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Morphological, biochemical and molecular characterization of Anabaena, Aphanizomenon and Nostoc strains (Cyanobacteria, Nostocales) isolated from Portuguese freshwater habitats

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Abstract

Studies of cyanobacterial nostocacean taxa are important to the global scientific community, mainly because a significant number of beneficial strains that belong to the order Nostocales fix atmospheric nitrogen, thus contributing to the fertility of agricultural soils worldwide, while others behave as nuisance microorganisms in aquatic ecosystems due to their involvement in toxic bloom events. However, in spite of their ecological importance and environmental concerns, their identification and taxonomy are still problematic and doubtful, often being based on current morphological and physiological studies, which generate confusing classification systems and usually vary under different conditions. Therefore, the present research aimed to investigate through a polyphasic approach differences in morphological, biochemical and genotypic features of three nostocacean cyanobacterial strains isolated from central-western Portuguese shallow freshwater bodies. Morphometric, genetic (16S rRNA, nifH and hetR fragments) and biochemical (fatty acid methyl ester; FAME profiles) data were used to characterize the strains. Morphological analysis and sequencing of 16S rRNA fragments showed that the strains belonged to Anabaena cylindrica (UTAD_A212), Aphanizomenon gracile (UADFA16) and Nostoc muscorum (UTAD_N213) species. These strains showed clear distinct morphological and genetic features, allowing easy allocation to their respective genera. The same happened by using partial sequences of hetR and nifH genes, in spite of the scarcity of deposited sequences. Biochemical characterization showed that the FAME profiles obtained were consistent with both morphological and molecular analyses. It was suggested that the ratio of monounsaturated to polyunsaturated FAMEs, together with the unsaturation index, could be used as genus-specific chemotaxonomic biomarkers.

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Acknowledgments

This study was supported by PhD grants to Victor Galhano (SFRH/BD/17582/2004) and Daniela de Figueiredo (SFRH/BD/23864/2005) of Portuguese Science and Technology Foundation (FCT), Ministry of Science, Technology and Higher Education, Portuguese Government. The authors are indebted to Prof. Fernando Nunes (Chemistry Department, UTAD, Vila Real) for instrumental laboratory facilities on lipid analysis and to Dr. Fátima Santos (Botany Department, FCTUC, Coimbra) for her kind help on morphological identification and loan of precious historical publications. Further, we are thankful to Prof. Luigi Naselli-Flores (Department of Botanical Sciences, University of Palermo, Palermo, Italy) and to the anonymous reviewer for their constructive and helpful comments on the manuscript.

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  1. Department of Biology and Environment, CITAB—Centre for Research and Technology of Agro-Environment and Biological Sciences, Sustainable Agro-Food Chains Research Group, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal

    Victor Galhano & José Gomes-Laranjo

  2. Department of Biology, CESAM—Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal

    Daniela R. de Figueiredo, Artur Alves, António Correia & Mário J. Pereira

  3. Department of Chemistry, CECAV—Centre of Animal Sciences and Veterinary, University of Trás-os-Montes and Alto Douro, Apartado 1013, 5001-801, Vila Real, Portugal

    Francisco Peixoto

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Galhano, V., de Figueiredo, D.R., Alves, A. et al. Morphological, biochemical and molecular characterization of Anabaena, Aphanizomenon and Nostoc strains (Cyanobacteria, Nostocales) isolated from Portuguese freshwater habitats. Hydrobiologia 663, 187–203 (2011). https://doi.org/10.1007/s10750-010-0572-5

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