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Sugars, antioxidant enzymes and IAA mediate salicylic acid to prevent rice spikelet degeneration caused by heat stress

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Abstract

Salicylic acid (SA) can alleviate damage to rice plants induced by heat stress, but its role in preventing spikelet degeneration under high-temperature stress has not been documented. Rice plants pretreated with SA (0–50 mmol L−1) were subjected to heat stress at 40 °C at the pollen mother cell meiosis stage for 10 days. The results indicated that there were no significant differences in grain yields and yield components among rice plants that were exogenously sprayed with SA (0–50 mmol L−1) under natural conditions. Under heat stress, the grain yield, spikelet number per panicle and setting rate in response to SA treatments were higher than under the control (0 mmol L−1 SA or NON-SA) treatment, especially with 1 and 10 mmol L−1 SA. A higher grain yield, spikelet number per panicle and setting rate were recorded in these two SA treatments compared with the NON-SA treatment. During this process, soluble sugars, proline, phytohormones including ABA, GA3, BRs, IAA, ZR and JA, and antioxidant enzymes, such as superoxide dismutase, peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), were induced by SA. Moreover, soluble sugars, IAA, POD and APX in the spikelets with SA treatments were not only higher than with the NON-SA treatment but the changing patterns were also similar to that of the spikelet number per panicle under natural conditions and heat stress. Therefore, our results suggest that sugars, antioxidant enzymes and IAA might mediate SA to prevent spikelet degeneration caused by heat stress.

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Acknowledgements

This work was funded by the National Natural Science Foundation of China (Grant No. 31501264 and 31561143003), the China National Rice Research Institute (Grant No. 2014RG004-4), the Special Fund for Agro-Scientific Research in The Public Interest (Grant No. 201203029), and the National System of Rice Industry (Grant No. CARS-01-27); National Food Science and Technology Project (2016 YFD 0300208).

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    1. State Key Laboratory of Rice Biology, China National Rice Research Institute, 359 Tiyuchang Road, Hangzhou, 310006, People’s Republic of China

      C. X. Zhang, B. H. Feng, T. T. Chen, X. F. Zhang, L. X. Tao & G. F. Fu

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    1. C. X. Zhang
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    2. B. H. Feng
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    Correspondence to L. X. Tao or G. F. Fu.

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    C. X. Zhang and B. H. Feng have contributed equally to this work.

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    Zhang, C.X., Feng, B.H., Chen, T.T. et al. Sugars, antioxidant enzymes and IAA mediate salicylic acid to prevent rice spikelet degeneration caused by heat stress. Plant Growth Regul 83, 313–323 (2017). https://doi.org/10.1007/s10725-017-0296-x

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