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Exogenous trehalose differentially modulate antioxidant defense system in wheat callus during water deficit and subsequent recovery

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Abstract

To elucidate the resistance mechanism of exogenous trehalose on water deficit further, we investigated the effect of exogenous trehalose (50 mM) in wheat callus during water deficit and subsequent recovery. Enhanced levels of endogenous trehalose were detected in calli exposed to water deficit (W) and trehalose (T) medium, moreover, W plus T treatment showed an additive effect. Water deficit elevated the accumulation of ROS (hydrogen peroxide and formation rate of O .−2 ) and the endogenous MDA (Malonaldehyde), and resulted in the decrease of cell viability and biomass. Exogenous trehalose (TW) could alleviate the damage induced by water deficit, which was involved in the decrease of MDA and the generation of ROS, and resulted in elevating cell viability and biomass. Additionally, water deficit induced activity of antioxidative enzymes (Peroxidase, POD; Catalase, CAT; Glutathione reductase, GR). Content of AsA (Reduced ascorbate) was also increased by water deficit, while the content of GSH (Glutathione) showed the opposite effect. The combined effect of T and W treatment led to a higher activity of enzymatic antioxidants including SOD (Superoxide dismutase) and GR, and elevated the content of nonenzymatic antioxidants including AsA and GSH, but had a negative effect on enzymatic antioxidants including POD and CAT in comparison to the water deficit treatment alone. During recovery, calli treated by TW showed a greater reduction in ROS resulted in enhancing a higher cell viability and biomass. The scavenging mechanism of ROS by exogenous trehalose is mainly dependent on nonenzymatic antioxidants, especially AsA-GSH cycle, rather than enzymatic mechanisms and trehalose itself.

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Abbreviations

ROS:

Reactive oxygen species

H2O2 :

Hydrogen peroxide

O .−2 :

Superoxide anion radical

SOD:

Superoxide dismutase

POD:

Peroxidase

CAT:

Catalase

GR:

Glutathione reductase

AsA:

Reduced ascorbate

GHS:

Glutathione

MDA:

Malonaldehyde

PEG 6000:

Polyethylene glycol 6000

ABA:

Abscisic acid

TTC:

Triphenyltetrazolium chloride

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Acknowledgments

This research was supported by the Special Fund for Agro-scientific Research in the Public Interest of China (No. 200903007), Agronomy College of Henan Agricultural University, and the Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crop in Henan Province (Henan Agricultural University).

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  1. Ecology and Genetic Improvement of Food Crops in Henan Province, Henan Agricultural University, Zhengzhou, 450002, China

    Chao Ma, Zhiqiang Wang & Tongbao Lin

  2. College of Agricultural, Henan University of Science and Technology, Luoyang, 471003, China

    Beibei Kong

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Correspondence to Tongbao Lin.

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Ma, C., Wang, Z., Kong, B. et al. Exogenous trehalose differentially modulate antioxidant defense system in wheat callus during water deficit and subsequent recovery. Plant Growth Regul 70, 275–285 (2013). https://doi.org/10.1007/s10725-013-9799-2

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