Skip to main content
Log in

Rapid micropropagation system in vitro and antioxidant activity of Scabiosa tschiliensis Grunning

  • Brief communication
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

A rapid micropropagation system for Scabiosa tschiliensis Grunning, an ethnic medicinal plant, has been developed. Calluses were induced from leaf and petiole explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg l−1 thidiazuron and 0.5 mg l−1 2,4-dicholorophenoxyacetic acid. In this medium, callus induction rate was about 94.05 %. Adventitious shoots developed from leaf (86.30 %) and petiole (83.33 %) calluses when cultured on MS medium containing 4.0 or 2.0 mg l−1 N6-benzyladenine (BA), respectively. Up to 73.85 % of the regenerated shoots formed complete plantlets on MS medium supplemented with 2.0 mg l−1 indole-3-butyric acid, with an average of 3.25 roots per shoot. Quantitative analysis of flavonoids showed that the phytochemical profiles of calluses and regenerated plants were similar to that of wild-type plants. The 2, 2-diphenyl-1-picrylhydrazyl assay revealed that the flavonoid extracts of calluses, adventitious shoots and wild-type plants had stronger antioxidant activities, the inhibitory concentrations being 23.944, 31.329 and 26.502 μg ml−1, respectively, where 50 % of DPPH was scavenged (IC50). Results showed that this perennial herb could be used as a potential source of new natural antioxidants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Anonymous (1977) Chinese Traditional Medicine Dictionary. Shanghai People’s Public Health Publishing House, Shanghai

  • Danova K, Čellárová E, Macková A, Daxnerová Z, Kapchina-Toteva V (2010) In vitro culture of Hypericum rumeliacum Boiss. and production of phenolics and flavonoids. In Vitro Cell Dev Biol Plant 46:422–429

    Article  CAS  Google Scholar 

  • Dhandapani M, Kim DH, Hong SB (2008) Efficient plant regeneration via somatic embryogenesis and organogenesis from the explants of Catharanthus roseus. In Vitro Cell Dev Biol Plant 44:18–25

    CAS  Google Scholar 

  • Guo B, Gao M, Liu CZ (2007) In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir. Plant Cell Rep 26:261–265

    Article  PubMed  CAS  Google Scholar 

  • Hseu YC, Chen SC, Yechd YJ, Wang L, Yang HL (2008) Antioxidant activity of Antrodia camphorate on free radical-induced endothelial cell damage. J Ethnopharmacol 118:237–245

    Article  PubMed  Google Scholar 

  • Huo Q, Wang Y, Gao RY, Li WL, Du Y, Liu K (2010) Study on antioxidation for extraction of total flavonoid in Stellera chamaejsme L. in vitro. Anim Husband Feed Sci 31:83–84

    Google Scholar 

  • Kirby AJ, Schmidt RJ (1997) The antioxidant activity of Chinese herbs for eczema and of placebo herbs—I. J Ethnopharmacol 56:103–108

    Article  PubMed  CAS  Google Scholar 

  • Makunga NP, van Staden J (2008) An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L. Plant Cell Tissue Organ Cult 92:63–72

    Article  Google Scholar 

  • Mehta S, Rai PK, Rai DK, Rai NK, Rai AK, Bicanic D, Sharma B, Watal G (2010) LIBS-based detection of antioxidant elements in seeds of Emblica officinalis. Food Biophys 5:186–192

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Rai PK, Jaiswal D, Rai DK, Sharma B, Watal G (2010) Antioxidant potential of oral feeding of Cynodon dactylon extract on diabetes induced oxidative stress. J Food Biochem 34:78–92

    Article  CAS  Google Scholar 

  • Ruta C, Morone-Fortunato I (2010) In vitro propagation of Cistus clusii Dunal, an endangered plant in Italy. In Vitro Cell Dev Biol Plant 46:172–179

    Article  Google Scholar 

  • Sharma RK, Chatterji S, Rai DK, Mehta S, Rai PK, Singh RK, Watal G, Sharma B (2009) Antioxidant activities and phenolic contents of the aqueous extracts of some Indian medicinal plants. J Med Plants Res 3:944–948

    CAS  Google Scholar 

  • Wang JL, Wang Q, Wang J, Lu Y, Xiao X, Gong WZ, Liu JK (2009) Effect of different plant growth regulators on micro-tuber induction and plant regeneration of Pinellia ternate (Thunb) Briet. Physiol Mol Biol Plant 15:359–365

    Article  CAS  Google Scholar 

  • Wang JL, Wang J, Liu K, Xiao X, Gong WZ, Lu Y, Liu MF, Xu DT (2010) An efficient plant regeneration system with in vitro flavonoid accumulation for Hylotelephium tatarinowii (Maxim.) H. Ohba. In Vitro Cell Dev Biol Plant 46:445–450

    CAS  Google Scholar 

  • Zheng Q, Koike Kazuo, Han LK, Okuda H, Nikaido T (2004) New biologically active triterpenoid saponins from Scabiosa tschiliensis. J Nat Prod 67:604–613

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by National Basic Research Program of China (973 Program, 2009CB522300), Program of State Ethnic Affairs Commission (09ZY09), the “985” Project (MUC98504-14 and MUC98507-08), “111” Project (B08044) and the Fundamental Research Funds for the Central Universities (0910KYZY46).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Junli Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, J., Liu, K., Xu, D. et al. Rapid micropropagation system in vitro and antioxidant activity of Scabiosa tschiliensis Grunning. Plant Growth Regul 69, 305–310 (2013). https://doi.org/10.1007/s10725-012-9765-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-012-9765-4

Keywords

Navigation