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The response of canola cultivars to lipo-chitooligosaccharide (Nod Bj V [C18:1, MeFuc]) and thuricin 17

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Abstract

Lipo-chitooligosaccharides (LCOs) are signal molecules that play a role in initiating the symbiotic legume-rhizobia and arbuscular mycorrhizal processes. Thuricin 17 is a bacteriocin produced by Bacillus thuringiensis strain NEB 17. Previous work has shown that the application of a 10−6 M LCO solution, or a 10−11 M thuricin 17 solution, can stimulate the germination and growth of a wide range of plants. The objectives were: to grow canola cultivar 04C111 under optimal temperature conditions in a greenhouse, and to assess the effect of LCO seed application and spray treatment on 04C111 floral initiation, architecture, and factors that contribute to yield; to assess the effect of a thuricin 17 seed application treatment upon factors that contribute to 04C111 yield in a stressfully high temperature greenhouse system; and to assess the effect of an LCO seed application and spray treatment on Topas floral initiation, architecture, and factors that contribute to yield, and seed quality, in a simulated “cool spring” greenhouse system. According to the 95 % confidence limits, Brassica napus cultivars grown from untreated seed produced 39 % fewer to 1 % fewer branches than plants grown from LCO-treated seed. Application of an LCO foliar spray increased the total of branch lengths per 04C111 plant by 16.93 ± 8.41 cm. Based on the stem-to-branch length ratio, LCO-sprayed 04C111 plants were 68 % less apically dominant than unsprayed plants. Topas plants that were sprayed with LCO produced seed with approximately 4.45 ± 0.23 % more oil (% d.w.) than unsprayed plants. For both cultivars, seed treatment with 10−6 M of LCO produced plants with a higher average sum of primary, secondary, and tertiary branch (≥1 cm) frequencies.

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Abbreviations

BIC:

Bayesian Information Criteria

cv:

Cultivar

RLK:

Chitin elicitor receptor kinase

DAP:

Days after plannting

GA:

Gibberellin

LCO:

Lipo-chitooligosaccharide (Nod Bj V [C181, MeFuc])

LysM:

Lysin motif

GlcNAc:

N-acetylglucosamine

PGR:

Plant growth regulator

PC:

Principal component

PCA:

Principal component analysis

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Acknowledgments

This work was completed thanks to funding acquired by Donald Smith from the Green Crop Network and the Eastern Canadian Oilseed Alliance.

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Authors and Affiliations

  1. Department of Plant Science, McGill University, Macdonald Campus, Raymond Building, Sainte-Anne-de-Bellevue, QC, H9X 3V9, Canada

    Timothy Schwinghamer, Alfred Souleimanov, Pierre Dutilleul & Donald L. Smith

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  1. Timothy Schwinghamer
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  2. Alfred Souleimanov
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  3. Pierre Dutilleul
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  4. Donald L. Smith
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Correspondence to Donald L. Smith.

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Schwinghamer, T., Souleimanov, A., Dutilleul, P. et al. The response of canola cultivars to lipo-chitooligosaccharide (Nod Bj V [C18:1, MeFuc]) and thuricin 17. Plant Growth Regul 78, 421–434 (2016). https://doi.org/10.1007/s10725-015-0104-4

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