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Population Genetic Structure of Astyanax scabripinnis (Teleostei, Characidae) from an Urban Stream

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Abstract

Despite the great anthropogenic interference on urban streams, information is still scarce about the genetic variability and structure of native fish populations inhabiting such streams. In the present study, random amplified polymorphic DNA (RAPD) markers were used to analyze genetic variability and structure of populations assigned to the Neotropical fish species Astyanax scabripinnis from an urban stream located in Londrina, Paraná State, southern Brazil. Thirty individuals of this species were collected from three sites throughout the upper Cambé stream. A total of 10 primers amplified 159 loci, of which 128 (80.5%) were polymorphic. Each of the three populations showed very similar proportions of polymorphic loci, which ranged from 63.5 to 64.8%. Unbiased genetic distances varied from 0.0612 to 0.0646. Thetap-test values indicated moderate to high genetic differentiation among individuals from different localities. The number of migrants varied from 1.34 to 1.46, suggesting a low gene flow between populations. The genetic similarity among all individuals studied ranged from 0.424 to 0.848. The results suggest that populations of A. scabripinnis in Cambé stream are undergoing genetic differentiation.

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References

  • F. S. Almeida M. H. P. Fungaro L. M. K. Sodré (2001) ArticleTitleRAPD and isoenzyme analysis of genetic variability in three allied species of catfish (Siluriformes: Pimelodidae) from the Tibagi river, Brazil Journal of Zoology 253 113–120

    Google Scholar 

  • APHA, 1998. American Public Health Association. Standard methods for the examination of water and waste-water, 20th edn. Washington, USA.

  • G. Barbieri (1992a) ArticleTitleBiologia de Astyanax scabripinnis paranae (Characiformes, Characidae) do ribeirão do Fazzari. São Carlos. Estado de São Paulo. I. Estrutura populacional e crescimento Revista Brasileira de Biologia 52 579–586

    Google Scholar 

  • G. Barbieri (1992b) ArticleTitleBiologia de Astyanax scabripinnis paranae (Characiformes, Characidae) do ribeirão do Fazzari. São Carlos. Estado de São Paulo. II. Aspectos quantitativos da reprodução Revista Brasileira de Biologia 52 589–596

    Google Scholar 

  • J. W. Bickham S. Sandhu P. D. N. Hebert L. Chickhi R. Athwal (2000) ArticleTitleEffects of chemical contaminants on genetic diversity in natural populations: implications for biomonitoring and ecotoxicology Mutation Research 463 33–51 Occurrence Handle10838208 Occurrence Handle1:CAS:528:DC%2BD3cXjs1Cntbo%3D

    PubMed  CAS  Google Scholar 

  • Britski, H. A., 1972. Peixes de água doce do estado de São Paulo: Sistemática. In: Comissão Inter Estadual da Bacia Paraná-Uruguai, Poluição e Piscicultura. Faculdade de Saúde Pública da USP, Instituto de Pesca, São Paulo: 79–108.

  • G. R. Carvalho (1993) ArticleTitleEvolutionary aspects of fish distribution: genetic variability and adaptation Journal of Fish Biology 43 53–73 Occurrence Handle10.1111/j.1095-8649.1993.tb01179.x

    Article  Google Scholar 

  • InstitutionalAuthorNameCETESB (2001) Companhia de tecnologia de saneamento ambiental. Relatório de qualidade das águas interiores do estado de São Paulo, ano de 2000 Série Relatórios/CETESB São Paulo, Brazil

    Google Scholar 

  • L. Chiari L. M. K. Sodré (2001) ArticleTitleStudy of eight species of Anostomidae family (Pisces, Characiformes) by RAPD analysis Acta Scientiarum 23 445–451

    Google Scholar 

  • R. Cimmaruta F. Scialanca F. Luccioli G. Nascetti (2003) ArticleTitleGenetic diversity and environmental stress in Italian populations of the cyprinodont fish Aphanius fasciatus Oceanologica Acta 26 101–110 Occurrence Handle10.1016/S0399-1784(02)01234-3

    Article  Google Scholar 

  • A. S. G. Coelho (2000) BOOD: avaliação de dendrogramas baseados em estimativas de distâncias/similaridades genéticas através do procedimento de bootstrap. Software UFG, Goiânia Goiás

    Google Scholar 

  • A. S. Foré S. I. Guttman A. J. Bailer D. J. Altfater B. V. Counts (1995a) ArticleTitleExploratory analysis of population genetic assessment as a water quality indicator. I. Pimephales notatus Ecotoxicology and Environmental Safety 30 24–35

    Google Scholar 

  • A. S. Foré S. I. Guttman A. J. Bailer D. J. Altfater B. V. Counts (1995b) ArticleTitleExploratory analysis of population genetic assessment as a water quality indicator. II. Campostoma anomalum Ecotoxicology and Environmental Safety 30 36–46

    Google Scholar 

  • V. Garutti H. A. Britski (2000) ArticleTitleDescrição de uma espécie nova de Astyanax (Teleostei: Characidae) na bacia do alto rio Paraná e considerações sobre as demais espécies do gênero na bacia. Comunicações do Museu de Ciências e Tecnologia da PUCRS Sér. Zool. Porto Alegre 13 65–88

    Google Scholar 

  • Guttman, S. I. & D. Berg, 1998. Changes in the genetic diversity of aquatic organisms in the great lakes: causes and consequences. Setac News : 23–24.

  • S. M. Haig (1998) ArticleTitleMolecular contributions to conservation Ecology 79 413–425

    Google Scholar 

  • D. L. Hartl A. G. Clark (1997) Principles of Population Genetics EditionNumber3 Sinauer Associates, Inc Sunderland, MA

    Google Scholar 

  • N. Isabel J. Beaulieu P. Thériault J. Bousquet (1999) ArticleTitleDirect evidence for biased gene diversity estimates from dominant random amplified polymorphic DNA (RAPD) fingerprints Molecular Ecology 8 477–483 Occurrence Handle10.1046/j.1365-294X.1999.00597.x

    Article  Google Scholar 

  • G. F. Landini A. R. Schwantes M. L. B. Schwantes (2002) ArticleTitle Astyanax scabripinnis (Pisces: Characidae) hemoglobins: structure and function Brazilian Journal of Biology 62 595–599 Occurrence Handle10.1590/S1519-69842002000400006 Occurrence Handle1:STN:280:DC%2BD3s7ksValsg%3D%3D

    Article  CAS  Google Scholar 

  • N. G. Lemos A. L. Dias A. T. Silva-Souza M. S. Mantovani (2005) ArticleTitleEvaluation of environmental waters using the comet assays in Tilapia rendalli Enviromental Toxicology and Pharmacology 19 197–201 Occurrence Handle1:CAS:528:DC%2BD2cXhtVKrtLnJ

    CAS  Google Scholar 

  • M. S. P. Leuzzi F. S. Almeida M. L. Orsi L. M. K. Sodré (2004) ArticleTitleAnalysis by RAPD of the genetic structure of Astyanax altiparanae (Pisces, Characiformes) in reservoirs on the Paranapanema river Brazil Genetics and Molecular Biology 27 355–362 Occurrence Handle10.1590/S1415-47572004000300009 Occurrence Handle1:CAS:528:DC%2BD2cXps1yntbk%3D

    Article  CAS  Google Scholar 

  • M. Lynch B. G. Milligan (1994) ArticleTitleAnalysis of population structure with RAPD markers Molecular Ecology 3 91–99 Occurrence Handle8019690 Occurrence Handle1:STN:280:DyaK2c3ptVKltw%3D%3D

    PubMed  CAS  Google Scholar 

  • J. V. MacArthur L. G. Leff M. H. Smith (1992) ArticleTitleGenetic diversity of bacteria along a stream continuum Journal of the North American Benthological Society 11 269–277

    Google Scholar 

  • E. L. Maistro C. Oliveira F. Foresti (2000) ArticleTitleSympatric occurrence of two cytotypes of Astyanax scabripinnis (Characiformes, Characidae) Genetics and Molecular Biology 23 365–369 Occurrence Handle10.1590/S1415-47572000000200021

    Article  Google Scholar 

  • D. A. Matoso R. F. Artoni P. M. Galetti Jr. (2004) ArticleTitleGenetic diversity of the small characid fish Astyanax sp., and its significance for conservation Hydrobiologia 527 223–225 Occurrence Handle10.1023/B:HYDR.0000043303.02986.71

    Article  Google Scholar 

  • Miller M. P., 1997. Tools for population genetic analyses (TFPGA) 1.3: a Windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author.

  • O. Moreira-Filho L. A. C. Bertollo (1991) ArticleTitle Astyanax scabripinnis (Pisces, Characidae): a species complex Revista Brasileira de Genética 14 331–357

    Google Scholar 

  • C. B. Moysés L. F. Almeida-Toledo (2002) ArticleTitleRestriction fragments length polymorphisms of mitochondrial DNA among five freshwater fish species of the genus Astyanax (Pisces, Characidae) Genetics and Molecular Biology 25 401–408

    Google Scholar 

  • M. H. Murdoch P. D. N. Hebert (1994) ArticleTitleMitochondrial DNA diversity of brown bullhead from contaminated and relatively pristine sites in the Great Lakes Environmental Toxicology and Chemistry 13 1281–1289 Occurrence Handle1:CAS:528:DyaK2cXlsVWjsL8%3D

    CAS  Google Scholar 

  • S. Nadig K. L. Lee S. M. Adams (1998) ArticleTitleEvaluation of alterations of genetic diversity in sunfish populations exposed to contaminants using RAPD assay Aquatic Toxicology 43 163–178 Occurrence Handle10.1016/S0166-445X(98)00049-6 Occurrence Handle1:CAS:528:DyaK1cXmvFWisLY%3D

    Article  CAS  Google Scholar 

  • M. Nei (1978) ArticleTitleEstimation of average heterozygosity and genetic distance from a small number of individuals Genetics 89 583–590 Occurrence Handle17248844

    PubMed  Google Scholar 

  • T. Olsen L. Ellerbeck T. Fisher A. Callaghan M. Crane (2001) ArticleTitleVariability in acetylcholinesterase and glutathione S-transferase activities in Chironomus riparus meigen deployed in situ at uncontaminated field sites Environmental Toxicology and Chemistry 20 1725–1732 Occurrence Handle10.1897/1551-5028(2001)020<1725:VIAAGS>2.0.CO;2 Occurrence Handle11491555 Occurrence Handle1:CAS:528:DC%2BD3MXlsVKqurY%3D

    Article  PubMed  CAS  Google Scholar 

  • M. J. Paul J. L. Meyer (2001) ArticleTitleStreams in the urban landscape Annual Review of Ecology and Systematics 32 335–365 Occurrence Handle10.1146/annurev.ecolsys.32.081501.114040

    Article  Google Scholar 

  • S. M. A. P. Prioli A. J. Prioli H. F. Júlio C. S. Pavanelli A. V. Oliveira H. Carrer D. M. Carraro L. M. Prioli (2002) ArticleTitleIdentification of Astyanax altiparanae (Teleostei, Characidae) in the Iguaçu River, Brazil, based on mitochondrial DNA and RAPD markers Genetics and Molecular Biology 25 421–430 Occurrence Handle10.1590/S1415-47572002000400011 Occurrence Handle1:CAS:528:DC%2BD3sXit1ylsLw%3D

    Article  CAS  Google Scholar 

  • Rohlf F. J., 1992. NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Applied Biostatistics, Setauket, New York.

  • N. Ryman F. Utter L. Laikre (1995) ArticleTitleProtection of intraspecific biodiversity of exploited fishes Reviews in Fish Biology and Fisheries 5 417–446 Occurrence Handle10.1007/BF01103814

    Article  Google Scholar 

  • M. Slatkin (1993) ArticleTitleIsolation by distance in equilibrium and non-equilibrium populations Evolution 47 264–279

    Google Scholar 

  • A. J. Solé-Cava (2001) Biodiversidade molecular e genética da conservação S. R. Matioli (Eds) Biologia Molecular e Evolução EditionNumber(1st edn). Holos Ribeirão Preto 171–190

    Google Scholar 

  • I. L. Souza O. Moreira-Filho (1995) ArticleTitleCytogenetic diversity in the Astyanax scabripinnis (Pisces, Characidae) complex I. Allopatric distribution in a small stream Cytologia 60 1–11

    Google Scholar 

  • I. L. Souza O. Moreira-Filho A. C. Bertollo (1995) ArticleTitleCytogenetic diversity in the Astyanax scabripinnis (Pisces, Characidae) complex II. Different cytotypes living in sympatry Cytologia 60 273–281

    Google Scholar 

  • C. W. Theodorakis L. R. Shuggart (1997) ArticleTitleGenetic ecotoxicology: II. Population genetic structure in mosquitofish exposed in situ to radionuclides Ecotoxicology 6 335–354 Occurrence Handle1:CAS:528:DyaK2sXnvFOns7s%3D

    CAS  Google Scholar 

  • R. L. Tremblay J. D. Ackerman (2001) ArticleTitleGene flow and effective population size in Leopanthes (Orchidaceae): a case for genetic drift Biological Journal of the Linnean Society 72 47–62 Occurrence Handle10.1006/bijl.2000.0485

    Article  Google Scholar 

  • A. M. Veregue M. L. Orsi (2003) ArticleTitleBiologia reprodutiva de Astyanax scabripinnis paranae (Eigenmann) (Ostheichthyes, Characidae), do ribeirão das Marrecas, bacia do rio Tibagi, Paraná Revista Brasileira de Zoologia 20 97–105 Occurrence Handle10.1590/S0101-81752003000100012

    Article  Google Scholar 

  • A. P. Wasco P. M. Galetti SuffixJr. (2002) ArticleTitleRAPD analysis in the Neotropical fish Brycon lundii: genetic diversity and its implications for the conservation of the species Hydrobiologia 474 131–137

    Google Scholar 

  • B. S. Weir C. C. Cockerham (1984) ArticleTitleEstimating F-statistics for the analysis of population structure Evolution 38 1358–1370

    Google Scholar 

  • A. Widmer P. Schmid-Hempel (1999) ArticleTitleThe population genetic structure of a large temperate pollinator species, Bombus pascuorum (Scopoli) (Hymenoptera: Apidae) Molecular Ecology 8 387–398 Occurrence Handle10199006

    PubMed  Google Scholar 

  • J. G. K. Williams A. R. Kubelik K. L. Livak S. V. Tingey (1990) ArticleTitleDNA polymorphism amplified by arbitrary primers are useful as genetic markers Nucleic Acids Research 18 6531–6535 Occurrence Handle1979162 Occurrence Handle1:CAS:528:DyaK3MXjslWmsA%3D%3D

    PubMed  CAS  Google Scholar 

  • E. U. Winkaler A. G. Silva H. C. Galindo C. B. R. Martinez (2001) ArticleTitleBiomarcadores histológicos e fisiológicos para o monitoramento da saúde de peixes de ribeirões de Londrina Estado do Paraná Acta Scientiarum 23 507–514

    Google Scholar 

  • S. Wright (1978) Evolution and Genetics of Populations University of Chicago Press Chicago

    Google Scholar 

  • M. J. S. Yabe E. O. Oliveira (1998) ArticleTitleMetais pesados em águas superficiais como estratégia de caracterização de bacias hidrográficas Química Nova 21 551–556 Occurrence Handle1:CAS:528:DyaK1cXmt1ClsL4%3D

    CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratório de Marcadores Moleculares em Peixes e Ecologia de Abelhas, Departamento de Biologia Geral, Universidade Estadual de Londrina, Campus Universitário, 86051-990, Londrina, PR, Brazil

    Silvia H. Sofia, Carlos R.M. Silva, Bruno A. Galindo, Fernanda S. Almeida & Leda M.K. Sodré

  2. Laboratório de Ecofisiologia Animal, Departamento de Ciências Fisiológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil

    Cláudia B.R. Martinez

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  1. Silvia H. Sofia
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  2. Carlos R.M. Silva
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  3. Bruno A. Galindo
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  4. Fernanda S. Almeida
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  5. Leda M.K. Sodré
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  6. Cláudia B.R. Martinez
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Correspondence to Silvia H. Sofia.

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Sofia, S.H., Silva, C.R., Galindo, B.A. et al. Population Genetic Structure of Astyanax scabripinnis (Teleostei, Characidae) from an Urban Stream. Hydrobiologia 553, 245–254 (2006). https://doi.org/10.1007/s10750-005-1106-4

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