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Brassinosteroid analogues effects on the yield and quality parameters of greenhouse-grown pepper (Capsicum annuum L.)

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Abstract

The effects of spraying two brassinosteroid analogues, DI-31 and DI-100, were evaluated at concentrations of 4, 8 and 12 ppm together with a seaweed extract and amino acid mixture called Tomex Amin (2.5 l/ha) to enhance their activity. These were sprayed ten times to foliage of pepper var. Orlando grown under greenhouse conditions. Treatment with DI-31 at a dose of 12 ppm (30 mg/ha) resulted in the highest production increases, which were 13.55 % relative to the control with Tomex Amin (T02). Correlation between net photosynthesis increase and yield increase due to DI-31 treatment, which produced the maximum yield increase and also the highest net photosynthesis with significant differences with respect to the control, was observed. This increased yield was caused by an increase in pepper/plant number. The physic-chemical variables related to pepper quality, such as fresh weight, h/d ratio, lobe number/fruit, firmness, colour and ripening index, were similar in the control and treated peppers. Total antioxidant activity and phenolic content was higher in pepper treated over T02. The results showed that sprayed DI-31 may play an important role in increasing the yield of field grown pepper due to an increase in fruits number per plant without any undesirable effects on their nutritive and organoleptic properties.

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Abbreviations

BB16:

Biobrás 16

BR:

Brassinosteroid

BRs:

Brassinosteroids

RI:

Ripening index

TAA:

Total antioxidant activity

TSS:

Total soluble solids

References

  • Akbudak N, Akbudak B, Seniz V (2007) Foliar applied harpin effects on fruit quality of peppers. Acta Agric Scand Sec B Soil Plant Sci 57:82–86. doi:10.1080/09064710600571622

    CAS  Google Scholar 

  • Ali B, Hayat S, Aiman Hasan S, Ahmad A (2006) Effect of root applied 28-homobrassinolide on the performance of Lycopersicon esculentum. Sci Hortic 110:267–273. doi:10.1016/j.scienta.2006.07.015

    Article  CAS  Google Scholar 

  • Ali B, Hayat S, Ahmad A (2007) 28-Homobrassinolide ameliorates the saline stress in chickpea (Cicer arietinum L.). Environ Exp Bot 59:217–223. doi:10.1016/j.envexpbot.2005.12.002

    Article  CAS  Google Scholar 

  • Amorós A, Pretel MT, Almansa MS, Botella MA, Zapata PJ, Serrano M (2009) Antioxidant and nutritional properties of date fruit from Elche grove as affected by maturation and phenotypic variability of date palm. Food Sci Technol Int 15:65–72. doi:10.1177/1082013208102758

    Article  Google Scholar 

  • Cano A, Hernández-Ruíz J, García-Canovas F, Acosta M, Arnao MB (1998) And end-point method for estimation of the total antioxidant activity in plant material. Phytochem Anal 9:196–202

    Article  CAS  Google Scholar 

  • Churikova VV, Chozhainova GN, Eprintsev AT (1999) The action of epin on the activity of malatdehydrogenase in seedlings of cucumber plants. In: Shevelucha VS, Karlov GI, Karsunkina NP, Salnikova EI, Skorobogatova IV, Siusheva AG (eds) Regulators of plant growth and development 5. Agricultural Academy, Moscow, p 141

    Google Scholar 

  • Coll MF, Jomarrón RIM, Robaina RCM, Alonso BEM, Cabrera PMT (1995) Polyhydroxyspirostanones as plant growth regulators. PCT Int. Appl. CO 7 J 71.100. AOIN 45.00 WO 97.13780

  • Del Amor FM (2006) Growth, photosynthesis and chlorophyll fluorescence of sweet pepper plants as affected by the cultivation method. Ann App Biol 148:133–139. doi:10.1111/j.1744-7348.2006.00048.x

    Article  Google Scholar 

  • Esposito D, Komarnytsky S, Shapses S, Raskin I (2011) Anabolic effect of plant brassinosteroid. FASEB J 25:3708–3719. doi:10.1096/fj.11-181271

    Article  PubMed  CAS  Google Scholar 

  • Fariduddin Q, Hasan SA, Ali B, Hayat S, Ahmad A (2008) Effect of modes of application of 28-homobrassinolide on mung bean. Turkish J Biol 32:17–21

    CAS  Google Scholar 

  • Flores P, Navarro JM, Garrido C, Rubio JS, Martínez V (2004) Influence of Ca2+, K+ and NO3 − fertilisation on nutritional quality of pepper. J Sci Food Agric 84:569–574. doi:10.1002/jsfa.1694

    Article  CAS  Google Scholar 

  • Flores P, Castellar I, Hellín P, Fenoll J, Navarro J (2007) Response of pepper plants to different rates of mineral fertilizers after soil biofumigation and solarisation. J Plant Nutr 30:367–379. doi:10.1080/01904160601171264

    Article  CAS  Google Scholar 

  • Flores P, Hellín P, La Casa A, López A, Fenoll J (2009) Pepper antioxidante compositions affected by organic, low-input and soilles cultivation. J Sci Food Agric 89:2267–2274. doi:10.1002/jsfa.3719

    Article  CAS  Google Scholar 

  • Garcia-Closas R, Berenguer A, Tormo MJ et al (2004) Dietary sources of vitamin C, vitamin E and specific carotenoids in Spain. Brit J Nutr 91:1005–1011. doi:10.1079/BJN20041130

    Article  PubMed  CAS  Google Scholar 

  • Gomes MMA, Compostrini E et al (2006) Brassinosteroid analogue effects on the yield of yellow passion fruit plants (Passiflora edulis f. flavicarpa). Sci Hortic 110:235–240. doi:10.1016/j.scienta.2006.06.030

    Article  CAS  Google Scholar 

  • Hayat S, Ahmad A (2003) Soaking seeds of Lens culinaris with 28-homobrassinolide increased nitrate reductase activity and grain yield in the field in India. Ann Appl Biol 143:121–124. doi:10.1111/j.1744-7348.2003.tb00276.x

    Article  CAS  Google Scholar 

  • Hayat A, Ahmad A, Mobin M, Fariduddin Q, Azam ZM (2001) Carbonic anhydrase, photosynthesis, and seed yield in mustard plants treated with phytohormones. Plant Physiol Sec 39:111–114

    CAS  Google Scholar 

  • Hayat S, Ali B, Hasan A, Ahmad A (2007) Brassinosteroid enhanced the level of antioxidants under cadmium stress in Brassica juncea. Environ Exp Bot 60:33–41. doi:10.1016/j.envexpbot.2006.06.002

    Article  CAS  Google Scholar 

  • Holá D, Rothová O, Koĉová M, Kohout L, Kvasnic M (2010) The effect of brassinosteroids on the morphology, development and yield of field-grown maize. Plant Growth Regul 61:29–43. doi:10.1007/s10725-010-9446-0

    Article  Google Scholar 

  • Hutton MG, Handley DT (2007) Bell pepper cultivar performance under short, variable growing seasons. Hort Technol 17:136–141

    Google Scholar 

  • Janeczko A, Biesaga-Kościelniak J, Oklešt′ková J et al (2010) Role of 24-epibrassinolide in wheat production: physiological effects and uptake. J Agron Crop Sci 196:311–321. doi:10.1111/j.1439-037X.2009.00413.x

    CAS  Google Scholar 

  • Kandelinskaya OL, Topunov AF, Grishchenko ER (2007) Biochemical aspects of growth-stimulating effects of steroid phytohormones on lupine plants. Appl Biochem Micro 43:324–331. doi:10.1134/S0003683807030155

    Article  CAS  Google Scholar 

  • Kang YY, Guo SR (2011) Role of brassinosteroids on horticultural crops. In: Hayat S, Ahmad A (eds) Brassinosteroids,a class of plant hormone. Springer, Berlin, pp 269–288

    Chapter  Google Scholar 

  • Kartal G, Temel A, Arican E, Gozukirmizi N (2009) Effects of brassinosteroids on barley root growth, antioxidant system and cell division. Plant Growth Regul 58:261–267. doi:10.1007/s10725-009-9374-z

    Article  CAS  Google Scholar 

  • Lisso J, Altmann T, Müssing C (2006) Metabolic changes in fruits of the tomato dx mutant. Phytochemistry 67:2232–2238. doi:10.1016/j.phytochem.2006.07.008

    Article  PubMed  CAS  Google Scholar 

  • Lycoskoufis IH, Savvas D, Mavrogianopoulos G (2005) Growth, gas exchange, and nutrient status in pepper (Capsicum annum L.) grown in recirculating nutrient solution as affected by salinity imposed to half of the root system. Sci Hortic 106:147–161. doi:10.1016/j.scienta.2005.02.022

    Article  CAS  Google Scholar 

  • Madeira AC, De Varennes A (2005) Use of chlorophyll meter to assess the effect of nitrogen on sweet pepper development and growth. J Plant Nutr 28:1133–1144. doi:10.1081/PLN-200063133

    Article  CAS  Google Scholar 

  • Marcelis LFM, Heuvelink E, Hofman-Eijer LRB, Bakker JD, Xue LB (2004) Flower and fruit abortion in sweet pepper in relation to source and sink strength. J Exp Bot 55:2261–2268. doi:10.1093/jxb/erh245

    Article  PubMed  CAS  Google Scholar 

  • Mazorra LM, Núñez M, Nápoles MC, Yoshida S, Robaina C, Coll F, Asami T (2004) Effects of structural analogs of brassinosteroids on the recovery of growth inhibition by a specific brassinosteroid biosynthesis inhibitor. Plant Growth Regul 44:183–185

    CAS  Google Scholar 

  • Montoya T, Nomura T, Yokota T et al (2005) Patterns of dwarf expression and brassinosteroid accumulation in tomato reveal the importance of brassinosteroid synthesis during fruit development. Plant J 42:262–269. doi:10.1111/j.1365-313X.2005.02376.x

    Article  PubMed  CAS  Google Scholar 

  • Navarro JM, Flores P, Garrido C, Martinez V (2006) Changes in the contents of antioxidants compounds in pepper fruits at different ripening stages, as affected by salinity. Food Chem 96:66–73. doi:10.1016/j.foodchem.2005.01.057

    Article  CAS  Google Scholar 

  • Núñez M, Torres W, Coll F (1995) Efectividad de un análogo de brasinoesteroide sobre el rendimiento de plantas de papa y tomate. Cultivos Tropicales 16:26–27

    Google Scholar 

  • Núñez M, Robaina C, Coll F (2003) Synthesis and practical applications of brassinosteroid analogs. In: Hayat S, Ahmad A (eds) Brassinosteroids: bioactivity and crop productivity. Kluwer, Dordrecht

    Google Scholar 

  • Papadopoulou E, Grumet R (2005) Brassinosteroid-induced femaleness in cucumber and relationship to ethylene production. Hortic Sci 40:1763–1767

    CAS  Google Scholar 

  • Peng J, Tang X, Feng H (2004) Effects of brassinolide on the physiological properties of litchi pericarp (Litchi chinensis cv. Nuomoci). Sci Hortic 101:407–416. doi:10.1016/j.sicienta.2003.11.012

    Article  CAS  Google Scholar 

  • Pirogovskaya GV, Bogdevitch IM, Naumova GV, Khripach VA, Azizbekyan SG, Krul LP (1996) New forms of mineral fertilizers with additives of plant growth regulators. Proc Plant Growth Regul Soc Am 23:146–151

    Google Scholar 

  • Singh I, Shono M (2005) Physiological and molecular effects of 24-epibrassinolide, a brassinosteroid on thermotolerance of tomato. Plant Growth Regul 47:111–119. doi:10.1007/s10725-005-3252-0

    Article  CAS  Google Scholar 

  • Swamy KN, Rao SSR (2008) Influence of 28-homobrassinolide on growth, photosynthesis metabolite and essential oil content of geranium [Pelargonium graveolens (L.) Herit]. Am J Plant Physiol 3:173–174

    Article  CAS  Google Scholar 

  • Symons GM, Davies C, Shavrukov Y, Dry IB, Reid JB, Thomas, MR (2006) Grapes on steroids. Brassinosteroids are involved in grape berry ripening. Plant Physiol 140: 150–158. doi/10.1104/pp.105.070706

    Google Scholar 

  • Turner AD, Wien HC (1994) Photosynthesis, dark respiration and bud sugar concentrations in pepper cultivars differing in susceptibility to stress-induced bud abscission. Ann Bot 73:623–628

    Article  CAS  Google Scholar 

  • Vardhini BV, Rao SSR (1998) Effect of brassinosteroids on growth, metabolite content and yield of Arachis hypogaea. Phytochemistry 48:927–930

    Article  CAS  Google Scholar 

  • Vardhini BV, Rao SSR (2002) Acceleration of ripening of tomato pericarp discs by brassinosteroids. Phytochemistry 61:843–847

    Article  Google Scholar 

  • Vardhini BV, Anuradha S, Rao SSR (2006) Brassinosteroids-new class of plant hormones with potential to improve crop productivity. Indian J Plant Physiol 11:1–12

    CAS  Google Scholar 

  • Warusavitharana AJ, Tambe TB, Kshirsagar DB (2008) Effect of cytokinins and brassinosteroid with gibberellic acid on yield and quality of Thompson seedless grapes. Acta Hortic 785:217–224

    CAS  Google Scholar 

  • Wood JE, Senthilmohan ST, Peskin AV (2002) Antioxidant activity of procyanidin-containing plant extracts at different pHs. Food Chem 77:155–161

    Article  CAS  Google Scholar 

  • Wu CY, Trieu A, Radhakrishnan P, Kwok SF, Harris S, Zhang K, Wang J, Wan J, Zhai H, Takatsuto S, Matsumoto S, Fujioka S, Feldmann KA, Pennell RI (2008) Brassinosteroids regulate grain filling in rice. Plant Cell 20:2130–2145. doi:10.1105/tpc.107.055087

    Article  PubMed  CAS  Google Scholar 

  • Wubs AM, Heuvelink E, Marcelis LFM (2009) Abortion of reproductive organs in sweet pepper (Capsicum annum L.): a review. J Hortic Sci Biotech 84:467–475

    Google Scholar 

  • Xia XJ, Huang LF, Zhou YH, Mao WH, Shi K, Wu JX, Asami T, Chen Z, Yu JQ (2009) Brassinosteroids promote photosynthesis and growth by enhancing activation of rubisco and expression of photosynthetic genes in Cucumis sativus. Planta 230:1185–1196. doi:10.1007/s00425-009-1016-1

    Article  PubMed  CAS  Google Scholar 

  • Yu JQ, Huang LF, Hu WH, Zhou YH, Mao WH, Ye SF, Nogues S (2004) A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus. J Exp Bot 55:1135–1143. doi:10.1093/jxb/erh124

    Article  PubMed  CAS  Google Scholar 

  • Zhu JH, Li XL, Christie P, Li JL (2005) Environmental implications of low nitrogen use efficiency in excessively fertilized hot pepper (Capsicum frutescens L.) cropping systems. Agr Ecosyst Environ 111:70–80. doi:10.1016/j.agee.2005.04.025

    Article  Google Scholar 

  • Zullo MAT, Adam G (2002) Brassinosteroid phytohormones–structure, bioactivity and applications. Braz J Plant Physiol 14:143–181

    Article  CAS  Google Scholar 

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Acknowledgments

The authors are grateful to the Consellería de Educación of the Generalitat Valenciana for financial support (GV07/016).

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Authors and Affiliations

  1. Department of Applied Biology, Escuela Politécnica Superior de Orihuela, Miguel Hernández University, Ctra. Beniel Km 3.2, 03312, Orihuela, Spain

    Mario Serna & Asunción Amorós

  2. Department of Vegetal Production, Escuela Politécnica Superior de Orihuela, Miguel Hernández University, Ctra. Beniel Km 3.2, 03312, Orihuela, Spain

    Francisca Hernández

  3. Centro de Estudios de Productos Naturales, Facultad de Química, La Habana University, Zapata y G, CP 10400, Vedado, Cuba

    Francisco Coll & Yamilet Coll

Authors
  1. Mario Serna
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  2. Francisca Hernández
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  3. Francisco Coll
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  4. Yamilet Coll
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  5. Asunción Amorós
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Correspondence to Asunción Amorós.

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Serna, M., Hernández, F., Coll, F. et al. Brassinosteroid analogues effects on the yield and quality parameters of greenhouse-grown pepper (Capsicum annuum L.). Plant Growth Regul 68, 333–342 (2012). https://doi.org/10.1007/s10725-012-9718-y

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