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Growth rates and tolerance to low water temperatures of freshwater bacterioplankton strains: ecological insights from shallow hypereutrophic lakes in Japan

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Abstract

To investigate differences in culturable freshwater bacterioplankton between summer and winter, we examined specific bacterioplankton in the low temperature surface water of two shallow, hypereutrophic, temperate lakes in Japan over two winters, and compared our results with the previously published data. We used the size-exclusion assay method (SEAM), a cultivation-based approach that simply and effectively isolates typical freshwater bacterioplankton. The specific clusters detected in the winter samples were the Rhodoferax sp. BAL47 cluster (LimA and LimC), a predominant and ubiquitous freshwater lineage, and the LiUU-5-340 cluster. To confirm tolerance to low water temperature of winter-specific groups, we also compared growth rates at 5°C among several pure strains of typical freshwater bacterioplankton clusters belonging to the Rhodoferax sp. BAL47, Polynucleobacter necessarius, GKS98, LiUU-5-340, and IRD18C08 of the class Betaproteobacteria, and the Luna-1 and Luna-2 of the class Actinobacteria. Specific detectability of freshwater bacterioplankton clusters/subclusters by SEAM in the winter sample substantially correlated with the low temperature-specific growth characteristics of each isolate. Response to water temperature is a key control factor in freshwater bacterioplankton assemblage composition. These results provide important insights into the specific response to water temperature of several ubiquitous culturable freshwater bacterioplankton clusters inhabiting a temperate climate zone.

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Acknowledgements

We thank T. Iida for helping in depositing the isolated strains in the Japan Collection of Microorganisms. We acknowledge the members of Ibaraki Kasumigaura Environmental Science Center for their kind cooperation. This work was supported by a Grant-in-Aid for Young Scientists (B) 23710023 and 15K16122 from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Center for Environmental Science in Saitama, 914 Kamitanadare, Kazo, Saitama, 347-0115, Japan

    Keiji Watanabe

  2. Tokyo Metropolitan Research Institute for Environmental Protection, 1-7-5 Shinsuna, Koto-ku, Tokyo, 136-0075, Japan

    Yuichi Ishii

  3. Marine Biological Research Institute of Japan Co., Ltd, 4-3-16 Yutaka-cho, Shinagawa-ku, Tokyo, 142-0042, Japan

    Nobuyuki Komatsu

  4. Ibaraki Kasumigaura Environmental Science Center, 1853 Okijuku, Tsuchiura, Ibaraki, 300-0023, Japan

    Tatsumi Kitamura

  5. National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki, 305-8506, Japan

    Mirai Watanabe, Shigeki Yamamura, Akio Imai & Seiji Hayashi

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  1. Keiji Watanabe
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  2. Yuichi Ishii
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  3. Nobuyuki Komatsu
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  4. Tatsumi Kitamura
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  5. Mirai Watanabe
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  8. Seiji Hayashi
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Correspondence to Keiji Watanabe.

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Watanabe, K., Ishii, Y., Komatsu, N. et al. Growth rates and tolerance to low water temperatures of freshwater bacterioplankton strains: ecological insights from shallow hypereutrophic lakes in Japan. Hydrobiologia 792, 67–81 (2017). https://doi.org/10.1007/s10750-016-3045-7

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  • DOI: https://doi.org/10.1007/s10750-016-3045-7

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