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Identification of four Eucalyptus genes potentially involved in cell wall biosynthesis and evolutionarily related to SHINE transcription factors

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Abstract

Recently, a new Arabidopsis thaliana master regulator of plant cell wall biosynthesis was characterized. It was named SHINE transcription factor (SHINE TF). This work searched for homologous genes in Eucalyptus grandis genome draft. RNAseq data, phylogeny analysis and qRT-PCR experiments were performed to complement SHINE gene analysis. By similarity searches using A. thaliana SHINE genes, four sequences were identified in Eucalyptus. Two of them contain all conserved motifs and characteristic features of this family, being assumed as true SHINE TFs and named EgrSHN1 and EgrSHN2. The other two sequences contain an incomplete ‘mm’ motif and were not considered true SHINE TFs, being further referred as Egr33m and Egr40m. Expression analysis revealed that EgrSHN1 is more expressed in flowers than in leaves and immature xylem, and both EgrSHN1 and EgrSHN2 are absent from adult xylem RNAseq libraries. This expression profile is similar to A. thaliana orthologues. On the other hand, Egr33m and Egr40m expression was detected in adult xylems. The phylogenetic studies indicate that both EgrSHNs were originated by gene duplication events which, together with gene loss, are hypothesized as common events in SHINE evolution. In conclusion, it is possible that the overexpression of SHINE genes in Eucalyptus xylem can generate information about wood formation processes, allowing an effective increase in forest plantation productivity.

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Acknowledgments

This work was supported by International Paper do Brazil Ltda., which offered financial contribution and supplied this research with plant materials. The authors wish to thank Professor Andy Pereira, Ph.D (University of Arkansas; Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University) due to his contribution in experimental design; Dr. Jorge M. Mondego (Centro de Pesquisa e Desenvolvimento em Recursos Genéticos Vegetais, Instituto Agronômico de Campinas) and Dr. Ana C. Deckmann (Faculdade de Engenharia Química, Universidade Estadual de Campinas) for their valuable collaboration in the revision of this work. The four EgrSHNs sequence data were verified and completed using the unpublished Eucalyptus grandis genomic data available in “Eucalyptus grandis Genome Project 2010” (http://www.phytozome.net/eucalyptus), with the permission of Dr. Zander Myburg and his colleagues following the instructions detailed at http://www.phytozome.net/eucalyptus.php.

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Authors and Affiliations

  1. Laboratório de Genômica e Expressão, Departamento de Genética Evolução e Bioagentes, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, CEP 13083-970, Brazil

    Wesley Leoricy Marques, Marcela Mendes Salazar, Eduardo Leal Oliveira Camargo, Jorge Lepikson-Neto, Ricardo Augusto Tiburcio, Leandro Costa do Nascimento & Gonçalo Amarante Guimarães Pereira

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  1. Wesley Leoricy Marques
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  2. Marcela Mendes Salazar
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  3. Eduardo Leal Oliveira Camargo
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  4. Jorge Lepikson-Neto
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  5. Ricardo Augusto Tiburcio
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  6. Leandro Costa do Nascimento
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  7. Gonçalo Amarante Guimarães Pereira
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Correspondence to Gonçalo Amarante Guimarães Pereira.

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Marques, W.L., Salazar, M.M., Camargo, E.L.O. et al. Identification of four Eucalyptus genes potentially involved in cell wall biosynthesis and evolutionarily related to SHINE transcription factors. Plant Growth Regul 69, 203–208 (2013). https://doi.org/10.1007/s10725-012-9754-7

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