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Cloning and functional analysis of CDS_CCI2: a Tanacetum cinerariaefolium chrysanthemyl diphosphate synthase gene

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Abstract

Although irregular monoterpenes are important and common in the Asteraceae family, little is known about their biosynthesis at the genetic level via the MEP pathway. Chrysanthemyl diphosphate (CPP) is an intermediate in the biosynthesis of pyrethrins which are irregular monoterpenes with excellent insecticidal activity in Tanacetum cinerariaefolium (T. cinerariaefolium). In this study, a chrysanthemyl diphosphate synthase (CDS) gene named CDS_CCI2 (GenBank accession no. HQ235057) was isolated from T. cinerariaefolium. It was homologous to T. cinerariaefolium CPP gene family, and proved to be located in the plastid by the in situ subcellular localization. CDS_CCI2 was found to be expressed in roots, stems, leaves, buds and flowers. Moreover, the expression of CDS_CCI2 can be up-regulated by abscisic acid (ABA), methyl jasmonate and ethrel treatment. Phenotypic and molecular analysis showed that overexpression of CDS_CCI2 in micro-tom tomato resulted in dwarf phenotypes characterized with infertile flowers and seedless fruits. Furthermore, overexpression of CDS_CCI2 altered the production of endogenous secondary metabolites. Our data indicate that CPP affects the synthesis of gibberellic acid (GA) and ABA.

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Abbreviations

ABA:

Abscisic acid

CDS:

Chrysanthemyl diphosphate synthase

CPP:

Chrysanthemyl diphosphate

DMAPP:

Dimethylally pyrophosphate

DXR:

1-deoxy-D-xylulose-5-phosphate reductoisomerase

FDS:

Farnesyl diphosphate synthase

GA:

Gibberellic acid

GGDS:

Geranylgeranyl pyrophosphate

G3P:

Glyceraldehyde-3-phosphate

IPP:

Isopentenyl pyrophosphate

MeJA:

Methyl jasmonate

MEP:

2-C-methyl-derythritol-4-phosphate

MS:

Murashige and Skoog

MVA:

Mevalonate

T. cinerariaefoliuma :

Tanacetum cinerariaefolium

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Acknowledgments

This work was supported by the Projects of National Natural Science Foundation of China (30972002, 31071798) and the Committee of Science and Technology of Chongqing (2011BA1024).

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Authors and Affiliations

  1. Genetic Engineering Research Center, Bio-Engineering College, Chongqing University, Chongqing, 400030, China

    Ling Tang, Ji Li, Rasha Khalil, Yingwu Yang, Jing Fan, Mingchun Liu & Zhengguo Li

  2. Chongqing Engineering Research Center for Fungal Insecticides, Key Lab of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission, Chongqing, 400030, China

    Ling Tang, Ji Li, Rasha Khalil, Yingwu Yang, Jing Fan, Mingchun Liu & Zhengguo Li

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  1. Ling Tang
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  2. Ji Li
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Correspondence to Zhengguo Li.

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Tang, L., Li, J., Khalil, R. et al. Cloning and functional analysis of CDS_CCI2: a Tanacetum cinerariaefolium chrysanthemyl diphosphate synthase gene. Plant Growth Regul 67, 161–169 (2012). https://doi.org/10.1007/s10725-012-9673-7

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