Abstract
Retinoblastoma protein (pRb) phosphorylation plays a central role in mediating cell cycle G1/S stage transition, together with E2F transcription factors. The binding of pRb to E2F is thought to be controlled by the sequential and cumulative phosphorylation of pRb at various amino acids. In addition to well characterized roles as a tumor suppressor, pRb has more recently been implicated in osteoprogenitor and other types of stem cell maintenance, proliferation and differentiation, thereby influencing the morphogenesis of developing organs. In this study, we present data characterizing the expression of pRb and three phosphorylated pRb (ppRb) isoforms—ppRbS780, ppRbS795, ppRbS807/811—in developmentally staged mouse molar and incisor teeth. Our results reveal distinct developmental expression patterns for individual ppRb isoforms in dental epithelial and dental mesenchymal cell differentiation, suggesting discrete functions in tooth development.
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Acknowledgments
The authors would like to thank the Yelick and Hinds Laboratory members for support and expert advice.
Conflict of interest
We acknowledge that there are no perceived or actual conflicts of interest to disclose, and support from ARRA funded NIH/NIDCR grant DE016962 (PCY).
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Zhang, W., Vazquez, B., Andreeva, V. et al. Discrete phosphorylated retinoblastoma protein isoform expression in mouse tooth development. J Mol Hist 43, 281–288 (2012). https://doi.org/10.1007/s10735-012-9404-4
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DOI: https://doi.org/10.1007/s10735-012-9404-4