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Exploration of the antioxidative defense system to characterize chickpea genotypes showing differential response towards water deficit conditions

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Abstract

The present investigation was carried out to characterize genotypic variability in chickpea for water deficit tolerance by exploring the antioxidative defense system and seedling growth. Twenty nine chickpea genotypes including cultivars and advanced lines were grown under control and water deficit conditions induced by adding 3 % mannitol. The genotypes showed differential response in seedling growth under water deficit conditions. The activities of catalase (CAT) and superoxide dismutase (SOD) were observed to be differentially expressed in the roots of various genotypes, under control and water deficit conditions. The contents of H2O2, malondialdehyde (MDA) and proline were also observed to be variable in the roots of all the genotypes, under control and water deficit conditions. Stress tolerance index for the various parameters, viz, CAT and SOD activity, H2O2, MDA and proline content, root length, shoot length and their biomass was determined and the level of stress resistance calculated. The genotypes which showed increased activities of CAT and SOD, decreased contents of H2O2 and MDA together with least affected seedling growth under water deficit conditions exhibited higher stress resistance capacity. Multivariate principal component analysis for all the parameters affected under water deficit conditions, grouped the genotypes into three clusters having different (high, moderate and low) levels of stress resistance. Complete linkage clustering grouped these genotypes into two major clusters-I and II. The genotypes present in sub–sub cluster ‘A1’ and sub cluster ‘B’ of major cluster-I have been observed to possess high stress resistance levels for respective parameters. It can thus be concluded that chickpea genotypes exhibiting increased stress resistance levels in relation to SOD and CAT activities, H2O2 and MDA contents and seedling growth would have higher stress tolerance under water deficit conditions.

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Acknowledgments

Thanks are due to Council of Scientific and Industrial Research, New Delhi for funding this project under the Emeritus Scientist scheme to Narinder Kaur.

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Authors and Affiliations

  1. Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India

    Kamaljit Kaur, Narinder Kaur & Anil K. Gupta

  2. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, 141004, India

    Inderjit Singh

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  1. Kamaljit Kaur
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  2. Narinder Kaur
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  3. Anil K. Gupta
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  4. Inderjit Singh
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Correspondence to Narinder Kaur.

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Kaur, K., Kaur, N., Gupta, A.K. et al. Exploration of the antioxidative defense system to characterize chickpea genotypes showing differential response towards water deficit conditions. Plant Growth Regul 70, 49–60 (2013). https://doi.org/10.1007/s10725-012-9777-0

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  • DOI: https://doi.org/10.1007/s10725-012-9777-0

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