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Effects of combinational treatment with ethephon and indole-3-butyric acid on adventitious rooting of Pinus thunbergii cuttings

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Abstract

Adventitious rooting is essential for cutting propagation of pine wilt-resistant Pinus thunbergii. To examine a variety of adventitious rooting potentials among donor plants, cuttings were taken from 31 seedlings within a half-sib family. Rooting abilities of cuttings from each seedling ranged from 0 to 100%. When 11 ortets and 11 ramets (clonally propagated from each ortet) were used as donor plants, there was a positive correlation between rooting abilities of cuttings from ortets and ramets, suggesting that adventitious rooting is dependent on genetic factors in the donor plants. To promote adventitious rooting of cuttings by hormonal treatment, we examined the effect of soaking time in Oxyberon (19.7 mM indole-3-butyric acid (IBA) solution) on rooting. Ten minutes was the best soaking time for rooted cuttings to produce more adventitious roots without impairing normal growth. When cuttings were soaked in Ethrel diluent (69.2 μM ethephon) for 24 h before soaking in Oxyberon for 10 min, a significantly higher rooting ability was observed than those soaked in Oxyberon alone. Ethrel on its own barely affected rooting ability. The positive effect of the combinational treatment was confirmed in a similar experiment using authentic ethephon and IBA instead of Ethrel and Oxyberon. When cuttings were soaked in a mixture of ethephon and silver thiosulfate (STS), an ethylene action inhibitor, before IBA-soaking, the effect was partially diminished compared with combinational treatment without STS. These findings suggest that ethylene action caused by ethephon treatment promotes IBA-mediated adventitious rooting of P. thunbergii cuttings.

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Acknowledgments

The authors would like to thank Prof. S. Shiraishi for useful advice and suggestions on this study. This study was partly funded by the Agriculture, Forestry and Fisheries Research Council of Japan [Grant No. 1680].

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Authors and Affiliations

  1. Fukuoka Prefecture Forest Research and Extension Center, 1438-2, Toyoda Yamamoto-machi, Kurume, Fukuoka, 839-0827, Japan

    Yasuhiro Mori & Fumihiko Miyahara

  2. Department of Forest and Forest Products Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Yasuhiro Mori, Yuji Tsutsumi & Ryuichiro Kondo

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  1. Yasuhiro Mori
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  2. Fumihiko Miyahara
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  3. Yuji Tsutsumi
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  4. Ryuichiro Kondo
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Correspondence to Yuji Tsutsumi.

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Mori, Y., Miyahara, F., Tsutsumi, Y. et al. Effects of combinational treatment with ethephon and indole-3-butyric acid on adventitious rooting of Pinus thunbergii cuttings. Plant Growth Regul 63, 271–278 (2011). https://doi.org/10.1007/s10725-010-9524-3

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