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Novel microRNAs regulating ripening-associated processes in banana fruit

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Abstract

MicroRNAs (miRNAs) modulate gene expression and regulate various physiological and developmental processes in plants. During fruit ripening phase, several physiological and biochemical variations take place resulting changes in colour and texture, softening and production of aroma volatiles. A number of pathways leading to cell wall hydrolysis, ethylene signaling, biosynthesis of fatty acids, esters and a number of secondary plant products play important role during fruit ripening. However, detailed analysis of various processes regulated by miRNAs has not been studied in detailed in a number of fruits. In this study, we sequenced small RNA libraries from ripe and un-ripe fruit of Banana (Musa acuminata), an important and staple food crop, to identify miRNAs regulating fruit ripening. Our analysis identified a number of novel miRNAs which are differentially expressed during fruit ripening. These novel miRNAs were analyzed for their precursors, chromosome localization and targets. Some of these miRNAs were identified to target genes involved in miRNA biogenesis, fruit softening and aroma biosynthesis. This study advances our knowledges in the area of fruit ripening process regulated by miRNAs.

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Acknowledgements

This work was supported by the Department of Biotechnology, New Delhi under NER-Banana Programme and Council of Scientific and Industrial Research, New Delhi (Network Project-BSC 107).

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  1. Ashutosh Pandey & Deepika Sharma

    Present address: National Institute of Plant Genome Research, Aruna Asaf Ali Marg, P.O. Box No. 10531, New Delhi, 110 067, India

  2. Deepika Lakhwani and Sanchita have contributed equally to this work.

Authors and Affiliations

  1. CSIR-National Botanical Research Institute, Council of Scientific and Industrial Research (CSIR-NBRI), Rana Pratap Marg, Lucknow, 226001, India

    Deepika Lakhwani,  Sanchita, Ashutosh Pandey, Deepika Sharma, Mehar H. Asif & Prabodh K. Trivedi

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Deepika Lakhwani, Mehar H. Asif & Prabodh K. Trivedi

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  1. Deepika Lakhwani
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  2. Sanchita
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  3. Ashutosh Pandey
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Correspondence to Mehar H. Asif or Prabodh K. Trivedi.

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Lakhwani, D., Sanchita, Pandey, A. et al. Novel microRNAs regulating ripening-associated processes in banana fruit. Plant Growth Regul 90, 223–235 (2020). https://doi.org/10.1007/s10725-020-00572-w

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