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The challenging role of life cycle monitoring: evidence from bisphenol A on the copepod Tigriopus japonicus

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Abstract

We used the marine model copepod taxon T. japonicus Mori, 1938 for a first short sublethal life cycle assay of the endocrine-disrupting chemical bisphenol A (BphA) where we studied life cycle and reproductive success. Individual copepodid-I-stages were reared in 96-well microplates to adulthood in as short as 6 days in only 200 μl of 0.1 mg bisphenol (BphA/L) or seawater solution as a control (CON). Males and females were then mated in one well and checked daily for the following endpoints: postmating days to female brood sac extrusion, days from brood sac extrusion to naupliar hatching, first clutch brood size, hatching success, and reproductive success. Mean naupliar hatching times ranged from 1.3 days for CON-reared mating pairs to 2.7 days for BphA-reared mating pairs; mean brood size was significantly lower for CON females mated with BphA males than for all other mating combinations; reproductive success of CON females mated with BphA males and BphA females mated with BphA males showed significant differences between females and males. In conclusion, T. japonicus complete life cycle exposures to BphA at an environmentally realistic concentration resulted generally in reproductive depression with effect values depending on the exposure history of the different gender.

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Acknowledgements

This research was supported by “Long-Term Change of Structure and Function in Marine Ecosystems of Korea” and “Development of Korea Operational Oceanographic System (KOOS)” funded by the Ministry of Oceans and Fisheries, Korea.” H.U.D further acknowledges the funding by MOST 104-2621-M-037-001 to T.H. SHIH. This work was partly supported by the grant from Research Center for Environmental Medicine, Kaohsiung Medical University (KMU-TP105A27) to H.U.D. We also thank the Asia-Pacific Ocean Research Center of the Department of Oceanography [No. 76211194] in the frame of KMU/NSYSU cooperation to H.U.D. and K.S. We are grateful for useful comments on an earlier draft of the MS by Profs. J.S. Lee and J.S. Rhee.

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Authors and Affiliations

  1. Department of Biomedical Science and Environmental Biology, KMU - Kaohsiung Medical University, No. 100, Shih-Chuan 1st Road, Kaohsiung, 80708, Taiwan, ROC

    Hans-Uwe Dahms & Wei-Yu Chen

  2. Department of Marine Biotechnology and Resources, National Sun Yat-sen University, No. 70, Lienhai Road, Kaohsiung, 80424, Taiwan, ROC

    Hans-Uwe Dahms

  3. Department of Environmental Resources and Management, Chia Nan University of Pharmacy & Science, Tainan, Taiwan, ROC

    Da-Ji Huang

  4. Department of Oceanography, Pusan National University, Busan, 609-735, Korea

    Sang Heon Lee

  5. Institute of Marine Biology, National Taiwan Ocean University, Keelung, Taiwan, ROC

    Jiang-Shiou Hwang

Authors
  1. Hans-Uwe Dahms
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  2. Sang Heon Lee
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  3. Da-Ji Huang
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  4. Wei-Yu Chen
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  5. Jiang-Shiou Hwang
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Correspondence to Jiang-Shiou Hwang.

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Handling editor: Juan Carlos Molinero

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Dahms, HU., Lee, S.H., Huang, DJ. et al. The challenging role of life cycle monitoring: evidence from bisphenol A on the copepod Tigriopus japonicus . Hydrobiologia 784, 81–91 (2017). https://doi.org/10.1007/s10750-016-2859-7

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  • DOI: https://doi.org/10.1007/s10750-016-2859-7

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