect of Ozonotherapy on Rat Organs in Evaluation of Free Radical Reactions and Selected Enzymatic Systems

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Abstract

The confirmed advantageous effects of oxygen/ozone therapy in several clinical conditions stimulated experimental studies on effects of the therapy in rats with an induced septic shock. The studies were conducted on adult male rats of Wistar strain. Four groups of the animals, each of 15 rats, included: I—control group, (C); II—animals intraperitoneally administered with O2/O3 (CO), III—rats given of Escherichia coli endotoxin (lipopolysaccharide—LPS) (CL), IV—rats administered with the lipopolysaccharide plus administered with the oxygen/ozone mixture (OL). Activities of catalase and superoxide dismutase and of free radical reactions were estimated. The exposure to LPS augmented activities of SOD and of catalase in liver, lungs and heart. In all the examined organs LPS induced significant changes in levels of free radicals. Except of the lungs, parallel administration of the rats with LPS and ozone/oxygen revoked development of the alterations. The obtained results point to a strong, stabilizing and regenerative effect of ozonotherapy.

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References

  1. Barriere, S. L., and J. B. Guglielmo. 1992. Gram-negative sepsis, the sepsis syndrome and the role of antiendotoxin monoclonal antibodies. Clin. Pharm. 11:223–232.

    PubMed  CAS  Google Scholar 

  2. Madej, P., Z. Antoszewski, and J. A. Madej. 1995. Ozonotherapy. Mater Med. Pol. 27:53–56.

    PubMed  CAS  Google Scholar 

  3. Laszczyca, P., E. Kawka-Serwecinska, I. Witas, B. Dolezych, B. Falkus, A. Mekail, B. Ziolkowska, P. Madej, and P. Migula. 1996. Lipid peroxidation and activity of antioxidative enzymes in the rat model of ozone therapy. Mater Med. Pol. 28:155–160.

    Google Scholar 

  4. Leon, O. S., S. Menendez, N. Merino, R. Castillo, S. Sam, L. Perez, E. Cruz, and V. Bocci. 1998. Ozone oxidative preconditioning: a protection against cellular damage by free radicals. Mediat. Inflamm. 7:289–294.

    Article  CAS  Google Scholar 

  5. Barber, E., S. Menendez, O. S. Leon, M. O. Barber, N. Merino, J. L. Calunga, E. Cruz, and V. Bocci. 1999. Prevention of renal injury after induction of ozone tolerance in rats submitted to warm ischaemia. Mediat. Inflamm. 8:37–41.

    Article  CAS  Google Scholar 

  6. Fridovich, I. 1976. Superoxide dismutases: studies of structure and mechanism. Adv. Exp. Med. Biol. 74:530–539.

    PubMed  CAS  Google Scholar 

  7. Yim, M. B., P. B. Chock, and E. R. Stadtman. 1993. Enzyme function of copper, zinc superoxide dismutase as a free radical generator. J. Biol. Chem. 268:4099–4105.

    PubMed  CAS  Google Scholar 

  8. Geller, B. L., and D. R. Winge. 1982. Rat liver Cu,Zn-superoxide dismutase. Subcellular location in lysosomes. J. Biol. Chem. 257:8945–8952.

    PubMed  CAS  Google Scholar 

  9. Boveris, A., S. Alvarez, and A. Navarro. 2002. The role of mitochondrial nitric oxide synthase in inflammation and septic shock. Free Radic. Biol. Med. 33:1186–1193.

    Article  PubMed  CAS  Google Scholar 

  10. Hirata, Y., and S. Ishimaru. 2002. Effects of endothelin receptor antagonists on endothelin-1 and inducible nitric oxide synthase genes in a rat endotoxic shock model. Clin. Sci. (Lond) 103(Suppl 48):332S–335S.

    CAS  Google Scholar 

  11. Reade, M. C., and J. D. Young. 2003. Of mice and men (and rats): implications of species and stimulus differences for the interpretation of studies of nitric oxide in sepsis. Br. J. Anaesth. 90:115–118.

    Article  PubMed  CAS  Google Scholar 

  12. Canada, A. T., E. J. Calabrese, and D. Leonard. 1986. Age-dependent inhibition of pentobarbital sleeping time by ozone in mice and rats. J. Gerontol. 41:587–589.

    PubMed  CAS  Google Scholar 

  13. Rilling, S., and R. Viebahn. 1990. Praxis der Ozon-Sauerstoff-Therapie, E. Fischer ed., Verlag fur Medizin, Heidelberg.

  14. Hatch, G., R. Slade, L. Harris, W. McDonnel, R. Devlin, H. Koren, D. Costa, and J. McKee. 1994. Ozone dose and effect in humans and rats. A comparison using oxygen-18 labeling and bronchoalveolar lavage. Am. J. Respir. Crit. Care Med. 150:676–683.

    PubMed  CAS  Google Scholar 

  15. Korbut, R., and J. Gryglewski. 1996. The effect of prostacyclin and nitric oxide on deformability of red blood cells in septic shock in rats. J. Physiol. Pharmacol. 47:591–599.

    PubMed  CAS  Google Scholar 

  16. Jimba, M., W. Skornik, C. Killinsworth, N. Long, J. Brain, and S. Shore. 1995. Role of C fibers in physiological responses to ozone in rats. J. Appl. Physiol. 78:1757–1763.

    Article  PubMed  CAS  Google Scholar 

  17. Verrazzo, G., L. Coppola, C. Luongo, A. Sammartino, R. Giunta, A. Grassia, R. Ragone, and A. Tirelli. 1995. Hyperbaric oxygen, oxygen-ozone therapy, and rheologic parameters of blood in patients with peripheral occlusive arterial disease. Undersea Hyperb. Med. 22:17–22.

    PubMed  CAS  Google Scholar 

  18. Young, C., and D. K. Bhalla. 1995. Effects of ozone on the epithelial and inflammatory responses in the airways: role of tumor necrosis factor. J. Toxicol. Environ. Health 46:329–342.

    PubMed  CAS  Google Scholar 

  19. Wielgus-Serafińska, E., A. Plewka, and M. Kamiński. 1993.Circadian variation of mitochondrial succinic dehydrogenase and microsomal cytochrome P-450 dependent monooxygenase activity in the liver of sexually immature and mature rats. J. Physiol. Pharmacol. 44:55–63.

    PubMed  Google Scholar 

  20. Czekaj, P., A. Plewka, M. Kamiński, G. Nowaczyk, K. Pawlicki, and E. Wielgus-Serafińska. 1994. Daily and circadian rhythms in the activity of mixed function oxidases system in rats of different age. Biol. Rhythm. Res. 25:67–75.

    CAS  Google Scholar 

  21. Misra, H. P., and I. Fridovich. 1972. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol. Chem. 247:3170–3175.

    PubMed  CAS  Google Scholar 

  22. Aebi, M. 1974. Catalase, methods of enzymatic analysis. In: H. Bermeyer ed., Academic Press, NY, pp. 674–678.

  23. Madej, J. A., C. Kaszubkiewicz, M. Mazurkiewicz, S. Klientowski, and L. Fiszer. 1983. Test of usage chemiluminescence in diagnosis of lymphatic leukaemia P388 in mice. Med. Wet. 39:682–685.

    Google Scholar 

  24. Niwa, Y. 1989. Lipid peroxides and superoxide dismutase (SOD) induction in skin inflammatory diseases, and treatment with SOD preparations. Dermatologica 179(Suppl 1):101–106.

    Article  PubMed  Google Scholar 

  25. Iizawa, O., T. Kato, H. Tagami, and Y. Niwa. 1991. Lipid peroxides and superoxide dismutase activity in trichophyte lesions. Arch. Dermatol. 127:1241–1243.

    Article  PubMed  CAS  Google Scholar 

  26. Gregory, E. M., S. A. Goscin, and I. Fridovich. 1974. Superoxide dismutase and oxygen toxicity in a eukaryote. J. Bacteriol. 117:456–460.

    PubMed  CAS  Google Scholar 

  27. Archibald, F. 2003. Oxygen toxicity and the health and survival of eukaryote cells: a new piece is added to the puzzle. Proc. Natl. Acad. Sci. U.S.A. 100:10141–10143.

    Article  PubMed  CAS  Google Scholar 

  28. Aruoma, O. I., B. Halliwell, and M. Dizdaroglu. 1989. Iron ion-dependent modification of bases in DNA by the superoxide radical-generating system hypoxanthine/xanthine oxidase. J. Biol. Chem. 264:13024–13028.

    PubMed  CAS  Google Scholar 

  29. Takehara, Y., K. Yamaoka, E. F. Sato, T. Yoshioka, and K. Utsumi. 1994. DNA damage by various forms of active oxygens and its inhibition by different scavengers using plasmid DNA. Physiol. Chem. Phys. Med. NMR 26:215–226.

    PubMed  CAS  Google Scholar 

  30. Wolff, S. P., and R. T. Dean. 1986. Fragmentation of proteins by free radicals and its effect on their susceptibility to enzymic hydrolysis. Biochem. J. 234:399–403.

    PubMed  CAS  Google Scholar 

  31. Davies, K. J. 1987. Protein damage and degradation by oxygen radicals. I. general aspects. J. Biol. Chem. 262:9895–9901.

    PubMed  CAS  Google Scholar 

  32. Halliwell, B., and J. M. Gutteridge. 1988. Free radicals and antioxidant protection: mechanisms and significance in toxicology and disease. Human Toxicol. 7:7–13.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department and Clinic of Gynaecological Endocrinology, Medical University of Silesia, ul. Medyków 14, 40-752, Katowice, Poland

    Pawel Madej & Grzegorz Franik

  2. Department of Protein Chemistry and Enzymology, Medical University of Silesia, ul. Narcyzów 1, 41-200, Sosnowiec, Poland

    Andrzej Plewka

  3. Department of Pathological Anatomy and Forensic Medicine, Veterinary Academy, ul. Norwida 31, 50-375, Wroclaw, Poland

    Janusz A. Madej & Marcin Nowak

  4. Department of Histology and Embryology, Medical University of Silesia, ul. Medyków 18, 40-752, Katowice, Poland

    Danuta Plewka

  5. Department of Pathomorphology, Medical University of Silesia, ul. Medyków 18, 40-752, Katowice, Poland

    Darek Golka

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  1. Pawel Madej
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  2. Andrzej Plewka
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  3. Janusz A. Madej
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Correspondence to Pawel Madej.

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Madej, P., Plewka, A., Madej, J.A. et al. Ozonotherapy in an Induced Septic Shock. I. Effect of Ozonotherapy on Rat Organs in Evaluation of Free Radical Reactions and Selected Enzymatic Systems. Inflammation 30, 52–58 (2007). https://doi.org/10.1007/s10753-007-9021-7

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  • DOI: https://doi.org/10.1007/s10753-007-9021-7

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